Bacterially expressed human serotonin receptor 3A is functionally reconstituted in proteoliposomes

Protein Expression and Purification

Volumn 88, Issue 2, 2013, Pages 190-195

  Na, Jung Hyun ^a,b   Shin, Jaeil ^c   Jung, Yuna ^d   Lim, Dongbin ^d   Shin, Yeon Kyun ^b,c   Yu, Yeon Gyu ^a  

^a Kookmin University   (South Korea)

^b Korea Institute of Science and Technology   (South Korea)

^c IOWA STATE UNIVERSITY   (United States)

^d Soongsil University   (South Korea)

Author keywords

5 HT3A; Overexpression; Purification; Receptor; Reconstitution; Serotonin

Indexed keywords

CALCIUM; HYBRID PROTEIN; MATRIX PROTEIN; PROTEIN P9, BACTERIOPHAGE PHI6; PROTEOLIPID; PROTEOLIPOSOMES; SEROTONIN; SEROTONIN 3 RECEPTOR;

AFFINITY CHROMATOGRAPHY; ARTICLE; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; UPREGULATION;

CALCIUM; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; PROTEOLIPIDS; RECEPTORS, SEROTONIN, 5-HT3; RECOMBINANT FUSION PROTEINS; SEROTONIN; UP-REGULATION; VIRAL MATRIX PROTEINS;

EID: 84873643442     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2013.01.001     Document Type: Article

References (32)

1. P.B. Bradley, G. Engel, W. Feniuk, J.R. Fozard, P.P. Humphrey, D.N. Middlemiss, E.J. Mylecharane, B.P. Richardson, and P.R. Saxena Proposals for the classification and nomenclature of functional receptors for 5-hydroxytryptamine Neuropharmacology 25 1986 563 676
2. 3 receptor, a serotonin-gated ion channel Science 254 1991 432 437 (Pubitemid 21917361)
3. 3 receptor gene and assignment to human chromosome 11 FEBS Lett. 339 1994 302 306
4. A. Miyake, S. Mochizuki, Y. Takemoto, and S. Akuzawa Molecular cloning of human 5-hydroxytryptamine3 receptor: heterogeneity in distribution and function among species Mol. Pharmacol. 48 1995 407 416
5. F.G. Boess, R. Beroukhim, and I.L. Martin Ultrastructure of the 5-hydroxytryptamine 3 receptor J. Neurochem. 64 1995 1401 1405
6. A. Miyazawa, Y. Fujiyoshi, and N. Unwin Structure and gating mechanism of the acetylcholine receptor pore Nature 423 2003 949 955 (Pubitemid 36806903)
7. N. Unwin Refined structure of the nicotinic acetylcholine receptor at 4 Å resolution J. Mol. Biol. 346 2005 967 989 (Pubitemid 40215523)
8. C.D. Dellisanti, Y. Yao, J.C. Stroud, Z.Z. Wang, and L. Chen Crystal structure of the extracellular domain of nAChR α1 bound to α-bungarotoxin at 1.94 Å resolution Nat. Neurosci. 10 2007 953 962 (Pubitemid 47185209)
9. 3 receptor agonist-binding residues using homology modeling Biophys. J. 64 2003 2338 2344 (Pubitemid 36373556)
10. S.C.R. Lummis, D.L. Beene, L.W. Lee, H.A. Lester, R.W. Broadhurst, and D.A. Dougherty Cis-trans isomerization at a proline opens the pore of a neurotransmitter-gated ion channel Nature 438 2005 248 252 (Pubitemid 41599879)
11. I.M. Paulsen, I.L. Martin, and S.M. Dunn Isomerization of the proline in the M2-M3 linker is not required for activation of the human 5-HT3A receptor J. Neurochem. 110 2009 870 878
12. 3 receptors in rat brain using radioligand binding Nature 330 1987 746 748 (Pubitemid 18017268)
13. S.C.R. Lummis, and I.L. Martini Solubilization, purification, and functional reconstitution of 5-hydroxytryptamine 3 receptors from N1E1-115 neuroblastoma cells Mol. pharmacol. 41 1991 18 23
14. T.P. Roosild, J. Greenwald, M. Vega, S. Castronovo, R. Riek, and S. Choe NMR structure of Mistic, a membrane-integrating protein for membrane protein expression Science 307 2005 1317 1321 (Pubitemid 40299982)
15. D.E. Drew, G. von Heijne, P. Norlund, and J.W.L. de Gier Green fluorescent protein as an indicator to monitor membrane protein overexpression in Escherichia coli FEBS Lett. 507 2001 220 224 (Pubitemid 33001546)
16. A.A. Yeliseev, K.K. Wong, O. Soubias, and K. Gawrisch Expression of human peripheral cannabinoid receptor for structural studies Protein Sci. 14 2005 2638 2653 (Pubitemid 41395590)
17. K. Lee, Y. Jung, J.Y. Lee, W.-K. Lee, D. Lim, and Y.G. Yu Purification and characterization of recombinant human endothelin receptor type A Protein Exp. Purif. 84 2012 14 18
18. Y. Chen, Y. Xu, F. Zhang, and Y.K. Shin Constitutive versus regulated SNARE assembly: a structural basis EMBO J. 23 2004 681 689 (Pubitemid 38418736)
19. X. Zhou, and G. Arthur Improved procedures for the determination of lipid phosphorus by malachite green J. Lipid Res. 33 1992 1233 1236
20. M.D. Johnson III, and L. Mindich Plasmid-directed assembly of the lipid-containing membrane of bacteriophage phi6 J. Bacteriol. 176 1994 4124 4132 (Pubitemid 24191298)
21. 3A) and nicotinic acetylcholine receptors: the anomalous binding properties of nicotine Biochemistry 41 2002 10262 10269 (Pubitemid 34856451)
22. 3AB receptors J. Physiol. 589 2011 4243 4257
23. N. Hussy, W. Lukas, and K.A. Jones Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors J. Physiol. 481 1994 311 323
24. J. Yang Ion permeation through 5-hydroxytryptamine-gated channels in neuroblastoma N18 cells J. Gen. Physiol. 96 1990 1177 1198
25. A. Minta, J.P.Y. Kao, and R.Y. Tsien Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophore J. Biol. Chem. 264 1989 8171 8178 (Pubitemid 19137304)
26. N. Hussy, W. Lukas, and K.A. Jones Functional properties of a cloned 5-hydroxytryptamine ionotropic receptor subunit: comparison with native mouse receptors J. Physiol. 3072 1994 311 323
27. 3 receptor: the role of glutamate residues 97, 224 and 235 J. Biol. Chem. 278 2003 22709 22716 (Pubitemid 36830330)
28. I. Van Wijngaarden, D. Hamminga, R. van Hes, P.J. Standaar, J. Tipker, M.T.M. Tulp, F. Mol, B. Olivier, and A. de Jonge Development of high-affinity 5-HT3 receptor antagonists. Structure-affinity relationships of novel 1,7-annelated indole derivatives J. Med. Chem. 36 1993 3693 3699 (Pubitemid 24004368)
29. 29. K.T. Min, C.L. Wu, and J. Yang Nondepolarizing neuromuscular blockers inhibit the serotonin-type 3A receptor expressed in Xenopus oocytes Anesth. Analg. 90 2000 476 481 (Pubitemid 30078037)
30. 3 receptor from NCB20 cells J. Biol. Chem. 265 1990 13572 13577
31. T. Green, K.A. Stauffer, and S.C.R. Lummis Expression of recombinant homo-oligomeric 5-hydroxytryptamine 3 receptors provides new insights into their maturation and structure J. Biol. Chem. 270 1995 6056 6061
32. J.-L. Eisele, S. Bertrand, J.-L. Galzi, A. Devillers-Thiery, J.-P. Changeux, and D. Bertrand Chimeric nicotinic-serotonergic receptor combines distinct ligand binding and channel specificities Nature 366 1993 479 483 (Pubitemid 24013884)