Optimization of protein buffer cocktails using Thermofluor

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 69, Issue 2, 2013, Pages 209-214

  Reinhard, Linda ^a,b   Mayerhofer, Hubert ^a,c   Geerlof, Arie ^a,d   Mueller Dieckmann, Jochen ^a,e   Weiss, Manfred S ^a,f  

^a DESY   (Germany)

^b AARHUS UNIVERSITY   (Denmark)

^c INSTITUT DE BIOLOGIE STRUCTURALE   (France)

^d INSTITUTE OF EPIDEMIOLOGY   (Germany)

^e UNIVERSITY OF HAMBURG   (Germany)

^f HAHN MEITNER INSTITUT   (Germany)

Author keywords

differential scanning fluorimetry; protein buffer cocktails; protein unfolding; small molecule additives; thermal shift assay; Thermofluor

Indexed keywords

BACTERIAL PROTEIN; BUFFER; PROTEIN;

ARTICLE; BIOASSAY; CHEMISTRY; DIFFERENTIAL SCANNING FLUORIMETRY; ENZYMOLOGY; FLUORESCENCE; METABOLISM; METHODOLOGY; MYCOBACTERIUM TUBERCULOSIS; PROTEIN BUFFER COCKTAILS; PROTEIN UNFOLDING; SMALL-MOLECULE ADDITIVES; SOLUTION AND SOLUBILITY; TEMPERATURE; THERMAL SHIFT ASSAY; THERMOFLUOR;

DIFFERENTIAL SCANNING FLUORIMETRY; PROTEIN BUFFER COCKTAILS; PROTEIN UNFOLDING; SMALL-MOLECULE ADDITIVES; THERMAL SHIFT ASSAY; THERMOFLUOR;

BACTERIAL PROTEINS; BIOLOGICAL ASSAY; BUFFERS; FLUORESCENCE; MYCOBACTERIUM TUBERCULOSIS; PROTEIN UNFOLDING; PROTEINS; SOLUTIONS; TEMPERATURE;

EID: 84873590925     PISSN: None     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309112051858     Document Type: Article

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