Platelets express and release osteocalcin and co-localize in human calcified atherosclerotic plaques

Journal of Thrombosis and Haemostasis

Volumn 11, Issue 2, 2013, Pages 357-365

  Foresta, C ^a   Strapazzon, G ^a,b   De Toni, L ^a   Fabris, F ^c   Grego, F ^a   Gerosa, G ^a   Vettore, S ^c   Garolla, A ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b INSTITUTE FOR RENEWABLE ENERGY   (Italy)

^c UNIVERSITY OF PADUA MEDICAL SCHOOL   (Italy)

Author keywords

Atherosclerosis; Megakaryocyte; Osteocalcin; Platelets; Vascular calcification

Indexed keywords

CD63 ANTIGEN; OSTEOCALCIN;

ADULT; ARTICLE; ATHEROSCLEROTIC PLAQUE; CAROTID ARTERY OBSTRUCTION; CAROTID ENDARTERECTOMY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; IMMUNOFLUORESCENCE; MALE; MEGAKARYOCYTE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; THROMBOCYTE; THROMBOCYTE ACTIVATION; THROMBOCYTE AGGREGATION; WESTERN BLOTTING;

ANTIGENS, CD63; BLOOD PLATELETS; BLOTTING, WESTERN; CAROTID ARTERY DISEASES; CASE-CONTROL STUDIES; CELL SEPARATION; ENDARTERECTOMY, CAROTID; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; HUMANS; MALE; MEGAKARYOCYTES; OSTEOCALCIN; PLAQUE, ATHEROSCLEROTIC; PLATELET ACTIVATION; PLATELET MEMBRANE GLYCOPROTEIN IIB; SECRETORY VESICLES; VASCULAR CALCIFICATION;

EID: 84873532131     PISSN: 15387933     EISSN: 15387836     Source Type: Journal    
DOI: 10.1111/jth.12088     Document Type: Article

References (30)

1. Gawaz M, Langer H, May AE. Platelets in inflammation and atherogenesis. J Clin Invest 2005; 115: 3378-84.
2. Libby P. Inflammation in atherosclerosis. Nature 2002; 420: 868-74.
3. Elkeles RS. Coronary artery calcium and cardiovascular risk in diabetes. Atherosclerosis 2010; 210: 331-6.
4. Fadini GP, Rattazzi M, Matsumoto T, Asahara T, Khosla S. Emerging role of circulating calcifying cells in the bone-vascular axis. Circulation 2012; 125: 2772-81.
5. Bini A, Mann KG, Kudryk BJ, Schoen FJ. Noncollagenous bone matrix proteins, calcification, and thrombosis in carotid artery atherosclerosis. Arterioscler Thromb Vasc Biol 1999; 19: 1852-61.
6. Dhore CR, Cleutjens JP, Lutgens E, Cleutjens KB, Geusens PP, Kitslaar PJ, Tordoir JH, Spronk HM, Vermeer C, Daemen MJ. Differential expression of bone matrix regulatory proteins in human atherosclerotic plaques. Arterioscler Thromb Vasc Biol 2001; 21: 1998-2003.
7. Gössl M, Mödder UI, Atkinson EJ, Lerman A, Khosla S. Osteocalcin expression by circulating endothelial progenitor cells in patients with coronary atherosclerosis. J Am Coll Cardiol 2008; 52: 1314-25.
8. Fadini GP, Albiero M, Menegazzo L, Boscaro E, Vigili de Kreutzenberg S, Agostini C, Cabrelle A, Binotto G, Rattazzi M, Bertacco E, Bertorelle R, Biasini L, Mion M, Plebani M, Ceolotto G, Angelini A, Castellani C, Menegolo M, Grego F, Dimmeler S, et al. Widespread increase in myeloid calcifying cells contributes to ectopic vascular calcification in type 2 diabetes. Circ Res 2011; 108: 1112-21.
9. Johnson RC, Leopold JA, Loscalzo J. Vascular calcification: pathobiological mechanisms and clinical implications. Circ Res 2006; 99: 1044-59. Erratum in: Circ Res 2009; 105:e8.
10. Stenner DD, Tracy RP, Riggs BL, Mann KG. Human platelets contain and secrete osteonectin, a major protein of mineralized bone. Proc Natl Acad Sci U S A 1986; 83: 6892-6.
11. Coppinger JA, Cagney G, Toomey S, Kislinger T, Belton O, McRedmond JP, Cahill DJ, Emili A, Fitzgerald DJ, Maguire PB. Characterization of the proteins released from activated platelets leads to localization of novel platelet proteins in human atherosclerotic lesions. Blood 2004; 103: 2096-104.
12. Thiede MA, Smock SL, Petersen DN, Grasser WA, Thompson DD, Nishimoto SK. Presence of messenger ribonucleic acid encoding osteocalcin, a marker of bone turnover, in bone marrow megakaryocytes and peripheral blood platelets. Endocrinology 1994; 135: 929-37.
13. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2010; 33: S62-9.
14. Rubenstein DA, Yin W. Glycated albumin modulates platelet susceptibility to flow induced activation and aggregation. Platelets 2009; 20: 206-15.
15. Pi M, Faber P, Ekema G, Jackson PD, Ting A, Wang N, Fontilla-Poole M, Mays RW, Brunden KR, Harrington JJ, Quarles LD. Identification of a novel extracellular cation-sensing G-protein-coupled receptor. J Biol Chem 2005; 280: 40201-9.
16. McGrath RT, McRae E, Smith OP, O'Donnell JS. Platelet von Willebrand factor--structure, function and biological importance. Br J Haematol 2010; 148: 834-43.
17. Viisoreanu D, Gear A. Effect of physiologic shear stresses and calcium on agonist-induced platelet aggregation, secretion, and thromboxane A2 formation. Thromb Res 2007; 120: 885-92.
18. Ramström AS, Fagerberg IH, Lindahl TL. A flow cytometric assay for the study of dense granule storage and release in human platelets. Platelets 1999; 10: 153-8.
19. Uzui H, Harpf A, Liu M, Doherty TM, Shukla A, Chai NN, Tripathi PV, Jovinge S, Wilkin DJ, Asotra K, Shah PK, Rajavashisth TB. Increased expression of membrane type 3-matrix metalloproteinase in human atherosclerotic plaque: role of activated macrophages and inflammatory cytokines. Circulation 2002; 106: 3024-30.
20. Borissoff JI, Spronk HM, ten Cate H. The hemostatic system as a modulator of atherosclerosis. N Engl J Med 2011; 364: 1746-60.
21. Owen DR, Lindsay AC, Choudhury RP, Fayad ZA. Imaging of atherosclerosis. Annu Rev Med 2011; 62: 25-40.
22. Price PA, Thomas GR, Pardini AW, Figueira WF, Caputo JM, Williamson MK. Discovery of a high molecular weight complex of calcium, phosphate, fetuin, and matrix gamma-carboxyglutamic acid protein in the serum of etidronate-treated rats. J Biol Chem 2002; 277: 3926-34.
23. Neven E, D'Haese PC. Vascular calcification in chronic renal failure: what have we learned from animal studies?Circ Res 2011; 108: 249-64.
24. Idelevich A, Rais Y, Monsonego-Ornan E. Bone Gla protein increases HIF-1{alpha}-dependent glucose metabolism and induces cartilage and vascular calcification. Arterioscler Thromb Vasc Biol 2011; 31: e55-71.
25. Eghbali-Fatourechi GZ, Lamsam J, Fraser D, Nagel D, Riggs BL, Khosla S. Circulating osteoblast-lineage cells in humans. N Engl J Med 2005; 352: 1959-66.
26. Foresta C, De Toni L, Biagioli A, Ganz F, Magagna S, Caretta N. Increased levels of osteocalcin-positive endothelial progenitor cells in patients affected by erectile dysfunction and cavernous atherosclerosis. J Sex Med 2010; 7: 751-7.
27. Virmani R, Burke AP, Farb A, Kolodgie FD. Pathology of the vulnerable plaque. J Am Coll Cardiol 2006; 47: C13-8.
28. Flammer AJ, Gössl M, Widmer RJ, Reriani M, Lennon R, Loeffler D, Shonyo S, Simari RD, Lerman LO, Khosla S, Lerman A. Osteocalcin positive CD133+/CD34-/KDR+ progenitor cells as an independent marker for unstable atherosclerosis. Eur Heart J 2012; 33: 2963-9. Epub ahead of print.
29. Fadini GP. Is bone marrow another target of diabetic complications?Eur J Clin Invest 2011; 41: 457-63.
30. Pallotta I, Lovett M, Rice W, Kaplan DL, Balduini A. Bone marrow osteoblastic niche: a new model to study physiological regulation of megakaryopoiesis. PLoS ONE 2009; 4: e8359.