Comparison of different mass spectrometry techniques in the measurement of L-[ring-13C6]phenylalanine incorporation into mixed muscle proteins

Journal of Mass Spectrometry

Volumn 48, Issue 2, 2013, Pages 269-275

  Zabielski, Piotr ^a   Ford, G Charles ^a   Persson, X Mai ^a   Jaleel, Abdul ^a   Dewey, Jerry D ^a   Nair, K Sreekumaran ^a  

^a MAYO CLINIC   (United States)

Author keywords

amino acid tracer; GC C IRMS; GC MS; GC MS MS ; L ring 13C6 phenylalanine enrichment; LC MS MS; mixed muscle proteins; tandem mass spectrometry

Indexed keywords

AMINO-ACID TRACER; GC/C/IRMS; GC/MS; GC/MS/MS; LC/MS/MS; MUSCLE PROTEINS; TANDEM MASS SPECTROMETRY;

FACTOR ANALYSIS; GAS CHROMATOGRAPHY; ISOTOPES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MUSCLE;

AMINO ACIDS;

MUSCLE PROTEIN; PHENYLALANINE DERIVATIVE; STABLE ISOTOPE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; COMBUSTION ISOTOPE RATIO MASS SPECTROMETRY; CONTROLLED STUDY; GAS CHROMATOGRAPHY; HUMAN; HUMAN EXPERIMENT; HUMAN TISSUE; INTERMETHOD COMPARISON; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MALE; MASS FRAGMENTOGRAPHY; MASS SPECTROMETRY; MOUSE; MUSCLE TISSUE; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; RAT; TANDEM MASS SPECTROMETRY;

ANIMALS; CARBON ISOTOPES; CHROMATOGRAPHY, LIQUID; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; HUMANS; ISOTOPE LABELING; LINEAR MODELS; MALE; MUSCLE PROTEINS; MUSCLE, SKELETAL; PHENYLALANINE; RADIOACTIVE TRACERS; RATS; REPRODUCIBILITY OF RESULTS; TANDEM MASS SPECTROMETRY;

EID: 84873347914     PISSN: 10765174     EISSN: 10969888     Source Type: Journal    
DOI: 10.1002/jms.3120     Document Type: Article

References (24)

1. 15 N)lysine. Clin. Sci. Mol. Med. 1975, 49, 581.
2. K. S. Nair, S. L. Welle, D. Halliday, R. G. Campbell,. Effect of beta-hydroxybutyrate on whole-body leucine kinetics and fractional mixed skeletal muscle protein synthesis in humans. J. Clin. Invest. 1988, 82, 198.
3. M. J. Rennie, R. H. Edwards, P. W. Emery, D. Halliday, K. Lundholm, D. J. Millward,. Depressed protein synthesis is the dominant characteristic of muscle wasting and cachexia. Clin. Physiol. 1983, 3, 387.
4. E. Volpi, A. A. Ferrando, C. W. Yeckel, K. D. Tipton, R. R. Wolfe,. Exogenous amino acids stimulate net muscle protein synthesis in the elderly. J. Clin. Invest. 1998, 101, 2000.
5. K. R. Short, J. L. Vittone, M. L. Bigelow, D. N. Proctor, K. S. Nair,. Age and aerobic exercise training effects on whole body and muscle protein metabolism. Am. J. Physiol. Endocrinol. Metab. 2004, 286, E92.
6. I. G. Brodsky, P. Balagopal, K. S. Nair,. Effects of testosterone replacement on muscle mass and muscle protein synthesis in hypogonadal men - a clinical research center study. J. Clin. Endocrinol. Metab. 1996, 81, 3469.
7. P. Balagopal, J. C. Schimke, P. Ades, D. Adey, K. S. Nair,. Age effect on transcript levels and synthesis rate of muscle MHC and response to resistance exercise. Am. J. Physiol. Endocrinol. Metab. 2001, 280, E203.
8. K. S. Nair, D. Halliday, R. C. Griggs,. Leucine incorporation into mixed skeletal muscle protein in humans. Am. J. Physiol. 1988, 254, E208.
9. P. Q. Baumann, W. S. Stirewalt, B. D. O'Rourke, D. Howard, K. S. Nair,. Precursor pools of protein synthesis: a stable isotope study in a swine model. Am. J. Physiol. 1994, 267, E203.
10. P. Balagopal, K. S. Nair, W. S. Stirewalt,. Isolation of myosin heavy chain from small skeletal muscle samples by preparative continuous elution gel electrophoresis: application to measurement of synthesis rate in human and animal tissue. Anal. Biochem. 1994, 221, 72.
11. B. W. Patterson, X. J. Zhang, Y. Chen, S. Klein, R. R. Wolfe,. Measurement of very low stable isotope enrichments by gas chromatography/mass spectrometry: application to measurement of muscle protein synthesis. Metabolism 1997, 46, 943.
12. L. S. Chow, R. C. Albright, M. L. Bigelow, G. Toffolo, C. Cobelli, K. S. Nair,. Mechanism of insulin's anabolic effect on muscle: measurements of muscle protein synthesis and breakdown using aminoacyl-tRNA and other surrogate measures. Am. J. Physiol. Endocrinol. Metab. 2006, 291, E729.
13. A. G. Calder, S. E. Anderson, I. Grant, M. A. McNurlan, P. J. Garlick,. The determination of low d5-phenylalanine enrichment (0.002-0.09 atom percent excess), after conversion to phenylethylamine, in relation to protein turnover studies by gas chromatography/electron ionization mass spectrometry. Rapid Commun. Mass Spectrom. 1992, 6, 421.
14. 5]phenylalanine at very low enrichment: Measurement of protein synthesis in health and disease. J. Mass Spectrom. 1995, 30, 1325.
15. X. M. Persson, A. U. Blachnio-Zabielska, M. D. Jensen,. Rapid measurement of plasma free fatty acid concentration and isotopic enrichment using LC/MS. J. Lipid Res. 2010, 51, 2761.
16. A. Jaleel, V. Nehra, X. M. Persson, Y. Boirie, M. Bigelow, K. S. Nair,. In vivo measurement of synthesis rate of multiple plasma proteins in humans. Am. J. Physiol. Endocrinol. Metab. 2006, 291, E190.
17. A. Jaleel, K. A. Klaus, D. M. Morse, H. Karakelides, L. E. Ward, B. A. Irving, K. S. Nair,. Differential effects of insulin deprivation and systemic insulin treatment on plasma protein synthesis in type 1 diabetic people. Am. J. Physiol. Endocrinol. Metab. 2009, 297, E889.
18. A. Jaleel, G. C. Henderson, B. J. Madden, K. A. Klaus, D. M. Morse, S. Gopala, K. S. Nair,. Identification of de novo synthesized and relatively older proteins: accelerated oxidative damage to de novo synthesized apolipoprotein A-1 in type 1 diabetes. Diabetes 2010, 59, 2366.
19. O. H. Ljungqvist, M. Persson, G. C. Ford, K. S. Nair,. Functional heterogeneity of leucine pools in human skeletal muscle. Am. J. Physiol. 1997, 273, E564.
20. 15 N isotopic abundances in physiological samples: a pilot study on amino acid synthesis in the upper gastro-intestinal tract of minipigs. J. Mass Spectrom. 1996, 31, 367.
21. G. C. Ford, K. N. Cheng, D. Halliday,. Analysis of (1-13C)leucine and (13C)KIC in plasma by capillary gas chromatography/mass spectrometry in protein turnover studies. Biomed. Mass Spectrom. 1985, 12, 432.
22. D. Halliday, W. W. Read,. Mass spectrometric assay of stable isotopic enrichment for the estimation of protein turnover in man. Proc. Nutr. Soc. 1981, 40, 321.
23. R. J. Meesters, R. R. Wolfe, N. E. Deutz,. Application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the analysis of stable isotope enrichments of phenylalanine and tyrosine. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2009, 877, 43.
24. A. Jaleel, K. R. Short, Y. W. Asmann, K. A. Klaus, D. M. Morse, G. C. Ford, K. S. Nair,. In vivo measurement of synthesis rate of individual skeletal muscle mitochondrial proteins. Am. J. Physiol. Endocrinol. Metab. 2008, 295, E1255.