Affinity grid-based cryo-EM of PKC binding to RACK1 on the ribosome

Journal of Structural Biology

Volumn 181, Issue 2, 2013, Pages 190-194

  Sharma, Gyanesh ^a   Pallesen, Jesper ^a   Das, Sanchaita ^a   Grassucci, Robert ^a   Langlois, Robert ^a   Hampton, Cheri M ^a   Kelly, Deborah F ^b   des Georges, Amedee ^a   Frank, Joachim ^a  

^a Columbia University ^*  (United States)

^b VIRGINIA TECH CARILION SCHOOL OF MEDICINE   (United States)

Author keywords

Affinity grid; Cryo EM; PKC II; RACK1; Ribosome

Indexed keywords

POLYHISTIDINE TAG; PROTEIN KINASE C BETA II; PROTEIN SERINE THREONINE KINASE; RECEPTOR FOR ACTIVATED C KINASE 1; UNCLASSIFIED DRUG;

AFFINITY GRID; ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ELECTRON TOMOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; RABBIT; RETICULOCYTE; SMALL RIBOSOMAL SUBUNIT;

CRYOELECTRON MICROSCOPY; GTP-BINDING PROTEINS; HUMANS; MODELS, MOLECULAR; NEOPLASM PROTEINS; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; PROTEIN KINASE C; RECEPTORS, CELL SURFACE; RIBOSOME SUBUNITS, SMALL, EUKARYOTIC;

EID: 84873178696     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2012.11.006     Document Type: Article

References (24)

1. Adams D.R., Ron D., Kiely P.A. RACK1, a multifaceted scaffolding protein: structure and function. Cell Commun. Signal. 2011, 9:22.
2. Ben-Shem A., Jenner L., Yusupova G., Yusupov M. Crystal structure of the eukaryotic ribosome. Science 2010, 330:1203-1209.
3. Frank J., Radermacher M., Penczek P., Zhu J., Li Y., et al. SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J. Struct. Biol. 1996, 116:190-199.
4. Grosso S., Volta V., Vietri M., Gorrini C., Marchisio P.C., et al. Eukaryotic ribosomes host PKC activity. Biochem. Biophys. Res. Commun. 2008, 376:65-69.
5. Grosso S., Volta V., Sala L.A., Vietri M., Marchisio P.C., et al. PKCbetaII modulates translation independently from mTOR and through RACK1. Biochem. J. 2008, 415:77-85.
6. Kelly D.F., Dukovski D., Walz T. Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy. Proc. Natl. Acad. Sci. USA 2008, 105:4703-4708.
7. Kelly D.F., Abeyrathne P.D., Dukovski D., Walz T. The affinity grid: a pre-fabricated EM grid for monolayer purification. J. Mol. Biol. 2008, 382:423-433.
8. Kelly D.F., Dukovski D., Walz T. Strategy for the use of affinity grids to prepare non-His-tagged macromolecular complexes for single-particle electron microscopy. J. Mol. Biol. 2010, 400:675-681.
9. Kelly D.F., Dukovski D., Walz T. A practical guide to the use of monolayer purification and affinity grids. Methods Enzymol. 2010, 481:83-107.
10. Leonard T.A., Rozycki B., Saidi L.F., Hummer G., Hurley J.H. Crystal structure and allosteric activation of protein kinase C betaII. Cell 2011, 144:55-66.
11. Li D., Roberts R. WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. Cell Mol. Life Sci. 2001, 58:2085-2097.
12. Liu Y.V., Hubbi M.E., Pan F., McDonald K.R., Mansharamani M., et al. Calcineurin promotes hypoxia-inducible factor 1alpha expression by dephosphorylating RACK1 and blocking RACK1 dimerization. J. Biol. Chem. 2007, 282:37064-37073.
13. McCahill A., Warwicker J., Bolger G.B., Houslay M.D., Yarwood S.J. The RACK1 scaffold protein: a dynamic cog in cell response mechanisms. Mol. Pharmacol. 2002, 62:1261-1273.
14. Miluzio A., Beugnet A., Volta V., Biffo S. Eukaryotic initiation factor 6 mediates a continuum between 60S ribosome biogenesis and translation. EMBO Rep. 2009, 10:459-465.
15. Nilsson J., Sengupta J., Frank J., Nissen P. Regulation of eukaryotic translation by the RACK1 protein: a platform for signalling molecules on the ribosome. EMBO Rep. 2004, 5:1137-1141.
16. Ron D., Mochly-Rosen D. An autoregulatory region in protein kinase C: the pseudoanchoring site. Proc. Natl. Acad. Sci. USA 1995, 92:492-496.
17. Ron D., Chen C.H., Caldwell J., Jamieson L., Orr E., et al. Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins. Proc. Natl. Acad. Sci. USA 1994, 91:839-843.
18. Ron D., Luo J., Mochly-Rosen D. C2 region-derived peptides inhibit translocation and function of beta protein kinase C in vivo. J. Biol. Chem. 1995, 270:24180-24187.
19. Ron D., Jiang Z., Yao L., Vagts A., Diamond I., et al. Coordinated movement of RACK1 with activated betaIIPKC. J. Biol. Chem. 1999, 274:27039-27046.
20. Schechtman D., Mochly-Rosen D. Adaptor proteins in protein kinase C-mediated signal transduction. Oncogene 2001, 20:6339-6347.
21. Sengupta J., Nilsson J., Gursky R., Spahn C.M., Nissen P., et al. Identification of the versatile scaffold protein RACK1 on the eukaryotic ribosome by cryo-EM. Nat. Struct. Mol. Biol. 2004, 11:957-962.
22. Sondek J., Siderovski D.P. Ggamma-like (GGL) domains: new frontiers in G-protein signaling and beta-propeller scaffolding. Biochem. Pharmacol. 2001, 61:1329-1337.
23. Stebbins E.G., Mochly-Rosen D. Binding specificity for RACK1 resides in the V5 region of beta II protein kinase C. J. Biol. Chem. 2001, 276:29644-29650.
24. Yatime L., Hein K.L., Nilsson J., Nissen P. Structure of the RACK1 dimer from Saccharomyces cerevisiae. J. Mol. Biol. 2011, 411:486-498.