Functional expression, purification and reconstitution of the recombinant phosphate transporter Pho89 of Saccharomyces cerevisiae

FEBS Journal

Volumn 280, Issue 3, 2013, Pages 965-975

  Sengottaiyan, Palanivelu ^a   Ruiz Pavõn, Lorena ^a   Persson, Bengt L ^a,b,c  

^a LINNAEUS UNIVERSITY   (Sweden)

^b UNIVERSITY OF LEUVEN   (Belgium)

^c DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

Author keywords

Pho89; phosphate transport reconstitution; Pichia pastoris; Saccharomyces cerevisiae

Indexed keywords

DIMER; MONENSIN; MONOMER; OLIGOMER; PHOSPHATE TRANSPORTER; PROTEOLIPOSOME; RECOMBINANT PHO89 PROTEIN; RECOMBINANT PROTEIN; SODIUM ION; TRITON X 100; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; ELUTION; GEL FILTRATION; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PHOSPHATE TRANSPORT; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SOLUBILITY; WESTERN BLOTTING;

BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELL MEMBRANE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; ELECTROCHEMICAL TECHNIQUES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR WEIGHT; MONENSIN; OCTOXYNOL; PHOSPHATES; PHOSPHORYLCHOLINE; PICHIA; PROTEIN MULTIMERIZATION; PROTEOLIPIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SODIUM IONOPHORES; SODIUM-PHOSPHATE COTRANSPORTER PROTEINS, TYPE III; SOLUBILITY;

PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 84873160670     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/febs.12090     Document Type: Article

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