Development and validation of a simple assay for the determination of cholinesterase activity in whole blood of laboratory animals

Journal of Applied Toxicology

Volumn 33, Issue 4, 2013, Pages 290-300

  Naik, Ramachandra S ^a   Liu, Weiyi ^a   Saxena, Ashima ^a  

^a WALTER REED ARMY INSTITUTE OF RESEARCH   (United States)

Author keywords

( ) huperzine A; Acetylcholinesterase; Animal whole blood; Butyrylcholinesterase; Cholinesterase activity; Cholinesterase inhibitors; Ethopropazine

Indexed keywords

CHOLINESTERASE; CHOLINESTERASE INHIBITOR; HEMOGLOBIN; HUPERZINE A; PROFENAMINE;

ACCURACY; ANIMAL EXPERIMENT; ARTICLE; BLOOD; BLOOD SAMPLING; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ERYTHROCYTE; HEMOGLOBIN BLOOD LEVEL; HUMAN; HYDROLYSIS; MOUSE; NONHUMAN; PRIORITY JOURNAL; RAT; REPRODUCIBILITY;

ALKALOIDS; ANIMALS; ANIMALS, LABORATORY; CERCOPITHECUS AETHIOPS; CHOLINESTERASE INHIBITORS; CHOLINESTERASES; DOGS; GUINEA PIGS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; LIMIT OF DETECTION; MACACA FASCICULARIS; MACACA MULATTA; MICE; MICE, INBRED BALB C; PHENOTHIAZINES; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; SESQUITERPENES; SWINE; SWINE, MINIATURE;

ANIMALIA; CANIS FAMILIARIS; CAVIA; MACACA MULATTA; MUS; RATTUS;

EID: 84873097643     PISSN: 0260437X     EISSN: 10991263     Source Type: Journal    
DOI: 10.1002/jat.2730     Document Type: Article

References (44)

1. Aldridge WN. 1953. The differentiation of true and pseudo cholinesterase by organophosphorus compounds. Biochem. J. 53: 62-67.
2. Ashani Y, Shapira S, Levy D, Wolfe AD, Doctor BP, Raveh L. 1991. Butyrylcholinesterase and acetylcholinesterase prophylaxis against soman poisoning in mice. Biochem. Pharmacol. 41: 37-41.
3. Augustinsson KB. 1957. Assay methods for cholinesterases. Meth. Biochem. Anal. 5: 1-63.
4. Augustinsson KB, Eriksson H, Faijersson Y. 1978. A new approach to determining cholinesterase activities in samples of whole blood. Clin. Chim. Acta 89: 239-252.
5. Austin L, Berry WK. 1953. Two selective inhibitors of cholinesterase. Biochem. J. 54: 695-700.
6. Bartels CF, Xie W, Miller-Lindholm AK, Schofer LM, Lockridge O. 2000. Determination of the DNA sequences of acetylcholinesterase and butyrylcholinesterase from cat and demonstration of the existence of both in cat plasma. Biochem. Pharmacol. 60: 479-487; doi: 10.1016/S0006-2952(00)00365-8.
7. Brzin M, Dettbarn WD, Rosenberg P, Nachmansohn D. 1965. Cholinesterase activity per unit surface area of conducting membranes. J. Cell Biol. 26: 353-364.
8. Christin D, Daulon S, Delamanche S, Perrier N, Breton P, Taysse L. 2008. Effects of repeated low-dose soman exposure on monoamine levels in different brain structures in mice. Neurochem. Res. 33: 919-926; doi: 10.1007/s11064-007-9535-2.
9. Coye MJ, Lowe JA, Maddy KT. 1986. Biological monitoring of agricultural workers exposed to pesticides: I. Cholinesterase activity determinations. J. Occup. Med. 28: 619-627.
10. Dabisch PA, Burnett DC, Miller DB, Jakubowski EM, Muse WT, Forster JS, Scotto JA, Jarvis JR, Davis EA, Hulet SW, Reutter SA, Mioduszewski RJ, Thomson SA. 2005. Tolerance to the miotic effect of sarin vapor in rats after multiple low-level exposures. J. Ocul. Pharmacol. Ther. 21: 182-195.
11. Doctor BP, Toker L, Roth E, Silman I. 1987. Microtiter assay for acetylcholinesterase. Anal. Biochem. 166: 399-403.
12. Ellman GL, Courtney KD, Andres V Jr., Feather-Stone RM. 1961. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7: 88-95.
13. Eyer P, Worek F, Kiderlen D, Sinko G, Stuglin A, Simeon-Rudolf V, Reiner E. 2003. Molar absorption coefficients for the reduced Ellman reagent: reassessment. Anal. Biochem. 312: 224-227; doi: 10.1016/S0003-2697(02)00506-7.
14. Genovese RF, Oubre JL, Jakubowski EM, Fleming PJ, Saxena A, Rockwood GA, Tipparaju P, Willmore CB. 2007. Evaluation of cognitive and biochemical effects of low-level exposure to sarin in rhesus and African green monkeys. Toxicology 231: 11-20; doi: 10.1016/j.tox.2006.10.018.
15. Genovese RF, Benton BJ, Johnson CC, Jakubowski EM. 2009. Assessment of low level whole-body soman vapor exposure in rats. Neurotoxicol. Teratol. 31: 110-118; doi: 10.1016/j.ntt.2008.10.003.
16. Genovese RF, Sun W, Johnson CC, Ditargiani RC, Doctor BP, Saxena A. 2010. Safety of administration of human butyrylcholinesterase and its conjugates with soman or VX in rats. Basic Clin. Pharmacol. Toxicol. 106: 428-434.
17. Gordon RK, Haigh JR, Garcia GE, Feaster SR, Riel MA, Lenz Aisen, PS, Doctor BP. 2005. Oral administration of pyridostigmine bromide and huperzine A protects human whole blood cholinesterases from ex vivo exposure to soman. Chem. Biol. Interact. 157-158: 239-246; doi: 10.1016/j.cbi.2005.10.031.
18. Hawkins RD, Mendel B. 1949. Studies on cholinesterase. 6. The selective inhibition of true cholinesterase in vivo. Biochem. J. 44: 260-264.
19. Kolf-Clauw M, Jez S, Ponsart C, Delamanche IS. 2000. Acetyl- and pseudo-cholinesterase activities of plasma, erythrocytes, and whole blood in male beagle dogs using Ellman's assay. Vet. Hum. Toxicol. 42: 216-219.
20. Lassiter TL, Marshall RS, Jackson LC, Hunter DL, Vu JT, Padilla S. 2003. Automated measurement of acetylcholinesterase activity in rat peripheral tissues. Toxicology 186: 241-253; doi: 10.1016/S0300-483X(02)00752-7.
21. Lewis MK, Eldefrawi ME. 1974. A simple, rapid, and quantitative radiometric assay of acetylcholinesterase. Anal. Biochem. 57: 588-592.
22. Li B, Sedlacek M, Manoharan I, Boopathy R, Duysen EG, Masson P, Lockridge O. 2005. Butyrylcholinesterase, paraoxonase, and albumin esterase, but not carboxylesterase, are present in human plasma. Biochem. Pharmacol. 70: 1673-1684; doi: 10.1016/j.bcp.2005.09.002.
23. Lotti M. 1995. Cholinesterase inhibition: complexities in interpretation. Clin. Chem. 41: 1814-1818.
24. Mikalsen A, Andersen RA, Alexander J. 1986. Use of ethopropazine and BW 284C51 as selective inhibitors for cholinesterases from various species. Comp. Biochem. Physiol. 83: 447-449.
25. Munoz Delgado E, Gomez-Fernandez JC, Mayorga OL, Lozano JA, Vidal CJ. 1983. An ultrasensitive electrometric system to measure membrane-bound acetylcholinesterase activity. Anal. Biochem. 133: 302-306.
26. Nabb DP, Whitfield F. 1967. Determination of cholinesterase by an automated pH stat method. Arch. Environ. Health 15: 147-154.
27. Naik RS, Doctor BP, Saxena A. 2008. Comparison of methods used for the determination of cholinesterase activity in whole blood. Chem. Biol. Interact. 175: 298-302; doi:10.1016/j.cbi.2008.05.002.00
28. Oliveira-Silva JJ, Alves SR, Inacio AF, Meyer A, Sarcinelli PN, Mattos RC, Ferreira MF, Cunha JC, Moreira JC. 2000. Cholinesterase activities determination in frozen blood samples: an improvement to the occupational monitoring in developing countries. Hum. Exp. Toxicol. 19: 173-177.
29. Radic Z, Reiner E, Taylor P. 1991. Role of the peripheral anionic site on acetylcholinesterase: inhibition by substrates and coumarin derivatives. Mol. Pharmacol. 39: 98-104.
30. Reiner E, Sinko G, Skrinjaric-Spoljar M, Simeon-Rudolf V. 2000. Comparison of protocols for measuring activities of human blood cholinesterases by the Ellman method. Arh. Hig. Rada Toksikol. 51: 13-18.
31. Reiner E, Bosak A, Simeon-Rudolf V. 2004. Activity of cholinesterases in human whole blood measured with acetylthiocholine as substrate and ethopropazine as selective inhibitor of plasma butyrylcholinesterase. Arh. Hig. Rada Toksikol. 55: 1-4.
32. Sanchez-Hernandez JC. 2003. Evaluating reptile exposure to cholinesterase-inhibiting agrochemicals by serum butyrylcholinesterase activity. Environ. Toxicol. Chem. 22: 296-301.
33. Saxena A, Redman AM, Jiang X, Lockridge O, Doctor BP. 1997. Differences in active site gorge dimensions of cholinesterases revealed by binding of inhibitors to human butyrylcholinesterase. Biochemistry 36: 14642-14651.
34. Saxena A, Sun W, Dabisch PA, Hulet SW, Hastings NB, Jakubowski EM, Mioduszewski RJ, Doctor BP. 2011a. Pretreatment with human serum butyrylcholinesterase alone prevents cardiac abnormalities, seizures, and death in Göttingen minipigs exposed to sarin vapor. Biochem. Pharmacol. 82: 1984-1993.
35. Saxena A, Sun W, Fedorko JM, Koplovitz I, Doctor BP. 2011b. Prophylaxis with human serum butyrylcholinesterase protects guinea pigs exposed to multiple lethal doses of soman or VX. Biochem. Pharmacol. 81: 164-169.
36. Shih TM, Kan RK, McDonough JH. 2005. In vivo cholinesterase inhibitory specificity of organophosphorus nerve agents. Chem. Biol. Interact. 157-158: 293-303; doi: 10.1016/j.cbi.2005.10.042.
37. Sun W, Doctor BP, Lenz DE, Saxena A. 2008. Long-term effects of human butyrylcholinesterase pretreatment followed by acute soman challenge in cynomolgus monkeys. Chem. Biol. Interact. 175: 428-430; doi: 10.1016/j.cbi.2008.04.036.
38. Sun W, Luo C, Naik RS, Doctor BP, Saxena A. 2009. Pharmacokinetics and immunologic consequences of repeated administrations of purified heterologous and homologous butyrylcholinesterase in mice. Life Sci. 85: 657-661.
39. Tecles F, Ceron JJ. 2001. Determination of whole blood cholinesterase in different animal species using specific substrates. Res. Vet. Sci. 70: 233-238; doi: 10.1053/rvsc.2001.0465.
40. Van Kampen E, Zijlstra WG. 1961. Standardization of hemoglobinometry. II. The hemiglobincyanide method. Clin. Chim. Acta 6: 538-544.
41. Vellom DC, Radic Z, Li Y, Pickering NA, Camp S, Taylor P. 1993. Amino acid residues controlling acetylcholinesterase and butyrylcholinesterase specificity. Biochemistry 32: 12-17.
42. Wagner GM, Chiu DT, Qju JH, Heath RH, Lubin BH. 1987. Spectrin oxidation correlates with membrane vesiculation in stored RBCs. Blood 69: 1777-1981.
43. Wilson BW, Arrieta DE, Henderson JD. 2005. Monitoring cholinesterases to detect pesticide exposure. Chem. Biol. Interact. 157-158: 253-256; doi: 10.1016/j.cbi.2005.10.043.
44. Worek F, Mast U, Kiderlen D, Diepold C, Eyer P. 1999. Improved determination of acetylcholinesterase activity in human whole blood. Clin. Chim. Acta 288: 73-90; doi: 10.1016/S0009-8981(99)00144-8.