Doublets pretending to be CD34+ T cells despite doublet exclusion

Cytometry Part A

Volumn 83 A, Issue 2, 2013, Pages 173-176

  Kudernatsch, Robert Friedrich ^a   Letsch, Anne ^a   Stachelscheid, Harald ^a   Volk, Hans Dieter ^a   Scheibenbogen, Carmen ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CD3 ANTIGEN; CD34 ANTIGEN; DNA; FLUORESCENT DYE;

ARTICLE; ARTIFACT; CELL SEPARATION; CHEMISTRY; CYTOLOGY; FLOW CYTOMETRY; HUMAN; METABOLISM; STAINING; T LYMPHOCYTE;

ANTIGENS, CD3; ANTIGENS, CD34; ARTIFACTS; CELL SEPARATION; DNA; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; STAINING AND LABELING; T-LYMPHOCYTES;

EID: 84873096424     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22247     Document Type: Article

References (6)

1. Donnenberg AD, Donnenberg VS. Rare-event analysis in flow cytometry. Clin Lab Med 2007; 27: 627-652, viii.
2. Zimmerlin L, Donnenberg VS, Donnenberg AD. Rare event detection and analysis in flow cytometry: Bone marrow mesenchymal stem cells, breast cancer stem/progenitor cells in malignant effusions, and pericytes in disaggregated adipose tissue. Methods Mol Biol 2011; 699: 251-273.
3. Kuonen F, Touvrey C, Laurent J, Ruegg C. Fc block treatment, dead cells exclusion, and cell aggregates discrimination concur to prevent phenotypical artifacts in the analysis of subpopulations of tumor-infiltrating CD11b(+) myelomonocytic cells. Cytometry A 2010; 77A: 1082-1090.
4. Zhang Y, Joe G, Hexner E, Zhu J, Emerson SG. Host-reactive CD8+ memory stem cells in graft-versus-host disease. Nat Med 2005; 11: 1299-1305.
5. Wersto RP, Chrest FJ, Leary JF, Morris C, Stetler-Stevenson MA, Gabrielson E. Doublet discrimination in DNA cell-cycle analysis. Cytometry 2001; 46: 296-306.
6. van de Geijn G-J, van Rees V, van Pul-Bom N, Birnie E, Janssen H, Pegels H, Beunis M, Njo T. Leukoflow: Multiparameter extended white blood cell differentiation for routine analysis by flow cytometry. Cytometry A 2011; 79A: 694-706.