Fc block treatment, dead cells exclusion, and cell aggregates discrimination concur to prevent phenotypical artifacts in the analysis of subpopulations of tumor-infiltrating CD11b+ myelomonocytic cells

Cytometry Part A

Volumn 77, Issue 11, 2010, Pages 1082-1090

  Kuonen, Francois ^a,b   Touvrey, Cedric ^a   Laurent, Julien ^a   Ruegg, Curzio ^a,b  

^a LAUSANNE UNIVERSITY HOSPITAL   (Switzerland)

^b EPFL   (Switzerland)

Author keywords

4T1 tumor; CD11b; Flow cytometry; Myeloid cells; Tumor infiltrating leukocytes

Indexed keywords

CD11 ANTIGEN; FC RECEPTOR;

ANIMAL; ARTICLE; ARTIFACT; BAGG ALBINO MOUSE; BONE MARROW CELL; CELL AGGREGATION; CELL DEATH; CELL SEPARATION; EXPERIMENTAL NEOPLASM; FEMALE; FLOW CYTOMETRY; METABOLISM; METHODOLOGY; MONOCYTE; MOUSE; PATHOLOGY; TUMOR ASSOCIATED LEUKOCYTE; TUMOR CELL LINE;

ANIMALS; ANTIGENS, CD11; ARTIFACTS; BONE MARROW CELLS; CELL AGGREGATION; CELL DEATH; CELL LINE, TUMOR; CELL SEPARATION; FEMALE; FLOW CYTOMETRY; LYMPHOCYTES, TUMOR-INFILTRATING; MAMMARY NEOPLASMS, EXPERIMENTAL; MICE; MICE, INBRED BALB C; MONOCYTES; RECEPTORS, FC;

EID: 77958570971     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20969     Document Type: Article

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