Production of polyomavirus-like particles in a KLGAL80 knockout strain of the yeast kluyveromyces lactis

Preparative Biochemistry and Biotechnology

Volumn 43, Issue 2, 2013, Pages 217-235

  Simon, Claudia ^a   Schaepe, Sebastian ^a   Breunig, Karin ^a   Lilie, Hauke ^a  

^a MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

Author keywords

fermentation; KLGAl80; Kluyveromyces lactis; polyoma VP1; recombinant protein expression; VLP; yeast

Indexed keywords

BETA GALACTOSIDASE; CAPSID PROTEIN; FUNGAL PROTEIN; RECOMBINANT PROTEIN; REPRESSOR PROTEIN; VP1 PROTEIN, POLYOMAVIRUS;

ARTICLE; ENZYME ACTIVATION; FERMENTATION; GENE INACTIVATION; GENE LOCUS; GENE VECTOR; GENETICS; KLUYVEROMYCES; METABOLISM; MOLECULAR CLONING; POLYOMA VIRUS; PROTEIN DEGRADATION; PROTEIN STABILITY;

BETA-GALACTOSIDASE; CAPSID PROTEINS; CLONING, MOLECULAR; ENZYME ACTIVATION; FERMENTATION; FUNGAL PROTEINS; GENE KNOCKOUT TECHNIQUES; GENETIC LOCI; GENETIC VECTORS; KLUYVEROMYCES; POLYOMAVIRUS; PROTEIN STABILITY; PROTEOLYSIS; RECOMBINANT PROTEINS; REPRESSOR PROTEINS;

EUKARYOTA; KLUYVEROMYCES LACTIS; MURINE POLYOMAVIRUS; POLYOMAVIRUS;

EID: 84872966821     PISSN: 10826068     EISSN: 15322297     Source Type: Journal    
DOI: 10.1080/10826068.2012.750613     Document Type: Article

References (23)

1. Leavitt, A.D.; Roberts, T.M.; Garcea, R.L. Polyoma Virus Major Capsid Protein, VP1. Purification After High Level Expression in Escherichia coli. J. Biol. Chem. 1985, 260(23), 12803-12809. (Pubitemid 16230575)
2. Liew, M.W.; Rajendran, A.; Middelberg, A.P. Microbial Production of Virus-Like Particle Vaccine Protein at Gram-per-litre Levels. J. Biotechnol. 2010, 150(2), 224-231.
3. Sasnauskas, K.; Bulavaite, A.; Hale, A.; Jin, L.; Knowles, W.A.; Gedvilaite, A.; Dargeviciute, A.; Bartkeviciute, D.; Zvirbliene, A.; Staniulis, J.; et al. Generation of recombinant Virus-Like Particles of Human and Non-Human Polyomaviruses in Yeast Saccharomyces cerevisiae. Intervirology 2002, 45(4-6), 308-317. (Pubitemid 36173440)
4. Montross, L.; Watkins, S.; Moreland, R.B.; Mamon, H.; Caspar, D.L.; Garcea, R.L. Nuclear Assembly of Polyomavirus Capsids in Insect Cells Expressing the Major Capsid Protein VP1. J. Virol. 1991, 65(9), 4991-4998.
5. Salunke, D.M.; Caspar, D.L.; Garcea, R.L. Polymorphism in the Assembly of Polyomavirus Capsid Protein VP1. Biophys. J. 1989, 56(5), 887-900.
6. Palkova, Z.; Adamec, T.; Liebl, D.; Stokrova, J.; Forstova, J. Production of Polyomavirus Structural Protein VP1 in Yeast Cells and Its Interaction With Cell Structures. FEBS Lett. 2000, 478(3), 281-289. (Pubitemid 30621632)
7. Sasnauskas, K.; Buzaite, O.; Vogel, F.; Jandrig, B.; Razanskas, R.; Staniulis, J.; Scherneck, S.; Kruger, D.H.; Ulrich, R. Yeast Cells Allow High-Level Expression and Formation of Polyomavirus-Like Particles. Biol. Chem. 1999, 380(3), 381-386. (Pubitemid 29145952)
8. May, T.; Gleiter, S.; Lilie, H. Assessment of Cell Type Specific Gene Transfer of Polyoma Virus Like Particles Presenting a Tumor Specific Antibody Fv Fragment. J. Virol. Methods 2002, 105(1), 147-157.
9. Wang, T.; Zhang, Z.; Gao, D.; Li, F.; Wei, H.; Liang, X.; Cui, Z.; Zhang, X.E. Encapsulation of Gold Nanoparticles by Simian Virus 40 Capsids. Nanoscale 2011, 3(10), 4275-4282.
10. Li, F.; Li, K.; Cui, Z.Q.; Zhang, Z.P.; Wei, H.P.; Gao, D.; Deng, J.Y.; Zhang, X.E. Viral Coat Proteins as Flexible Nano-Building-Blocks for Nanoparticle Encapsulation. Small 2010, 6(20), 2301-2308.
11. Tegerstedt, K.; Franzen, A.V.; Andreasson, K.; Joneberg, J.; Heidari, S.; Ramqvist, T.; Dalianis, T. Murine Polyomavirus Virus-Like Particles (VLPs) as Vectors for Gene and Immune Therapy and Vaccines Against Viral Infections and Cancer. Anticancer Res. 2005, 25(4), 2601-2608. (Pubitemid 41044635)
12. van den Berg, J.A.; van der Laken, K.J.; van Ooyen, A.J.; Renniers, T.C.; Rietveld, K.; Schaap, A.; Brake, A.J.; Bishop, R.J.; Schultz, K.; Moyer, D.; et al. Kluyveromyces as a Host for Heterologous Gene Expression: Expression and Secretion of Prochymosin. Biotechnology (NY) 1990, 8(2), 135-139.
13. Breunig, K.D.; Kuger, P. Functional Homology Between the Yeast Regulatory Proteins GAL4 and LAC9: LAC9-Mediated Transcriptional Activation in Kluyveromyces lactis involves Protein Binding to a Regulatory Sequence Homologous to the GAL4 Protein-Binding Site. Mol. Cell Biol. 1987, 7(12), 4400-4406.
14. van Ooyen, A.J.; Dekker, P.; Huang, M.; Olsthoorn, M.M.; Jacobs, D.I.; Colussi, P.A.; Taron, C.H. Heterologous protein production in the yeast Kluyveromyces lactis. FEMS Yeast Res. 2006, 6(3), 381-392.
15. Krijger, J.J.; Baumann, J.; Wagner, M.; Schulze, K.; Reinsch, C.; Klose, T.; Onuma, O.F.; Simon, C.; Behrens, S.E.; Breunig, K.D. A novel, Lactase-Based Selection and Strain Improvement Strategy for Recombinant Protein Expression in Kluyveromyces lactis. Microb. Cell Fact. 2012, 11(1), 112.
16. Krijger, J.-J.; Baumann, J.; Wagner, M.; Schulze, K.; Reinsch, C.; Klose, T.; Onuma, O.F.; Simon, C.; Behrens, S.E.; Breunig, K.D. A Novel, Lactase-Based Selection and Strain Improvement Strategy for Recombinant Protein Expression in Kluyveromyces lactis. Microb. Cell Fact. 2012, 11(112), 1-12.
17. Zenke, F.T.; Zachariae, W.; Lunkes, A.; Breunig, K.D. Gal80 Proteins of Kluyveromyces lactis and Saccharomyces cerevisiae Are Highly Conserved but Contribute Differently to Glucose Repression of the Galactose Regulon. Mol. Cell Biol. 1993, 13(12), 7566-7576. (Pubitemid 23354281)
18. Das, S.; Hollenberg, C.P. A High-Frequency Transformation System for the Yeast Kluyveromyces lactis. Curr. Genet. 1982, 6, 123-128.
19. Barba, D.B.F.; Del Re, G.; Di Giacomo, G.; Veglio, F. Kinetic Analysis of Kluyveromyces lactis Fermentation on Whey: Batch and Fed-Batch Operations. Process Biochem. 2001, 36, 6.
20. Haynes, J.I. 2nd; Chang, D.; Consigli, R.A. Mutations in the Putative Calcium-Binding Domain of Polyomavirus VP1 Affect Capsid Assembly. J. Virol. 1993, 67(5), 2486-2495. (Pubitemid 23118842)
21. Adamec, T.; Palkova, Z.; Velkova, K.; Stokrova, J.; Forstova, J. Point Mutation in Calcium-Binding Domain of Mouse Polyomavirus VP1 Protein Does Not Prevent Virus-Like Particle Formation, but Changes VP1 Interactions With Saccharomyces cerevisiae Cell Structures. FEMS Yeast Res. 2005, 5(4-5), 331-340. (Pubitemid 40187187)
22. Gedvilaite, A.; Zvirbliene, A.; Staniulis, J.; Sasnauskas, K.; Kruger, D.H.; Ulrich, R. Segments of Puumala Hantavirus Nucleocapsid Protein Inserted Into Chimeric Polyomavirus-Derived Virus-Like Particles Induce a Strong Immune Response in Mice. Viral Immunol. 2004, 17(1), 51-68. (Pubitemid 38338462)
23. Middelberg, A.P.; Rivera-Hernandez, T.; Wibowo, N.; Lua, L.H.; Fan, Y.; Magor, G.; Chang, C.; Chuan, Y.P.; Good, M.F.; Batzloff, M.R. A Microbial Platform for Rapid and Low-Cost Virus-Like Particle and capsomere Vaccines. Vaccine 2011, 29(41), 7154-7162.