Screening quality for Ca2+-activated potassium channel in IonWorks Quattro is greatly improved by using BAPTA-AM and ionomycin

Journal of Pharmacological and Toxicological Methods

Volumn 67, Issue 1, 2013, Pages 16-24

  Ido, Katsutoshi ^a,c   Ohwada, Tomohiko ^b   Yasutomi, Eriko ^b   Yoshinaga, Takashi ^c   Arai, Tohru ^c   Kato, Mitsuyasu ^a   Sawada, Kohei ^a,c  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

^c EISAI CO LTD   (Japan)

Author keywords

BAPTA; BK channel; DiCl DHAA; Ionomycin; IonWorks Quattro; KCa1.1; KCNMA1; NS1619

Indexed keywords

ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; IONOMYCIN; LARGE CONDUCTANCE CALCIUM ACTIVATED POTASSIUM CHANNEL; O,O' BIS(2 AMINOPHENYL)ETHYLENE GLYCOL N,N,N',N' TETRAACETIC ACID TETRAACETOXYMETHYL ESTER; UNCLASSIFIED DRUG; 1,2 BIS(2 AMINOPHENOXY)ETHANE N,N,N',N' TETRAACETIC ACID ACETOXYMETHYL ESTER; 1,2-BIS(2-AMINOPHENOXY)ETHANE N,N,N',N'-TETRAACETIC ACID ACETOXYMETHYL ESTER; CALCIUM IONOPHORE; CHELATING AGENT; DRUG DERIVATIVE; EGTAZIC ACID;

ARTICLE; AUTOMATION; CALCIUM CURRENT; CALCIUM SIGNALING; CHANNEL GATING; CONTROLLED STUDY; MOLECULAR STABILITY; PATCH CLAMP; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; SCREENING TEST; ANIMAL; CELL LINE; CHO CELL; CRICETULUS; DRUG EFFECT; DRUG SCREENING; HAMSTER; MEMBRANE POTENTIAL; METHODOLOGY; PHYSIOLOGY; STANDARD;

ANIMALS; CALCIUM IONOPHORES; CELL LINE; CHELATING AGENTS; CHO CELLS; CRICETINAE; CRICETULUS; DRUG EVALUATION, PRECLINICAL; EGTAZIC ACID; IONOMYCIN; LARGE-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNELS; MEMBRANE POTENTIALS; PATCH-CLAMP TECHNIQUES;

EID: 84872938538     PISSN: 10568719     EISSN: 1873488X     Source Type: Journal    
DOI: 10.1016/j.vascn.2012.10.005     Document Type: Article

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