Simple and inexpensive three-step rapid amplification of cDNA 5′ ends using 5′ phosphorylated primers

Analytical Biochemistry

Volumn 434, Issue 1, 2013, Pages 1-3

  Dallmeier, Kai ^a   Neyts, Johan ^a  

^a REGA INSTITUTE FOR MEDICAL RESEARCH   (Belgium)

Author keywords

Linker cloning; Rapid amplification of cDNA ends (RACE); RNA cloning; Tobacco acid pyrophosphatase; Transcriptional initiation site mapping

Indexed keywords

CLONE CELLS; CLONING; MAMMALS; MAPPING; VIRUSES;

AMPLIFICATION PROTOCOLS; GENE-SPECIFIC PRIMERS; MAMMALIAN CELLS; NON-CODING REGION; PYROPHOSPHATASES; RAPID AMPLIFICATION OF CDNA ENDS; SEQUENCE ANALYSIS; TRANSCRIPTIONAL INITIATION;

PHOSPHORYLATION;

CAPPED RNA; COMPLEMENTARY DNA; GENOMIC RNA; MESSENGER RNA; PRIMER DNA; RIBONUCLEASE A; VIRUS RNA;

ACCURACY; AMPLICON; ARTICLE; BACTERIOPHAGE T4; DNA LIBRARY; DNA SYNTHESIS; GENE AMPLIFICATION; MOLECULAR CLONING; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN PHOSPHORYLATION; REVERSE TRANSCRIPTION; RNA EXTRACTION; YELLOW FEVER FLAVIVIRUS;

EID: 84872863915     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.10.031     Document Type: Article

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