Determination of the capped site sequence of mRNA based on the detection of cap-dependent nucleotide addition using an anchor ligation method

DNA Research

Volumn 11, Issue 4, 2004, Pages 305-309

  Ohtake, Hideki ^a,b   Ohtoko, Kuniyo ^a   Ishimaru, Yoshihiro ^b   Kato, Seishi ^a  

^a NATIONAL REHABILITATION CENTER FOR PERSONS WITH DISABILITIES   (Japan)

^b SAITAMA UNIVERSITY   (Japan)

Author keywords

Anchor ligation; Cap; Full length cDNA; Transcription start site

Indexed keywords

CAPPED RNA; COMPLEMENTARY DNA; MESSENGER RNA;

ANCHOR LIGATION; ARTICLE; CELL LINE; GENETIC TRANSCRIPTION; GENOME; HUMAN; HUMAN CELL; METHODOLOGY; NUCLEOTIDE SEQUENCE; PIGMENT EPITHELIUM; POLYMERASE CHAIN REACTION; RNA SEQUENCE; TRANSCRIPTION INITIATION;

DEOXYGUANINE NUCLEOTIDES; DNA, COMPLEMENTARY; HUMANS; RNA CAPS; RNA, MESSENGER; RNA-DIRECTED DNA POLYMERASE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, NUCLEIC ACID; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC; TRANSCRIPTION INITIATION SITE;

EID: 11144326722     PISSN: 13402838     EISSN: None     Source Type: Journal    
DOI: 10.1093/dnares/11.4.305     Document Type: Article

References (13)

1. Okayama, H. and Berg, P. 1982, High-efficiency cloning of full-length cDNA, Mol. Cell. Biol., 2, 161-170.
2. Pruitt, S. C. 1988, Expression vectors permitting cDNA cloning and enrichment for specific sequences by hybridization/selection, Gene, 66, 121-134.
3. Frohman, M. A., Dush, M. K., and Martin, G. R. 1988, Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer, Proc. Natl. Acad. Sci. USA, 85, 8998-9002.
4. Edwards, J. B., Delort, J., and Mallet, J. 1991, Oligodeoxyribonucleotide ligation to single-stranded cDNAs: a new tool for cloning 5′ ends of mRNAs and for constructing cDNA libraries by in vitro amplification, Nucleic Acids Res., 19, 5227-5232.
5. Troutt, A. B., McHeyzer-Williams, M. G., Pulendran, B., and Nossal G. J. 1992, Ligation-anchored PCR: a simple amplification technique with single-sided specificity, Proc. Natl. Acad. Sci. USA, 89, 9823-9825.
6. Apte, A. N. and Siebert, P. D. 1993, Anchor-ligated cDNA libraries: a technique for generating a cDNA library for the immediate cloning of the 5′ ends of mRNAs, Biotechniques, 15, 890-893.
7. Carninci, P., Kvam, C., Kitamura, A. et al. 1996, High-efficiency full-length cDNA cloning by biotinylated CAP trapper, Genomics, 37, 327-336.
8. Maruyama, K. and Sugano, S. 1994, Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides, Gene, 138, 171-174.
9. Kato, S., Sekine, S., Oh, S.-W. et al. 1994, Construction of a human full-length cDNA bank, Gene, 150, 243-250.
10. Clontech Laboratories 1996, CLONTECniques, 11, 2-4.
11. Schmidt, W. M. and Mueller, M. W. 1999, CapSelect: a highly sensitive method for 5′ CAP-dependent enrichment of full-length cDNA in PCR-mediated analysis of mRNAs, Nucleic Acids Res., 27, e31.
12. Chen, D. and Patton, J. T. 2001, Reverse transcriptase adds nontemplated nucleotides to cDNAs during 5′-RACE and primer extension, Biotechniques, 30, 574-582.
13. Shibata, Y., Carninci, P., Watahiki, A. et al. 2001, Cloning full-length, cap-trapper-selected cDNAs by using the single-strand linker ligation method, Biotechniques, 30, 1250-1254.