Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC)

Optics Express

Volumn 21, Issue 1, 2013, Pages 1189-1202

  Elting, Mary Williard ^a,b   Leslie, Sabrina R ^c,d   Churchman, L Stirling ^a,e   Korlach, Jonas ^f   McFaul, Christopher M J ^c   Leith, Jason S ^c   Levene, Michael J ^g   Cohen, Adam E ^d   Spudich, James A ^a  

^a STANFORD UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c MCGILL UNIVERSITY   (Canada)

^d HARVARD UNIVERSITY   (United States)

^e HARVARD MEDICAL SCHOOL   (United States)

^f PACIFIC BIOSCIENCES   (United States)

^g Yale University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENCE; FLUOROPHORES; LINEAR MOTORS; MOLECULES; REFRACTIVE INDEX; WAVEGUIDES;

ACTIN FILAMENT; BACKGROUND SUPPRESSION; BIOLOGICAL INTERACTIONS; CHEMO-MECHANICAL COUPLINGS; CONVEX LENS; CYTOSKELETAL FILAMENTS; FILAMENTOUS TRACKS; FLUORESCENCE IMAGING; FLUORESCENT MOLECULES; FLUOROPHORE CONCENTRATION; KINESINS; MICROTUBULES; MOLECULAR MOTORS; MYOSIN V; SINGLE-MOLECULE; SINGLE-MOLECULE BIOPHYSICS; SINGLE-MOLECULE FLUORESCENCE MICROSCOPY; TIRF MICROSCOPY; TOTAL INTERNAL REFLECTION FLUORESCENCE;

PROTEINS;

ACTIN; ADENOSINE TRIPHOSPHATE; DYNEIN ADENOSINE TRIPHOSPHATASE; KINESIN; MYOSIN;

ANIMAL; ARTICLE; BIOPHYSICS; CHEMISTRY; COMPUTER SIMULATION; CYTOSKELETON; EQUIPMENT; EQUIPMENT DESIGN; FLUORESCENCE IMAGING; FLUORESCENCE MICROSCOPY; INSECT; METABOLISM; METHODOLOGY; MICROSCOPY; MICROTUBULE; OPTICAL INSTRUMENTATION; PHYSICS;

ACTINS; ADENOSINE TRIPHOSPHATE; ANIMALS; BIOPHYSICS; COMPUTER SIMULATION; CYTOSKELETON; DYNEINS; EQUIPMENT DESIGN; INSECTS; KINESIN; LENSES; MICROSCOPY; MICROSCOPY, FLUORESCENCE; MICROTUBULES; MYOSINS; OPTICAL IMAGING; PHYSICS;

EID: 84872713972     PISSN: None     EISSN: 10944087     Source Type: Journal    
DOI: 10.1364/OE.21.001189     Document Type: Article

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