Assays to measure nuclear mechanics in interphase cells

Current Protocols in Cell Biology

Volumn 1, Issue SUPPL.56, 2012, Pages

  Isermann, Philipp ^a   Davidson, Patricia M ^a   Sliz, Josiah D ^a   Lammerding, Jan ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Cell nuclear mechanics; Deformation; Microfluidics; Nucleus

Indexed keywords

ADHERENT CELL; ARTICLE; ASSAY; CELL CULTURE; CELL DENSITY; CELL NUCLEUS; CELL NUCLEUS MEMBRANE; CYTOSKELETON; EPITHELIUM CELL; EXTRACELLULAR MATRIX; HUMAN; INTERPHASE; MECHANICS; MICROFLUIDICS; MICROTECHNOLOGY; MYOBLAST; MYOTUBE; NONHUMAN; PERFUSION; PRIORITY JOURNAL; RIGIDITY; SKIN FIBROBLAST; BIOMECHANICS; BIOPHYSICS; CHEMISTRY; CULTURE TECHNIQUE; GENETICS; METHODOLOGY; MICROFLUIDIC ANALYSIS;

BIOMECHANICS; BIOPHYSICS; CELL CULTURE TECHNIQUES; CELL NUCLEUS; HUMANS; INTERPHASE; MICROFLUIDIC ANALYTICAL TECHNIQUES;

EID: 84872686992     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb2216s56     Document Type: Article

References (13)

1. Caille, N., Tardy, Y., and Meister, J.J. 1998. Assessment of strain field in endothelial cells subjected to uniaxial deformation of their substrate. Ann. Biomed. Eng. 26:409-416.
2. Dahl, K.N., Kahn, S.M.,Wilson, K.L., and Discher, D.E. 2004. The nuclear envelope lamina network has elasticity and a compressibility limit suggestive of a molecular shock absorber. J. Cell Sci. 117:4779-4786.
3. Friedl, P., Wolf, K., and Lammerding, J. 2010. Nuclear mechanics during cell migration. Curr. Opin. Cell Biol. 23:55-64.
4. Holaska, J.M. 2008. Emerin and the nuclear lamina in muscle and cardiac disease. Circ. Res. 103:16-23.
5. Lammerding, J., Schulze, P.C., Takahashi, T., Kozlov, S., Sullivan, T., Kamm, R.D., Stewart, C.L., and Lee, R.T. 2004. Lamin A/C deficiency causes defective nuclear mechanics and mechanotransduction. J. Clin. Invest. 113:370-378.
6. Lammerding, J., Dahl, K.N., Discher, D.E., and Kamm, R.D. 2007. Nuclear mechanics and methods. Methods Cell Biol. 83:269-294.
7. Lombardi, M.L. and Lammerding, J. 2011.Keeping the LINC: The importance of nucleocytoskeletal coupling in intracellular force transmission and cellular function. Biochem. Soc. Trans. 39:1729-1734.
8. Lombardi, M.L., Zwerger, M., and Lammerding, J. 2011. Biophysical assays to probe the mechanical properties of the interphase cell nucleus: Substrate strain application and microneedle manipulation. J. Vis. Exp. 55:e3087.
9. Pajerowski, J.D., Dahl, K.N., Zhong, F.L., Sammak, P.J., and Discher, D.E. 2007. Physical plasticity of the nucleus in stem cell differentiation. Proc. Natl. Acad. Sci. U.S.A. 104:15619-15624.
10. Rosenbluth, M.J., Lam, W.A., and Fletcher, D.A. 2008. Analyzing cell mechanics in hematologic diseases with microfluidic biophysical flow cytometry. Lab. Chip 8:1062-1070.
11. Rowat, A.C., Lammerding, J., Herrmann, H., and Aebi, U. 2008. Towards an integrated understanding of the structure and mechanics of the cell nucleus. BioEssays 30:226-236.
12. Simon, D.N. and Wilson, K.L. 2011. The nucleoskeleton as a genome-associated dynamic "network of networks". Nat. Rev. Mol. Cell Biol. 12:695-708.
13. Zwerger, M., Ho, C.Y., and Lammerding, J. 2011. Nuclear mechanics in disease. Annu. Rev. Biomed. Eng. 13:397-428.