Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling

Journal of Biomedical Materials Research - Part A

Volumn 101 A, Issue 2, 2013, Pages 340-348

  Baxter, Ruth M ^a   Dai, Tianhong ^b,c   Kimball, Jess ^d   Wang, Eugenia ^e   Hamblin, Michael R ^b,c,f   Wiesmann, William P ^a   McCarthy, Simon J ^d   Baker, Shenda M ^a  

^a Synedgen Inc   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

^d HemCon Medical Technologies Inc   (United States)

^e Advanced Genomic Technology LLC   (United States)

^f HARVARD MIT DIVISION OF HEALTH SCIENCES AND TECHNOLOGY   (United States)

Author keywords

Biomarkers; Chitosan; Expression profiling; TGFb1; Wound healing

Indexed keywords

BIPHASIC EFFECTS; CHITOSAN TREATMENT; EXPRESSION PROFILING; GENE EXPRESSION ANALYSIS; GENE EXPRESSION PROFILING; MOUSE MODELS; MRNA LEVEL; QUANTITATIVE RT-PCR; SCAR FORMATION; TGFB1; TIME POINTS; TISSUE REGENERATION; WOUND HEALING;

BIOMARKERS; CHITOSAN; MAMMALS; TISSUE;

GENE EXPRESSION;

A DISINTEGRIN AND METALLOPEPTIDASE DOMAIN 9; ACETYL COENZYME A CARBOXYLASE BETA; ACONITASE 1; CHITOSAN; COILED COIL DOMAIN CONTAINING 47; COLLAGEN TYPE 3; DEP DOMAIN CONTAINING 5 PROTEIN; F BOX PROTEIN 38; GATA BINDING PROTEIN 1; KINESIN FAMILY MEMBER 3A; LACTIC ACID; MESSENGER RNA; NASCENT POLYPEPTIDE ASSOCIATED COMPLEX ALPHA POLYPEPTIDE; NICOTINAMIDE PHOSPHORIBOSYLTRANSFERASE; NOVAMATRIX P B; NOVAMATRIX UP B; PATERNALLY EXPRESSED 10 PROTEIN; PHOSPHORIBOSYL PYROPHOSPHATE SYNTHETASE 1; POLYURETHAN; PROTEIN; RGS2 PROTEIN; RIBOSEPHOSPHATE PYROPHOSPHOKINASE; RIPPLY3 HOMOLOG; SEPSECS PROTEIN; SORTING NEXIN; SORTING NEXIN 2; STRESS ACTIVATED PROTEIN KINASE 1; TAURINE UPREGULATED GENE 1 PROTEIN; TRANSFORMING GROWTH FACTOR BETA1; UBIQUITIN LIKE 5; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN; ZINC FINGER PROTEIN 280B;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BURN; CONTROLLED STUDY; DOWN REGULATION; FEMALE; FIBROSIS; GENE EXPRESSION PROFILING; GRANULATION TISSUE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCAR FORMATION; SIGNAL TRANSDUCTION; TISSUE PREPARATION; TISSUE REGENERATION; UPREGULATION; WOUND DRESSING; WOUND HEALING;

ANIMALS; BANDAGES; BURNS; CHITOSAN; DOWN-REGULATION; FEMALE; FIBROSIS; GENE EXPRESSION PROFILING; GENE REGULATORY NETWORKS; MICE; MICE, INBRED BALB C; REGENERATION; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER; SIGNAL TRANSDUCTION; UP-REGULATION; WOUND HEALING;

EID: 84872674466     PISSN: 15493296     EISSN: 15524965     Source Type: Journal    
DOI: 10.1002/jbm.a.34328     Document Type: Article

References (53)

1. Khor E. Chitin: Fulfilling a Biomaterials Promise. Amsterdam/New York: Elsevier Science Ltd.; 2001. Vol. xi, 136 pp.
2. Roberts GAF. Chitin Chemistry. London: Macmillan; 1992. Vol. xvi, 350 pp.
3. Baldrick P. The safety of chitosan as a pharmaceutical excipient. Regul Toxicol Pharmacol 2010; 56: 290-299.
4. Malette WG, Quigley HJ, Gaines R.D., Johnson ND, Rainer WG Chitosan: A new hemostatic. Ann Thorac Surg 1983; 36: 55-58.
5. Malmquist JP, Clemens SC, Oien H.J., Wilson SL Hemostasis of oral surgery wounds with the HemCon Dental Dressing. J Oral Maxillofac Surg 2008; 66: 1177-1183.
6. Pusateri AE, McCarthy SJ, Gregory K.W., Harris RA, Cardenas L, McManus AT, Goodwin CW Jr. Effect of a chitosan-based hemostatic dressing on blood loss and survival in a model of severe venous hemorrhage and hepatic injury in swine. J Trauma 2003; 54: 177-182.
7. Wedmore I, McManus JG, Pusateri A.E., Holcomb JB A special report on the chitosan-based hemostatic dressing: Experience in current combat operations. The Journal of trauma 2006; 60: 655-658.
8. Xie H, Teach JS, Burke A.P., Lucchesi LD, Wu PC, Sarao RC Laparoscopic repair of inferior vena caval injury using a chitosanbased hemostatic dressing. Am J surg 2009; 197: 510-514.
9. Burkatovskaya M, Castano AP, Demidova-Rice TN, Tegos G.P., Hamblin MR Effect of chitosan acetate bandage on wound healing in infected and noninfected wounds in mice. Wound Repair Regen 2008; 16: 425-431.
10. Burkatovskaya M, Tegos GP, Swietlik E, Demidova T.N., A PC, Hamblin MR Use of chitosan bandage to prevent fatal infections developing from highly contaminated wounds in mice. Biomaterials 2006; 27: 4157-4164.
11. Dai T, Tanaka M, Huang Y.Y., Hamblin MR Chitosan preparations for wounds and burns: Antimicrobial and wound-healing effects. Expert Rev Anti Infect Ther 2011; 9: 857-879.
12. Boucard N, Viton C, Agay D., Mari E, Roger T, Chancerelle Y., Domard A. The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials 2007; 28: 3478-3488.
13. Cho YW, Cho YN, Chung S.H., Yoo G., Ko SW Water-soluble chitin as a wound healing accelerator. Biomaterials 1999; 20: 2139-2145.
14. Prudden JF, Migel P, Hanson P., Friedrich L, Balassa L. The discovery of a potent pure chemical wound-healing accelerator. Am J Surg 1970; 119: 560-564.
15. Alsarra IA. Chitosan topical gel formulation in the management of burn wounds. Int J Biol Macromol 2009; 45: 16-21.
16. Kordestani S, Shahrezaee M, Tahmasebi M.N., Hajimahmodi H., Haji Ghasemali D, Abyaneh MS A randomised controlled trial on the effectiveness of an advanced wound dressing used in Iran. J Wound Care 2008; 17: 323-327.
17. Azad AK, Sermsintham N, Chandrkrachang S., Stevens WF Chitosan membrane as a wound-healing dressing: Characterization and clinical application. J Biomed Mater Res Part B Appl Biomater 2004; 69: 216-222.
18. Stone CA, Wright H, Clarke T., Powell R, Devaraj VS Healing at skin graft donor sites dressed with chitosan. Br J Plast Surg 2000; 53: 601-606.
19. Kim JH, Choi SJ, Park J.S., Lim KT, Choung PH, Kim SW, Lee J.B., Chung JH, Choung YH Tympanic membrane regeneration using a water-soluble chitosan patch. Tissue Eng Part A 2010; 16: 225-232.
20. Kojima K, Okamoto Y, Miyatake K., Fujise H, Shigemasa Y, Minami S. Effects of chitin and chitosan on collagen synthesis in wound healing. J Veterinary Med Sci 2004; 66: 1595-1598.
21. Ueno H, Nakamura F, Murakami M., Okumura M, Kadosawa T, Fujinag T. Evaluation effects of chitosan for the extracellular matrix production by fibroblasts and the growth factors production by macrophages. Biomaterials 2001; 22: 2125-2130.
22. Athanasiadis T, Beule AG, Robinson B.H., Robinson SR, Shi Z, Wormald PJ Effects of a novel chitosan gel on mucosal wound healing following endoscopic sinus surgery in a sheep model of chronic rhinosinusitis. Laryngoscope 2008; 118: 1088-1094.
23. Dai T, Tegos GP, Burkatovskaya M, Castano A.P., Hamblin MR Chitosan acetate bandage as a topical antimicrobial dressing for infected burns. Antimicrob Agents Chemother 2009; 53: 393-400.
24. Berscht PC, Nies B, Liebendorfer A., Kreuter J. In-vitro evaluation of biocompatibility of different wound dressing materials. J Mater Sci Mater Med 1995; 6: 201-205.
25. Khan TA, Peh KK, Ch'ng HS. Mechanical, bioadhesive strength and biological evaluations of chitosan films for wound dressing. J Pharm Pharm Sci 2000; 3: 303-311.
26. Park CJ, Clark SG, Lichtensteiger C.A., Jamison RD, Johnson AJW. Accelerated wound closure of pressure ulcers in aged mice by chitosan scaffolds with and without bFGF. Acta Biomater 2009; 5: 1926-1936.
27. Hong JP, Kim YW, Lee S.K., Kim SH, Min KH The effect of continuous release of recombinant human epidermal growth factor (rhEGF) in chitosan film on full thickness excisional porcine wounds. Ann Plast Surg 2008; 61: 457-462.
28. Baxter A, Dillon M, Taylor K.D., Roberts GA Improved method for i.r. determination of the degree of N-acetylation of chitosan. Int J Biol Macromol 1992; 14: 166-169.
29. Miya M, Iwamoto R, Yoshikawa S., Mima S. IR spectroscopic determination of conh content in highly deacylated chitosan. Int J Biol Macromol 1980; 2: 323-324.
30. Walker M, Hobot JA, Newman G.R., Bowler PG Scanning electron microscopic examination of bacterial immobilisation in a carboxymethyl cellulose (AQUACEL((R))) and alginate dressings. Biomaterials 2003; 24: 883-890.
31. Wheeler DL, Church DM, Federhen S, Lash A.E., Madden TL, Pontius JU, Schuler GD, Schriml L.M., Sequeira E., Tatusova TA and others. Database resources of the National Center for Biotechnology. Nucleic Acids Res 2003; 31: 28-33.
32. Tusher VG, Tibshirani R, Chu G. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci U S A 2001; 98: 5116-121.
33. Storey JD, Tibshirani R. Statistical significance for genomewide studies. Proc Natl Acad Sci U S A 2003; 100: 9440-9445.
34. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29: e45.
35. Huggett J, Dheda K, Bustin S., Zumla A. Real-time RT-PCR normalisation; strategies and considerations. Genes Immun 2005; 6: 279-284.
36. Lindblad WJ. Perspective article: Collagen expression by novel cell populations in the dermal wound environment. Wound Repair Regen 1998; 6: 186-193.
37. Holster T, Pakkanen O, Soininen R., Sormunen R, Nokelainen M, Kivirikko K.I., Myllyharju J. Loss of assembly of the main basement membrane collagen, type IV, but not fibril-forming collagens and embryonic death in collagen prolyl 4-hydroxylase I null mice. J Biol Chem 2007; 282: 2512-2519.
38. Gorlich D, Dabrowski M, Bischoff F.R., Kutay U., Bork P, Hartmann E, Prehn S., Izaurralde E. A novel class of RanGTP binding proteins. J Cell Biol 1997; 138: 65-80.
39. Nagata Y, Oda M, Nakata H., Shozaki Y, Kozasa T, Todokoro K. A novel regulator of G-protein signaling bearing GAP activity for Galphai and Galphaq in megakaryocytes. Blood 2001; 97: 3051-3060.
40. Sherr CJ. D-type cyclins. Trends Biochem Sci 1995; 20: 187-190.
41. White JM. ADAMs: Modulators of cell-cell and cell-matrix interactions. Current Opin Cell Biol 2003; 15: 598-606.
42. Song B, Haycraft CJ, Seo H.S., Yoder BK, Serra R. Development of the post-natal growth plate requires intraflagellar transport proteins. Dev Biol 2007; 305: 202-216.
43. Bhogal RK, Stoica CM, McGaha T.L., Bona CA Molecular aspects of regulation of collagen gene expression in fibrosis. J Clin Immunol 2005; 25: 592-603.
44. Sisson TH, Simon RH The plasminogen activation system in lung disease. Curr Drug Targets 2007; 8: 1016-1029.
45. Demirtas Y, Yagmur C, Soylemez F., Ozturk N, Demir A. Management of split-thickness skin graft donor site: A prospective clinical trial for comparison of five different dressing materials. Burns 2010; 36: 999-1005.
46. Saba SC, Tsai R, Glat P. Clinical evaluation comparing the efficacy of aquacel ag hydrofiber dressing versus petrolatum gauze with antibiotic ointment in partial-thickness burns in a pediatric burn center. J Burn Care Res 2009; 30: 380-385.
47. Lauper N, Beck AR, Cariou S, Richman L., Hofmann K, Reith W, Slingerland J.M., Amati B. Cyclin E2: A novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle. Oncogene 1998; 17: 2637-2643.
48. Thomas FC, Sheth B, Eckert J.J., Bazzoni G., Dejana E, Fleming TP Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo. J Cell Sci 2004; 117(Pt 23): 5599-5608.
49. Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 2002; 298: 1911-1912.
50. Alcorn JF, van der Velden J, Brown AL, McElhinney B, Irvin CG, Janssen-Heininger YM. C-Jun N-terminal kinase 1 is required for the development of pulmonary fibrosis. Am J Respir Cell Mol Biol 2009; 40: 422-432.
51. Rhett JM, Ghatnekar GS, Palatinus J.A., O'Quinn M, Yost MJ, Gourdie RG Novel therapies for scar reduction and regenerative healing of skin wounds. Trends Biotechnol 2008; 26: 173-180.
52. Liu W, Wang DR, Cao YL TGF-beta: A fibrotic factor in wound scarring and a potential target for anti-scarring gene therapy. Curr Gene Ther 2004; 4: 123-136.
53. Singer AJ, Clark RA Cutaneous wound healing. N Engl J Med 1999; 341: 738-746.