A heparin-based method for flow cytometric analysis of microparticles directly from platelet-poor plasma in calcium containing buffer

Journal of Immunological Methods

Volumn 388, Issue 1-2, 2013, Pages 49-59

  Iversen, Line V ^a,b   Østergaard, Ole ^b   Nielsen, Christoffer T ^b   Jacobsen, Søren ^c   Heegaard, Niels H H ^b  

^a BISPEBJERG HOSPITAL   (Denmark)

^b STATENS SERUM INSTITUT   (Denmark)

^c University of Copenhagen   (Denmark)

Author keywords

Annexin V binding; AnxV; AnxV+; AnxV ; APC; CD; EMPs; FACS; FITC; Flow cytometry method; FSC; HCs; Heparin; LMPs; Microparticles; MPs; PBS; PE; PFP; Phosphatidylserine; Platelet poor plasma; PMPs; PPP; PS; SLE; SSC

Indexed keywords

BUFFER; CALCIUM; CALCIUM ION; HEPARIN; LIPOCORTIN 5; MICROPARTICLE; NANOPARTICLE; UNCLASSIFIED DRUG;

ADULT; ARTICLE; BLOOD CLOTTING; BLOOD SAMPLING; CLINICAL ARTICLE; CONTROLLED STUDY; FEMALE; FLOW CYTOMETRY; FREEZING; HEPARINIZATION; HUMAN; INTERMETHOD COMPARISON; MALE; PRIORITY JOURNAL; PROTEIN BINDING; SYSTEMIC LUPUS ERYTHEMATOSUS; THROMBOCYTE POOR PLASMA;

BLOOD PLATELETS; CALCIUM; CELL-DERIVED MICROPARTICLES; FEMALE; FLOW CYTOMETRY; HEPARIN; HUMANS; LUPUS ERYTHEMATOSUS, SYSTEMIC; MALE; MIDDLE AGED; REPRODUCIBILITY OF RESULTS; STATISTICS, NONPARAMETRIC;

EID: 84872601399     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2012.12.001     Document Type: Article

References (33)

1. Amabile N., Guerin A.P., Leroyer A., Mallat Z., Nguyen C., Boddaert J., London G.M., Tedgui A., Boulanger C.M. Circulating endothelial microparticles are associated with vascular dysfunction in patients with end-stage renal failure. J. Am. Soc. Nephrol. 2005, 16:3381.
2. Ayers L., Kohler M., Harrison P., Sargent I., Dragovic R., Schaap M., Nieuwland R., Brooks S.A., Ferry B. Measurement of circulating cell-derived microparticles by flow cytometry: sources of variability within the assay. Thromb. Res. 2011, 127:370.
3. Berckmans R.J., Nieuwland R., Boing A.N., Romijn F.P., Hack C.E., Sturk A. Cell-derived microparticles circulate in healthy humans and support low grade thrombin generation. Thromb. Haemost. 2001, 85:639.
4. Bevers E.M., Comfurius P., Dekkers D.W., Harmsma M., Zwaal R.F. Regulatory mechanisms of transmembrane phospholipid distributions and pathophysiological implications of transbilayer lipid scrambling. Lupus 1998, 7(Suppl. 2:S126-31):S126.
5. Biró E., Nieuwland R., Tak P.P., Pronk L.M., Schaap M.C., Sturk A., Hack C.E. Activated complement components and complement activator molecules on the surface of cell-derived microparticles in patients with rheumatoid arthritis and healthy individuals. Ann. Rheum. Dis. 2007, 66:1085.
6. Cocucci E., Racchetti G., Meldolesi J. Shedding microvesicles: artefacts no more. Trends Cell Biol. 2009, 19:43.
7. Connor D.E., Exner T., Ma D.D., Joseph J.E. The majority of circulating platelet-derived microparticles fail to bind annexin V, lack phospholipid-dependent procoagulant activity and demonstrate greater expression of glycoprotein Ib. Thromb. Haemost. 2010, 103:1044.
8. Dey-Hazra E., Hertel B., Kirsch T., Woywodt A., Lovric S., Haller H., Haubitz M., Erdbruegger U. Detection of circulating microparticles by flow cytometry: influence of centrifugation, filtration of buffer, and freezing. Vasc. Health Risk Manag. 2010, 6(1125-33):1125.
9. Dignat-George F., Camoin-Jau L., Sabatier F., Arnoux D., Anfosso F., Bardin N., Veit V., Combes V., Gentile S., Moal V., Sanmarco M., Sampol J. Endothelial microparticles: a potential contribution to the thrombotic complications of the antiphospholipid syndrome. Thromb. Haemost. 2004, 91:667.
10. Distler J.H., Huber L.C., Gay S., Distler O., Pisetsky D.S. Microparticles as mediators of cellular cross-talk in inflammatory disease. Autoimmunity 2006, 39:683.
11. Enjeti A.K., Lincz L., Seldon M. Bio-maleimide as a generic stain for detection and quantitation of microparticles. Int. J. Lab. Hematol. 2008, 30:196.
12. Gao C., Boylan B., Fang J., Wilcox D.A., Newman D.K., Newman P.J. Heparin promotes platelet responsiveness by potentiating alphaIIbbeta3-mediated outside-in signaling. Blood 2011, 117:4946.
13. Heijnen H.F., Schiel A.E., Fijnheer R., Geuze H.J., Sixma J.J. Activated platelets release two types of membrane vesicles: microvesicles by shedding and exosomes derived from exocytosis of multivesicular bodies an alpha-granules. Blood 1999, 1:94(11):3791.
14. Hemker H.C., Beguin S. Mode of action of heparin and related drugs. Semin. Thromb. Hemost. 1991, 17(Suppl. 1:29-34):29.
15. Hochberg M.C. Updating the American College of Rheumatology revised criteria for the classification of systemic lupus erythematosus. Arthritis Rheum. 1997, 40:1725.
16. Horstman L.L., Jy W., Jimenez J.J., Ahn Y.S. Endothelial microparticles as markers of endothelial dysfunction. Front. Biosci. 2004, 9(1118-35):1118.
17. Jayachandran M., Miller V.M., Heit J.A., Owen W.G. Methodology for isolation, identification and characterization of microvesicles in peripheral blood. J. Immunol. Methods 2012, 375:207.
18. Jimenez J.J., Jy W., Mauro L.M., Horstman L.L., Ahn Y.S. Elevated endothelial microparticles in thrombotic thrombocytopenic purpura: findings from brain and renal microvascular cell culture and patients with active disease. Br. J. Haematol. 2001, 112:81.
19. Joop K., Berckmans R.J., Nieuwland R., Berkhout J., Romijn F.P., Hack C.E., Sturk A. Microparticles from patients with multiple organ dysfunction syndrome and sepsis support coagulation through multiple mechanisms. Thromb. Haemost. 2001, 85:810.
20. Jy W., Horstman L.L., Jimenez J.J., Ahn Y.S., Biró E., Nieuwland R., Sturk A., Dignat-George F., Sabatier F., Camoin-Jau L., Sampol J., Hugel B., Zobairi F., Freyssinet J.M., Nomura S., Shet A.S., Key N.S., Hebbel R.P. Measuring circulating cell-derived microparticles. J. Thromb. Haemost. 2004, 2:1842.
21. Lacroix R., Robert S., Poncelet P., Kasthuri R.S., Key N.S., Dignat-George F. Standardization of platelet-derived microparticle enumeration by flow cytometry with calibrated beads: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop. J. Thromb. Haemost. 2010, 8:2571.
22. Nielsen C.T., Ostergaard O., Johnsen C., Jacobsen S., Heegaard N.H. Distinct features of circulating microparticles and their relationship to clinical manifestations in systemic lupus erythematosus. Arthritis Rheum. 2011, 63:3067.
23. Nomura S. Function and clinical significance of platelet-derived microparticles. Int. J. Hematol. 2001, 74:397.
24. Perez-Pujol S., Marker P.H., Key N.S. Platelet microparticles are heterogeneous and highly dependent on the activation mechanism: studies using a new digital flow cytometer. Cytometry A 2007, 71:38.
25. Robert S., Poncelet P., Lacroix R., Arnaud L., Giraudo L., Hauchard A., Sampol J., Dignat-George F. Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: a first step towards multicenter studies?. J. Thromb. Haemost. 2009, 7:190.
26. Shah M.D., Bergeron A.L., Dong J.F., Lopez J.A. Flow cytometric measurement of microparticles: pitfalls and protocol modifications. Platelets 2008, 19:365.
27. Shet A.S., Aras O., Gupta K., Hass M.J., Rausch D.J., Saba N., Koopmeiners L., Key N.S., Hebbel R.P. Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes. Blood 2003, 102:2678.
28. Thiagarajan P., Tait J.F. Binding of annexin V/placental anticoagulant protein I to platelets. Evidence for phosphatidylserine exposure in the procoagulant response of activated platelets. J. Biol. Chem. 1990, 265:17420.
29. van der Heyde H.C., Gramaglia I., Combes V., George T.C., Grau G.E. Flow cytometric analysis of microparticles. Methods Mol. Biol. 2011, 699(337-54):337.
30. van der Pol E., van Gemert M.J.C., Sturk A., Nieuwland R., van Leeuwen T.G. Single vs swarm detection of microparticles and exosomes by flow cytometry. Thromb. Haemost. 2012, 10:919.
31. van Ierssel S.H., Van Craenenbroeck E.M., Conraads V.M., Van Tendeloo V.F., Vrints C.J., Jorens P.G., Hoymans V.Y. Flow cytometric detection of endothelial microparticles (EMP): effects of centrifugation and storage alter with the phenotype studied. Thromb. Res. 2010, 125:332.
32. Yuana Y., Bertina R.M., Osanto S. Pre-analytical and analytical issues in the analysis of blood microparticles. Thromb. Haemost. 2011, 105:396.
33. Zwicker J.I., Liebman H.A., Neuberg D., Lacroix R., Bauer K.A., Furie B.C., Furie B. Tumor-derived tissue factor-bearing microparticles are associated with venous thromboembolic events in malignancy. Clin. Cancer Res. 2009, 15:6830.