In-gel nonspecific proteolysis for elucidating glycoproteins: A method for targeted protein-specific glycosylation analysis in complex protein mixtures

Analytical Chemistry

Volumn 85, Issue 2, 2013, Pages 956-963

  Nwosu, Charles C ^a   Huang, Jincui ^a   Aldredge, Danielle L ^a   Strum, John S ^a   Hua, Serenus ^a   Seipert, Richard R ^a   Lebrilla, Carlito B ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURATE MASS; BOVINE MILK; GEL ELECTROPHORESIS; GLYCANS; GLYCOPEPTIDES; HUMAN SERUM; IN-HOUSE SOFTWARE; INGEL DIGESTION; LACTOFERRIN; N-GLYCOSYLATION; NANO-LC; NANOFLOWS; O-GLYCOSYLATION; PEPTIDE TAGS; POROUS GRAPHITIZED CARBONS; PROTEIN BANDS; PROTEIN IDENTIFICATION; PROTEIN MIXTURES; PROTEINASE K; QUADRUPOLE TIME OF FLIGHT MASS SPECTROMETRY; RAPID DETERMINATION; SITE-SPECIFIC; SUBTILISIN; TANDEM MASS SPECTROMETRY; TANDEM MS;

ELECTROPHORESIS; GLYCOPROTEINS; GLYCOSYLATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MIXTURES; PEPTIDES; PROTEOLYSIS; SEPARATION;

ESTERIFICATION;

GLYCOPROTEIN;

ARTICLE; CHEMISTRY; GEL; GLYCOSYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; GELS; GLYCOPROTEINS; GLYCOSYLATION; PROTEOLYSIS;

EID: 84872593638     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac302574f     Document Type: Article

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