Validation of IRS PCR, a molecular typing method, for the study of the diversity and population dynamics of Legionella in industrial cooling circuits

Letters in Applied Microbiology

Volumn 56, Issue 2, 2013, Pages 135-141

  Jakubek, D ^a,b   Le Brun, M ^a   Leblon, G ^b   Dubow, M ^b   Binet, M ^a  

^a EDF R AND D   (France)

^b INSTITUTE FOR INTEGRATIVE BIOLOGY OF THE CELL I2BC   (France)

Author keywords

Bacterial typing techniques; Infrequent restriction site PCR; Legionella; Pulsed field gel electrophoresis; Sequence based typing

Indexed keywords

COOLING; COOLING SYSTEMS; ECOLOGY; ELECTRIC NETWORK ANALYSIS; ELECTROPHORESIS; POLYMERASE CHAIN REACTION; THERMOELECTRIC POWER PLANTS; TIMING CIRCUITS;

AQUATIC ENVIRONMENTS; BACTERIAL TYPING; BACTERIAL TYPING TECHNIQUE; COOLING CIRCUITS; INFREQUENT RESTRICTION SITE PCR; LEGIONELLA PNEUMOPHILA; LEGIONELLAE; MOLECULAR TYPING; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE-BASED TYPING;

BACTERIA;

AQUATIC ENVIRONMENT; BACTERIAL DISEASE; BACTERIUM; ELECTROKINESIS; EPIDEMIOLOGY; POLYMERASE CHAIN REACTION; POPULATION DYNAMICS; SPECIES DIVERSITY;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; COOLING CIRCUIT; ECOLOGY; ELECTRIC POWER PLANT; ENVIRONMENTAL MONITORING; INFREQUENT RESTRICTION SITE POLYMERASE CHAIN REACTION; LEGIONELLA; LEGIONELLA PNEUMOPHILA; MOLECULAR TYPING; NONHUMAN; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; SEQUENCE BASED TYPING;

FRANCE;

BACTERIA (MICROORGANISMS); LEGIONELLA; LEGIONELLA PNEUMOPHILA;

EID: 84872397481     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/lam.12025     Document Type: Article

References (24)

1. Afshar, B., Fry, N.K., Bellamy, W., Underwood, A.P. and Harrison, T.G. (2007) External quality assessment of a DNA sequence-based scheme for epidemiological typing of Legionella pneumophila by an international network of laboratories. J Clin Microbiol 45, 3251-3256.
2. Association Française de Normalisation (2006) Qualité de l'eau - Recherche et dénombrement de Legionella spp et de Legionella pneumophila: Méthode par ensemencement direct et après concentration par filtration sur membrane ou centrifugation. NF T90-431.
3. Aurell, H., Farge, P., Meugnier, H., Gouy, M., Forey, F., Lina, G., Vandenesch, F., Etienne, J. et al. (2005) Clinical and environmental isolates of Legionella pneumophila serogroup 1 cannot be distinguished by sequence analysis of two surface protein genes and three housekeeping genes. Appl Environ Microbiol 71, 282-289.
4. Campese, C., Maine, C. and Che, D. (2010) Les cas de légionellose déclarés en France en 2009. Bulletin épidémiologique hebdomadaire 31-32, 334-335.
5. Casini, B., Valentini, P., Baggiani, A., Torracca, F., Lorenzini, C., Frateschi, S., Matteoli, B. and Privitera, G. (2008) Comparison of two molecular methods used for subtyping of Legionella pneumophila 1 strains isolated from a hospital water supply. Water Sci Technol 58, 683-688.
6. Che, D., Campèse, C. and Jarraud, S. (2009) Légionelles et légionellose: qu'a-t-on découvert depuis 30 ans? Pathol Biol (Paris) 59, 134-136.
7. De Zoysa, A.S. and Harrison, T.G. (1999) Molecular typing of Legionella pneumophila serogroup 1 by pulsed-field gel electrophoresis with Sfil and comparison of this method with restriction fragment-length polymorphism analysis. J Med Microbiol 48, 269-278.
8. Fry, N.K., Alexiou-Daniel, S., Bangsborg, J.M., Bernander, S., Pastoris, M.C., Etienne, J., Forsblom, B., Gaia, V. et al. (1999) A multicenter evaluation of genotypic methods for the epidemiologic typing of Legionella pneumophila serogroup 1: results of a pan-European study. Clin Microbiol Infect 5, 462-477.
9. Gaia, V., Fry, N.K., Harrison, T.G. and Peduzzi, R. (2003) Sequence-based typing of Legionella pneumophila serogroup 1 offers the potential for true portability in legionellosis outbreak investigation. J Clin Microbiol 41, 2932-2939.
10. Gaia, V., Fry, N.K., Afshar, B., Luck, P.C., Meugnier, H., Etienne, J., Peduzzi, R. and Harrison, T.G. (2005) Consensus sequence-based scheme for epidemiological typing of clinical and environmental isolates of Legionella pneumophila. J Clin Microbiol 43, 2047-2052.
11. Harrison, T.G., Doshi, N., Fry, N.K. and Joseph, C.A. (2007) Comparison of clinical and environmental isolates of Legionella pneumophila obtained in the UK over 19 years. Clin Microbiol Infect 13, 78-85.
12. Hunter, P.R. and Gaston, M.A. (1988) Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. J Clin Microbiol 26, 2465-2466.
13. Lawrence, C., Ronco, E., Dubrou, S., Leclercq, R., Nauciel, C. and Matsiota-Bernard, P. (1999) Molecular typing of Legionella pneumophila serogroup 1 isolates from patients and the nosocomial environment by arbitrarily primed PCR and pulsed-field gel electrophoresis. J Med Microbiol 48, 327-333.
14. Maes, N., Wauters, G. and Struelens, M.J. (1998) Evaluation of arbitrarily primed polymerase chain reaction analysis for typing Legionella pneumophila. Clin Microbiol Infect 4, 149-154.
15. Pruckler, J.M., Mermel, L.A., Benson, R.F., Giorgio, C., Cassiday, P.K., Breiman, R.F., Whitney, C.G. and Fields, B.S. (1995) Comparison of Legionella pneumophila isolates by arbitrarily primed PCR and pulsed-field gel electrophoresis: analysis from seven epidemic investigations. J Clin Microbiol 33, 2872-2875.
16. Ratzow, S., Gaia, V., Helbig, J.H., Fry, N.K. and Luck, P.C. (2007) Addition of neuA, the gene encoding N-acylneuraminate cytidylyltransferase, increases the discriminatory ability of the consensus sequence-based scheme for typing Legionella pneumophila serogroup 1 strains. J Clin Microbiol 45, 1965-1968.
17. Riffard, S., Lo Presti, F., Vandenesch, F., Forey, F., Reyrolle, M. and Etienne, J. (1998) Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains. J Clin Microbiol 36, 161-167.
18. Scaturro, M., Losardo, M., De Ponte, G. and Ricci, M.L. (2005) Comparison of three molecular methods used for subtyping of Legionella pneumophila strains isolated during an epidemic of Legionellosis in Rome. J Clin Microbiol 43, 5348-5350.
19. Schoonmaker, D., Heimberger, T. and Birkhead, G. (1992) Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak. J Clin Microbiol 30, 1491-1498.
20. Selander, R.K., McKinney, R.M., Whittam, T.S., Bibb, W.F., Brenner, D.J., Nolte, F.S. and Pattison, P.E. (1985) Genetic structure of populations of Legionella pneumophila. J Bacteriol 163, 1021-1037.
21. Simpson, E.H. (1949) Measurement of diversity. Nature 163, 688.
22. Struelens, M.J. (1996) Consensus guidelines for appropriate use and evaluation of microbial epidemiologic typing systems. Clin Microbiol Infect 2, 2-11.
23. Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24, 1596-1599.
24. Tenover, F.C., Arbeit, R.D., Goering, R.V., Mickelsen, P.A., Murray, B.E., Persing, D.H. and Swaminathan, B. (1995) Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33, 2233-2239.