Solvent selection and optimization of α-chymotrypsin-catalyzed synthesis of N-Ac-Phe-Tyr-NH2 using mixture design and response surface methodology

Biotechnology Progress

Volumn 28, Issue 6, 2012, Pages 1443-1449

  Hu, Shih Hao ^a   Kuo, Chia Hung ^b   Chang, Chieh Ming J ^b   Liu, Yung Chuan ^b   Chiang, Wen Dee ^a   Shieh, Chwen Jen ^b  

^a TUNGHAI UNIVERSITY   (Taiwan)

^b NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Chymotrypsin; Enzymatic peptide synthesis; N Ac Phe Tyr NH2; Organic aqueous solution

Indexed keywords

ANGIOTENSIN I CONVERTING ENZYME INHIBITOR; CENTRAL COMPOSITE ROTATABLE DESIGN; CHYMOTRYPSIN; ETHYL ESTERS; MIXTURE DESIGN; MOLAR RATIO; N-AC-PHE-TYR-NH2; OPTIMUM CONDITIONS; OPTIMUM REACTION; ORGANIC-AQUEOUS SOLVENTS; PEPTIDE SYNTHESIS; REACTION PARAMETERS; REACTION TEMPERATURE; RESPONSE SURFACE METHODOLOGY; SOLVENT SELECTION;

ACETONITRILE; AMINO ACIDS; BIOSYNTHESIS; CATALYSIS; CONDENSATION REACTIONS; OPTIMIZATION; PEPTIDES; SOLVENTS; SURFACE PROPERTIES;

ORGANIC SOLVENTS;

ACETONITRILE; ACETONITRILE DERIVATIVE; ALPHA-CHYMOTRYPSIN; CHYMOTRYPSIN; CHYMOTRYPSIN A; DIMETHYL SULFOXIDE; DIPEPTIDYL CARBOXYPEPTIDASE INHIBITOR; DRUG DERIVATIVE; L PHENYLALANINE ETHYLESTER; L-PHENYLALANINE ETHYLESTER; OLIGOPEPTIDE; PHENYLALANINE; SOLVENT; TROMETAMOL; TYROSINAMIDE; TYROSINE;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; CHEMISTRY; METABOLISM; METHODOLOGY; REGRESSION ANALYSIS; SYNTHESIS; TEMPERATURE;

ACETONITRILES; ANGIOTENSIN-CONVERTING ENZYME INHIBITORS; BIOREACTORS; CHYMOTRYPSIN; DIMETHYL SULFOXIDE; OLIGOPEPTIDES; PHENYLALANINE; REGRESSION ANALYSIS; RESEARCH DESIGN; SOLVENTS; TEMPERATURE; TROMETHAMINE; TYROSINE;

EID: 84872381489     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1623     Document Type: Article

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