DNA Barcode ITS Effectively Distinguishes the Medicinal Plant Boerhavia diffusa from Its Adulterants

Genomics, Proteomics and Bioinformatics

Volumn 10, Issue 6, 2012, Pages 364-367

  Selvaraj, Dhivya ^a   Shanmughanandhan, Dhivya ^a   Sarma, Rajeev Kumar ^a   Joseph, Jijo C ^a   Srinivasan, Ramachandran V ^a   Ramalingam, Sathishkumar ^a  

^a BHARATHIAR UNIVERSITY   (India)

Author keywords

Adulterant; Boerhavia diffusa; DNA barcoding; ITS; Punarnava

Indexed keywords

RIBOSOME DNA;

ARTICLE; BOERHAVIA DIFFUSA; BOERHAVIA ERECTA; BOERHAVIA REPANDA; BOERHAVIA VERTICILLATA; CHLOROPLAST GENE; GENE SEQUENCE; MEDICINAL PLANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; PHYLOGENY; PLASTID; PSBA TRNH GENE; SEQUENCE ALIGNMENT;

BASE SEQUENCE; CHLOROPLASTS; DNA BARCODING, TAXONOMIC; DNA, PLANT; DNA, RIBOSOMAL SPACER; INDIA; NYCTAGINACEAE; PHYLOGENY; PLANTS, MEDICINAL; SEQUENCE ALIGNMENT; SPECIES SPECIFICITY;

BOERHAVIA; BOERHAVIA DIFFUSA;

EID: 84872370039     PISSN: 16720229     EISSN: 22103244     Source Type: Journal    
DOI: 10.1016/j.gpb.2012.03.002     Document Type: Article

References (32)

1. Thakur R.S., Puri H.S., Hussain A. Major medicinal plants of India 1989, Central Institute of Medicinal and Aromatic Plants, Lucknow, p. 585.
2. Chaudhary G., Dantu P.K. Morphological, phytochemical and pharmacological, studies on Boerhavia diffusa L. J Med Plants Res 2011, 5:2125-2130.
3. Meena A.K., Niranjan U.S., Yadav A.K., Ajit K., Singh B., Kiran, et al. A quality assessment of Boerhavia diffusa Linn. Commonly known as "Punarnava" plant. Int J Pharmacog Phytochem Res 2010, 2:25-28.
4. Nalamolu R.K., Boini K.M., Nammi S. Effect of chronic administration of Boerhavia diffusa Linn. Leaf extract on experimental diabetes in rats. Trop J Pharm Res 2007, 3:305-309.
5. Rawat A.K.S., Mehrotra S., Tripathi S.C., Shome U. Hepatoprotective activity of Boerhavia diffusa L. roots-a popular Indian ethnomedicine. J Ethnopharmacol 1997, 56:61-66.
6. Olaleye M.T., Akinmoladun A.C., Ogunboye A.A., Akindahunsi A.A. Antioxidant activity and hepatoprotective property of leaf extracts of Boerhavia diffusa Linn against acetaminophen-induced liver damage in rats. Food Chem Toxicol 2010, 48:2200-2205.
7. Mudgal V. Studies on medicinal properties of Convolvulus pluricaulis and Boerhavia diffusa. Planta Med 1975, 28:62-68.
8. Satyavati G.V., Raina M.K., Sharma M. Medicinal plants of India 1976, Indian Council of Medical Research, New Delhi, p. 10-14.
9. Duhan A., Chauhan B.M., Punia D. Nutritional value of some non-conventional plant foods of India. Plant Food Hum Nutr 1992, 42:193-200.
10. Ujowundu C.O., Igwe C.U., Enemor V.H.A., Nwaogu L.A., Okafor O.E. Nutritive and anti-nutritive properties of Boerhavia diffusa and Commelia nudiflora leaves. Pak J Nutr 2008, 7:90-92.
11. Nandi R.P., Chatterjee S.K. Occurrence of Punarnavines in Boerhavia repens Linn. Indian J Exp Biol 1974, 12:509-511.
12. Awasthi L.P., Verma H.N. Boerhavia diffusa - a wild herb with potent biological and antimicrobial properties. Asian Agric Hist 2006, 10:55-68.
13. Porcher R.D. Boerhavia diffusa L. (B. coccinea Mill) (Nytaginaceae) in the Carolinas. Castanea 1978, 43:172-174.
14. Douglas N.A., Manos P.S. Molecular phylogeny of Nyctaginaceae: taxonomy, biogeography and characters associated with a radiation of xerophytic genera in North America. Am J Bot 2007, 94:856-872.
15. Bajpai A., Ojha J.K. Comparative studies of Boerhavia diffusa L. and Boerhavia verticillata poir (Nyctaginaceae). Anc Sci Life 2000, 19:105-109.
16. Balasubramani S.P., Murugan R., Ravikumar K., Venkatasubramanian P. Development of ITS sequence based molecular marker to distinguish, Tribulus terrestris L. (Zygophyllaceae) from its adulterant. Fitoterapia 2010, 81:503-508.
17. Bertini L., Amicucci A., Agostini D., Polidori E., Potenza L., Guidi C., et al. A new pair of primers designed for amplification of the ITS region in Tuber species. FEMS Microbiol Lett 1999, 173:239-245.
18. Song J., Yao H., Li Y., Li X., Lin Y., Liu C., et al. Authentication of the family Polygonaceae in Chinese pharmacopoeia by DNA barcoding techniques. J Ethnopharmacol 2009, 124:434-439.
19. Howard C., Bremner P.D., Fowler M.R., Isodo B., Scott N.W., Slater A. Molecular identification of Hypericum perforatum by PCR amplification of the ITS and 5.8S rDNA region. Planta Med 2009, 75:864-869.
20. Pereira F., Carneiro J., Amorim A. Identification of species with DNA-based technology: current progress and challenges. Recent Pat DNA Gene Seq 2008, 2:187-199.
21. Ferri G., Corradini B., Alu M. Capillary electrophoresis of multigene barcoding chloroplast markers for species identification of botanical trace evidence. Methods Mol Biol 2012, 830:253-263.
22. Gao T., Yao H., Song J., Liu C., Zhu Y., Ma X., et al. Identification of medicinal plants in the family Fabaceae using a potential DNA barcode ITS2. J Ethnopharmacol 2010, 130:116-121.
23. Youngbae S., Kim S., Park C.W. A phylogenetic study of Polygonum sect. Tovara (Polygonaceae) based on ITS sequences of nuclear ribosomal DNA. J Plant Biol 1977, 40:47-52.
24. Yao H., Song J.Y., Ma X.Y., Liu C., Li Y., Xu H.X., et al. Identification of Dendrobium species by a candidate DNA barcode sequence: the chloroplast psbA-trnH intergenic region. Planta Med 2009, 75:667-669.
25. Qiao C., Han Q., Zhao Z., Wang Z., Xu L., Xu H.X. Sequence analysis based on ITS1 region of nuclear ribosomal DNA of Amomum villosum and ten species of Alpinia. J Food Drug Anal 2009, 17:142-145.
26. Sass C., Little D.P., Stevenson D.W., Specht C.D. DNA barcoding in the Cycadales: testing the potential of proposed barcoding markers for species identification of cycads. PLoS One 2007, 2:e1154.
27. Al-Qurainy F., Khan S., Tarroum M., Al-Hemaid F.M., Ali M.A. Molecular authentication of the medicinal herb Ruta graveolens (Rutaceae) and an adulterant using nuclear and chloroplast DNA markers. Genet Mol Res 2011, 10:2806-2816.
28. Khanuja S.P., Shasany A.K., Darokar M.P., Kumar S. Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oil. Plant Mol Biol Rep 1999, 17:1-7.
29. White T.J., Bruns T.D., Lee S., Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications 1990, 315-322. Academic Press, New York. M.A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (Eds.).
30. Thompson J.D., Higgins D.G., Gibson T.J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994, 22:4673-4680.
31. Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007, 24:1596-1599.
32. Buchan I.E. The development of a statistical computer software resource for medical research 2000, University of Liverpool, Liverpool, UK.