The effect of pressure and mobile phase velocity on the retention properties of small analytes and large biomolecules in ultra-high pressure liquid chromatography

Journal of Chromatography A

Volumn 1270, Issue , 2012, Pages 127-138

  Fekete, Szabolcs ^a   Veuthey, Jean Luc ^a   McCalley, David V ^b   Guillarme, Davy ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b UNIVERSITY OF THE WEST OF ENGLAND   (United Kingdom)

Author keywords

Frictional heating; Pressure effect; Proteins; Retention; UHPLC; Ultra high pressure

Indexed keywords

ANALYTES; COMBINED EFFECT; CONFORMATIONAL CHANGE; EFFECT OF PRESSURE; ELEVATED PRESSURE; FRICTIONAL HEATING; GRADIENT ELUTION MODE; INCREASE IN PRESSURE; ISOCRATIC ELUTION; ISOCRATIC MODES; LARGE BIOMOLECULES; MOBILE PHASE FLOW RATE; MOBILE-PHASE VELOCITY; MOLECULAR SIZE; NUMEROUS MODELS; POST-COLUMN; RETENTION; RETENTION FACTORS; RETENTION PROPERTIES; SMALL MOLECULES; UHPLC; ULTRAHIGH PRESSURE;

CHROMATOGRAPHY; FLOW RATE; FRICTION; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MOLECULES; MONOCLONAL ANTIBODIES; PEPTIDES; PHASE VELOCITY; PROTEINS;

PRESSURE EFFECTS;

BEVACIZUMAB; BOVINE SERUM ALBUMIN; BUPIVACAINE; CYTOCHROME C; DITHIOTHREITOL; INSULIN; LIDOCAINE; MONOCLONAL ANTIBODY; MYOGLOBIN; PANITUMUMAB; PEPTIDE; PROPYL PARABEN; PROTEIN; RECOMBINANT GRANULOCYTE COLONY STIMULATING FACTOR; SALICYLIC ACID; TESTOSTERONE; TRIFLUOROACETIC ACID;

ARTICLE; CHEMICAL ANALYSIS; COLUMN CHROMATOGRAPHY; CONFORMATIONAL TRANSITION; ELUTION; FLOW RATE; MOBILE PHASE VELOCITY; MOLECULAR SIZE; MOLECULE; PHYSICAL PHENOMENA; PRESSURE; PRIORITY JOURNAL; RETENTION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; VELOCITY;

ANIMALS; ANTIBODIES, MONOCLONAL; CATTLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HORSES; HUMANS; MOLECULAR WEIGHT; PARTICLE SIZE; PEPTIDES; PRESSURE; PROTEIN CONFORMATION; PROTEINS;

EID: 84872264899     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.10.056     Document Type: Article

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