Isolation and molecular characterization of thirteen R2R3-MYB transcription factors from epimedium sagittatum

International Journal of Molecular Sciences

Volumn 14, Issue 1, 2013, Pages 594-610

  Huang, Wenjun ^a   Sun, Wei ^b   Lv, Haiyan ^a   Xiao, Gong ^a   Zeng, Shaohua ^b   Wang, Ying ^a  

^a Wuhan Institute of Botany Chinese Academy of Sciences   (China)

^b SOUTH CHINA INSTITUTE OF BOTANY   (China)

Author keywords

Epimedium; Flavonoid pathway; Medicinal plant; MYB; Transcription factor

Indexed keywords

COMPLEMENTARY DNA; FLAVONOID; MESSENGER RNA; PROTEIN MYB; TRANSCRIPTION FACTOR; VEGETABLE PROTEIN;

ARABIDOPSIS; ARTICLE; EPIMEDIUM; EPIMEDIUM SAGITTATUM; GENE STRUCTURE; GENETIC CONSERVATION; GENETIC VARIABILITY; INTRON; MOLECULAR CLONING; MYB GENE; PHYLOGENY; PLANT GENE; PROTEIN DETERMINATION; PROTEIN ISOLATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS; AMINO ACID SEQUENCE; CHEMISTRY; DNA SEQUENCE; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

ARABIDOPSIS; EPIMEDIUM; EPIMEDIUM SAGITTATUM;

AMINO ACID SEQUENCE; EPIMEDIUM; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; MOLECULAR SEQUENCE DATA; PHYLOGENY; PLANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION FACTORS;

EID: 84872200224     PISSN: 16616596     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms14010594     Document Type: Article

References (51)

1. Riechmann, J.L.; Heard, J.; Martin, G.; Reuber, L. Arabidopsis transcription factors: Genome-wide comparative analysis among eukaryotes. Science 2000, 290, 2105-2110.
2. Dubos, C.; Stracke, R.; Grotewold, E.; Weisshaar, B.; Martin, C.; Lepiniec, L. MYB transcription factors in Arabidopsis. Trends Plant Sci. 2010, 15, 573-581.
3. Lipsick, J.S. One billion years of Myb. Oncogene 1996, 13, 223-235.
4. Ogata, K.; Hojo, H.; Aimoto, S.; Nakai, T.; Nakamura, H.; Sarai, A.; Ishii, S.; Nishimura, Y. Solution structure of a DNA-binding unit of Myb: A helix-turn-helix-related motif with conserved tryptophans forming a hydrophobic core. Proc. Natl. Aca. Sci. USA 1992, 89, 6428-6432.
5. Stracke, R.; Werber, M.; Weisshaar, B. The R2R3-MYB gene family in Arabidopsis thaliana. Curr. Opin. Plant Biol. 2001, 4, 447-456.
6. Jiang, C.; Gu, X.; Peterson, T. Identification of conserved gene structures and carboxy-terminal motifs in the Myb gene family of Arabidopsis and Oryza sativa L. ssp. indica. Genome Biol. 2004, 5, R46.
7. Wilkins, O.; Nahal, H.; Foong, J.; Provart, N.J.; Campbell, M.M. Expansion and diversification of the Populus R2R3-MYB family of transcription factors. Plant Physiol. 2009, 149, 981-993.
8. Matus, J.T.; Aquea, F.; Arce-Johnson, P. Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes. BMC Plant Biol. 2008, 8, 83.
9. Deluc, L.; Bogs, J.; Walker, A.R.; Ferrier, T.; Decendit, A.; Merillon, J.M.; Robinson, S.P.; Barrieu, F. The transcription factor VvMYB5b contributes to the regulation of anthocyanin and proanthocyanidin biosynthesis in developing grape berries. Plant Physiol. 2008, 147, 2041-2053.
10. Jin, H.; Martin, C. Multifunctionality and diversity within the plant MYB-gene family. Plant Mol. Biol. 1999, 41, 577-585.
11. Paz-Ares, J.; Ghosal, D.; Wienand, U.; Peterson, P.; Saedler, H. The regulatory c1 locus of Zea mays encodes a protein with homology to myb proto-oncogene products and with structural similarities to transcriptional activators. EMBO J. 1987, 6, 3553-3558.
12. Hichri, I.; Barrieu, F.; Bogs, J.; Kappel, C.; Delrot, S.; Lauvergeat, V. Recent advances in the transcriptional regulation of the flavonoid biosynthetic pathway. J. Exp. Botany 2011, 62, 2465-2483.
13. Nesi, N.; Jond, C.; Debeaujon, I.; Caboche, M.; Lepiniec, L. The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a key determinant for proanthocyanidin accumulation in developing seed. Plant Cell Online 2001, 13, 2099-2114.
14. Borevitz, J.O.; Xia, Y.; Blount, J.; Dixon, R.A.; Lamb, C. Activation tagging identifies a conserved MYB regulator of phenylpropanoid biosynthesis. Plant Cell Online 2000, 12, 2383-2393.
15. Bogs, J.; Jaffé, F.W.; Takos, A.M.; Walker, A.R.; Robinson, S.P. The grapevine transcription factor VvMYBPA1 regulates proanthocyanidin synthesis during fruit development. Plant Physiol. 2007, 143, 1347-1361.
16. Czemmel, S.; Stracke, R.; Weisshaar, B.; Cordon, N.; Harris, N.N.; Walker, A.R.; Robinson, S.P.; Bogs, J. The grapevine R2R3-MYB transcription factor VvMYBF1 regulates flavonol synthesis in developing grape berries. Plant Physiol. 2009, 151, 1513-1530.
17. Jeong, S.T.; Goto-Yamamoto, N.; Hashizume, K.; Kobayashi, S.; Esaka, M. Expression of VvmybA1 gene and anthocyanin accumulation in various grape organs. Am. J. Enol. Viticulture 2006, 57, 507-510.
18. Gonzalez, A.; Zhao, M.; Leavitt, J.M.; Lloyd, A.M. Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings. Plant J. 2008, 53, 814-827.
19. Broun, P. Transcriptional control of flavonoid biosynthesis: A complex network of conserved regulators involved in multiple aspects of differentiation in Arabidopsis. Curr. Opin. Plant Biol. 2005, 8, 272-279.
20. Ramsay, N.A.; Glover, B.J. MYB-bHLH-WD40 protein complex and the evolution of cellular diversity. Trends Plant Sci. 2005, 10, 63-70.
21. Guo, B.; Xiao, P. Comment on main species of herba epimedii. China J. Chin. Mater. Medica 2003, 28, 303-307.
22. Ma, H.; He, X.; Yang, Y.; Li, M.; Hao, D.; Jia, Z. The genus Epimedium: An ethnopharmacological and phytochemical review. J. Ethnopharmacol. 2011, 134, 519-541.
23. Wu, H.; Lien, E.J.; Lien, L.L. Chemical and Pharmacological Investigations of Epimedium Species: A Survey. In Progress in Drug Research; Birkhäuser: Berlin, Germany, 2003; pp. 1-57.
24. Commission, P. Pharmacopoeia of the People's Republic of China. Chemical Industry Press: Beijing, China, 2005.
25. Zeng, S.; Xiao, G.; Guo, J.; Fei, Z.; Xu, Y.; Roe, B.A.; Wang, Y. Development of a EST dataset and characterization of EST-SSRs in a traditional Chinese medicinal plant, Epimedium sagittatum (Sieb. Et Zucc.) Maxim. BMC Genomics 2010, 11, 94.
26. Shen, P.; Guo, B.; Gong, Y.; Hong, D.Y.Q.; Hong, Y.; Yong, E. Taxonomic, genetic, chemical and estrogenic characteristics of Epimedium species. Phytochemistry 2007, 68, 1448-1458.
27. Xie, P.S.; Yan, Y.Z.; Guo, B.L.; Lam, C.; Chui, S.; Yu, Q.X. Chemical pattern-aided classification to simplify the intricacy of morphological taxonomy of Epimedium species using chromatographic fingerprinting. J. Pharmaceutical Biomed. Anal. 2010, 52, 452-460.
28. Li, Z.; Xu, Y.; Wang, Y.; Huang, H. Status and prospect of research on medicinal plants of Epimedium L. Chin. Tradit. Herbal Drugs 2005, 36, 289-295.
29. Kranz, H.D.; Denekamp, M.; Greco, R.; Jin, H.; Leyva, A.; Meissner, R.C.; Petroni, K.; Urzainqui, A.; Bevan, M.; Martin, C. Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana. Plant J. 1998, 16, 263-276.
30. Jin, H.; Cominelli, E.; Bailey, P.; Parr, A.; Mehrtens, F.; Jones, J.; Tonelli, C.; Weisshaar, B.; Martin, C. Transcriptional repression by AtMYB4 controls production of UV-protecting sunscreens in Arabidopsis. EMBO J. 2000, 19, 6150-6161.
31. Terrier, N.; Torregrosa, L.; Ageorges, A.; Vialet, S.; Verries, C.; Cheynier, V.; Romieu, C. Ectopic expression of VvMybPA2 promotes proanthocyanidin biosynthesis in grapevine and suggests additional targets in the pathway. Plant Physiol. 2009, 149, 1028-1041.
32. Galego, L.; Almeida, J. Role of DIVARICATA in the control of dorsoventral asymmetry in Antirrhinum flowers. Genes Dev. 2002, 16, 880-891.
33. Howarth, D.G.; Donoghue, M.J. Duplications and expression of DIVARICATA-like genes in Dipsacales. Mol. Biol. Evolut. 2009, 26, 1245-1258.
34. Zimmermann, I.M.; Heim, M.A.; Weisshaar, B.; Uhrig, J.F. Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B like BHLH proteins. Plant J. 2004, 40, 22-34.
35. Deluc, L.; Barrieu, F.; Marchive, C.; Lauvergeat, V.; Decendit, A.; Richard, T.; Carde, J.P.; Mérillon, J.M.; Hamdi, S. Characterization of a grapevine R2R3-MYB transcription factor that regulates the phenylpropanoid pathway. Plant Physiol. 2006, 140, 499-511.
36. Zhou, X.R.; Wang, Y.Z.; Smith, J.F.; Chen, R. Altered expression patterns of TCP and MYB genes relating to the floral developmental transition from initial zygomorphy to actinomorphy in Bournea (Gesneriaceae). New Phytol. 2008, 178, 532-543.
37. Tamagnone, L.; Merida, A.; Parr, A.; Mackay, S.; Culianez-Macia, F.A.; Roberts, K.; Martin, C. The AmMYB308 and AmMYB330 transcription factors from Antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco. Plant Cell Online 1998, 10, 135-154.
38. Aharoni, A.; de Vos, C.; Wein, M.; Sun, Z.; Greco, R.; Kroon, A.; Mol, J.N.M.; O'Connell, A.P. The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J. 2001, 28, 319-332.
39. Yoshida, K.; Iwasaka, R.; Kaneko, T.; Sato, S.; Tabata, S.; Sakuta, M. Functional differentiation of Lotus japonicus TT2s, R2R3-MYB transcription factors comprising a multigene family. Plant Cell Physiol. 2008, 49, 157-169.
40. Tamura, K.; Dudley, J.; Nei, M.; Kumar, S. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evolution 2007, 24, 1596-1599.
41. Pérez-Rodríguez, P.; Riaño-Pachón, D.M.; Corrêa, L.G.G.; Rensing, S.A.; Kersten, B.; Mueller-Roeber, B. PlnTFDB: Updated content and new features of the plant transcription factor database. Nucleic Acids Res. 2010, 38, D822-D827.
42. Grotewold, E.; Sainz, M.B.; Tagliani, L.; Hernandez, J.M.; Bowen, B.; Chandler, V.L. Identification of the residues in the Myb domain of maize C1 that specify the interaction with the bHLH cofactor R. Proc. Natl. Aca. Sci. USA 2000, 97, 13579-13584.
43. Yanhui, C.; Xiaoyuan, Y.; Kun, H.; Meihua, L.; Jigang, L.; Zhaofeng, G.; Zhiqiang, L.; Yunfei, Z.; Xiaoxiao, W.; Xiaoming, Q. The MYB transcription factor superfamily of Arabidopsis: Expression analysis and phylogenetic comparison with the rice MYB family. Plant Mol. Biol. 2006, 60, 107-124.
44. Legay, S.; Lacombe, E.; Goicoechea, M.; Brière, C.; Séguin, A.; Mackay, J.; Grima-Pettenati, J. Molecular characterization of EgMYB1, a putative transcriptional repressor of the lignin biosynthetic pathway. Plant Sci. 2007, 173, 542-549.
45. Doyle, J.; Doyle, J. Isolation of plant DNA from fresh tissue. Focus 1990, 12, 13-15.
46. Altschul, S.F.; Gish, W.; Miller, W.; Myers, E.W.; Lipman, D.J. Basic local alignment search tool. J. Mol. Biol. 1990, 215, 403-410.
47. Wheeler, D.L.; Church, D.M.; Federhen, S.; Lash, A.E.; Madden, T.L.; Pontius, J.U.; Schuler, G.D.; Schriml, L.M.; Sequeira, E.; Tatusova, T.A.; et al. Database resources of the National Center for Biotechnology. Nucleic Acids Res. 2003, 31, 28-33.
48. Wheelan, S.J.; Church, D.M.; Ostell, J.M. Spidey: A tool for mRNA-to-genomic alignments. Genome Res. 2001, 11, 1952-1957.
49. Larkin, M.A.; Blackshields, G.; Brown, N.P.; Chenna, R.; McGettigan, P.A.; McWilliam, H.; Valentin, F.; Wallace, I.M.; Wilm, A.; Lopez, R.; Thompson, J.D.; Gibson, T.J.; Higgins, D.G. Clustal W and Clustal X version 2.0. Bioinformatics 2007, 23, 2947-2948.
50. -ΔΔCT method. Methods 2001, 25, 402-408.
51. Mancinelli, A. Interaction between light quality and light quantity in the photoregulation of anthocyanin production. Plant Physiol. 1990, 92, 1191-1195.