Association of soybean-based extenders with field fertility of stored ram (Ovis aries) semen: A randomized double-blind parallel group design

Theriogenology

Volumn 79, Issue 3, 2013, Pages 517-527

  Khalifa, Tarek ^a   Lymberopoulos, Aristotelis ^b   Theodosiadou, Ekaterini ^c  

^a EquiBiotech Inc Research Services in Farm Animal Breeding   (Greece)

^b ATEI of Thessaloniki ^*  (Greece)

^c UNIVERSITY OF THESSALY   (Greece)

Author keywords

AndroMed; Artificial insemination; BioXcell; Pregnancy rate; Sheep; Spermatozoa

Indexed keywords

CRYOPROTECTIVE AGENT; PHOSPHATIDYLCHOLINE;

ANIMAL; ANIMAL DISEASE; ARTICLE; ARTIFICIAL INSEMINATION; COLD; COMPARATIVE STUDY; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; CRYOPRESERVATION; DOUBLE BLIND PROCEDURE; ESTRUS CYCLE; FEMALE; FERTILITY; MALE; METHODOLOGY; PHYSIOLOGY; PREGNANCY; PREGNANCY RATE; RANDOMIZED CONTROLLED TRIAL; SHEEP; SOYBEAN; SPERM PRESERVATION; SPERMATOZOON;

ANIMALS; COLD TEMPERATURE; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; DOUBLE-BLIND METHOD; ESTRUS SYNCHRONIZATION; FEMALE; FERTILITY; INSEMINATION, ARTIFICIAL; LECITHINS; MALE; PREGNANCY; PREGNANCY RATE; SEMEN PRESERVATION; SHEEP, DOMESTIC; SOYBEANS; SPERMATOZOA;

GLYCINE MAX; OVIS ARIES;

EID: 84872182572     PISSN: 0093691X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.theriogenology.2012.11.009     Document Type: Article

References (82)

1. Anel L., Alvarez M., Martinez-Pastor F., Garcia-Macias V., Anel E., de Paz P. Improvement strategies in ovine artificial insemination. Reprod Dom Anim 2006, 41(Suppl. 2):30-42.
2. Salamon S., Maxwell W.M.C., Firth J.H. Fertility of semen after storage at 5 °C. Anim Reprod Sci 1979, 2:373-385.
3. Chemineau P., Cagnie Y., Guerin Y., Orgeur P., Vallet J.C. Training manual on artificial insemination in sheep and goats 1991, Food and Agriculture Organization of the United Nations, Rome.
4. Paulenz H., Soderquist L., Perez-Pe R., Andersen Berg K. Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology 2002, 57:823-836.
5. Fernandez-Abella D., Preve M.O., Villegas N. Insemination time and dilution rate of cooled ram semen affects fertility. Theriogenology 2003, 60:21-26.
6. Ehling C., Wirth P., Schindler L., Hadeler K.G., Dopke H.H., Lemme E., et al. Laparoscopical intratuterine insemination with different doses of fresh, conserved, and frozen semen for the production of ovine zygotes. Theriogenology 2003, 60:777-787.
7. Halbert G.W., Dobson H., Walton J.S., Buckrell B.C. The structure of the cervical canal of the ewe. Theriogenology 1990, 33:977-992.
8. Kaabi M., Alvarez M., Anel E., Chamorro C.A., Boixo J.C., de Paz P., et al. Influence of breed and age on morphometry and depth of inseminating catheter penetration in the ewe cervix: a postmortem study. Theriogenology 2006, 66:1876-1883.
9. Sanchez-Partida L.G., Windsor D.P., Eppleston J., Setchell B.P., Maxwell W.M.C. Fertility and its relationship to motility characteristics of spermatozoa in ewes after cervical, transcervical, and intrauterine insemination with frozen-thawed ram semen. J Androl 1999, 20:280-288.
10. Byrne G.P., Lonergan P., Wade M., Duffy P., Donovan A., Hanrahan J.P., et al. Effect of freezing rate of ram spermatozoa on subsequent fertility in vivo and in vitro. Anim Reprod Sci 2000, 62:265-275.
11. ® and used for cervical artificial insemination. Theriogenology 2003, 59:1157-1170.
12. Paulenz H., Soderquist L., Adnoy T., Harald Fossen O., Andersen Berg K. Effect of milk- and Tris-based extenders on the fertility of sheep inseminated vaginally once or twice with liquid semen. Theriogenology 2003, 60:759-766.
13. O'Meara C.M., Hanrahan J.P., Prathalingam N.S., Owen J.S., Donovan A., Fair S., et al. Relationship between in vitro sperm functional tests and in vivo fertility of rams following cervical artificial insemination of ewes with frozen-thawed semen. Theriogenology 2008, 69:513-522.
14. Papadopoulos S., Hanrahan J.P., Donovan A., Duffy P., Boland M.P., Lonergan P. In vitro fertilization as a predictor of fertility from cervical insemination of sheep. Theriogenology 2005, 63:150-159.
15. O'Meara C.M.O., Hanrahan J.P., Donovan A., Fair S., Rizos D., Wade M., et al. Relationship between in vitro fertilization of ewes oocytes and the fertility of ewes following cervical artificial insemination with frozen-thawed ram semen. Theriogenology 2005, 64:1797-1808.
16. Windsor D.P. Variation between ejaculates in the fertility of frozen ram semen used for cervical insemination of Merino ewes. Anim Reprod Sci 1997, 47:21-29.
17. Donovan A., Hanrahan J.P., Kummen E., Duffy P., Boland M.P. Fertility in the ewe following cervical insemination with fresh or frozen-thawed semen at a natural and synchronised oestrus. Anim Reprod Sci 2004, 84:359-368.
18. Anel L., Kabbi M., Abroug B., Alvarez M., Anel E., Boixo J.C., et al. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay. Theriogenology 2005, 63:1235-1247.
19. Fair S., Hanrahan J.P., O'Meara, Duffy P., Rizos D., Wade M., et al. Differences between Belclare and Suffolk ewes in fertilization rate, embryo quality and accessory sperm number after cervical or laparoscopic artificial insemination. Theriogenology 2005, 63:1995-2005.
20. Khalifa T.A.A., Lymberopoulos A.G., Belibasaki S., Vainas E., Saratsi E. Impact of lactation on the fecundity of ewes (Abstract). Reprod Dom Anim 2006, 41:309.
21. Fukui Y., Kohno H., Okabe K., Katsuki S., Yoshizawa M., Togari T., et al. Factors affecting the fertility of ewes after intrauterine insemination with frozen-thawed semen during the non-breeding season. J Reprod Dev 2010, 56:460-466.
22. O'Hara L., Hanrahan J.P., Richardson L., Donovan A., Fair S., Evans A.C.O., et al. Effect of storage duration, storage temperature, and diluents on the viability and fertility of fresh ram semen. Theriogenology 2010, 73:541-549.
23. Palacin I., Yaniz J.L., Fantova E., Blasco M.E., Quintin-Casorran F.J., Sevilla-Mur E., et al. Factors affecting fertility after cervical insemination with cooled semen in meat sheep. Anim Reprod Sci 2012, 132:139-144.
24. Graham E.F., Crabo B.G., Pace M.M. Current status of semen preservation in the ram, boar and stallion. J Anim Sci 1978, 47(Suppl. 2):80-119.
25. Maxwell W.M., Salamon S. Liquid storage of ram semen. Reprod Fertil Dev 1993, 5:613-638.
26. Salamon S., Maxwell W.M.C. Frozen storage of ram semen. I. Processing, freezing, thawing and fertility after cervical insemination. Anim Reprod Sci 1995, 37:185-249.
27. Salamon S., Maxwell W.M. Storage of ram semen. Anim Reprod Sci 2000, 62:77-111.
28. Watson P.F. The roles of lipid and protein in the protection of ram spermatozoa at 5 °C by egg-yolk lipoprotein. J Reprod Fert 1981, 62:483-492.
29. Watson P.F. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod Fertil Dev 1995, 7:871-891.
30. Bergeron A., Manjunath P. New insights towards understanding the mechanisms of sperm protection by egg yolk and milk. Mol Reprod Dev 2006, 73:1338-1344.
31. Moustacas V.S., Zaffalon F.G., Lagares M.A., Loaiza-Eccheverri A.M., Varago F.C., Neves M.M., et al. Natural, but not lyophilized, low density proteins were an acceptable alternative to egg yolk for cryopreservation of ram semen. Theriogenology 2011, 75:300-307.
32. Watson P.F., Martin I.C.A. Artificial insemination of sheep: the effect of semen diluents containing egg yolk on the fertility of ram semen. Theriogenology 1976, 6:559-564.
33. Maxwell W.M.C., Evans G., Mortimer S.T., Gillan L., Gellatly E.S., McPhie C.A. Normal fertility in ewes after cervical insemination with frozen-thawed spermatozoa supplemented with seminal plasma. Reprod Fertil Dev 1999, 11:123-126.
34. Belibasaki S., Amiridis G.S., Lymberopoulos A., Varsakeli S., Kouskoura T. Ram seminal plasma and fertility: results from an ongoing field study. Acta Vet Hung 2000, 48:335-341.
35. Lopez-Perez A., Perez-Clariget R. Ram seminal plasma improves pregnancy rates in ewes cervically inseminated with ram semen stored at 5 °C for 24 hours. Theriogenology 2012, 77:395-399.
36. Matsuoka T., Imai H., Kohno H., Fukui Y. Effects of bovine serum albumin and trehalose in semen diluents for improvement of frozen-thawed ram spermatozoa. J Reprod Dev 2006, 52:675-683.
37. Fukui Y., Kohno H., Togari T., Hiwasa M. Fertility of ewes inseminated intrauterinally with frozen semen using extender containing bovine serum albumin. J Reprod Dev 2007, 53:959-962.
38. Fukui Y., Kohno H., Togari T., Hiwasa M., Okabe K. Fertility after artificial insemination using a soybean-based semen extender in sheep. J Reprod Dev 2008, 54:286-289.
39. Muro J., Alvarez M., Garcia-Macias V., Martinez-Pastor F., Gonzalez N., Bernardo J., et al. Effect of five extenders on the fertility of refrigerated ram semen (Abstract). Reprod Dom Anim 2006, 41:327.
40. Bielanski A. Disinfection procedures for controlling microorganisms in the semen and embryos of humans and farm animals. Theriogenology 2007, 68:1-22.
41. Wrathall A.E., Holyoak G.R., Parsonson I.M., Simmons H.A. Risks of transmitting ruminant spongiform encephalopathies (prion diseases) by semen and embryo transfer techniques. Theriogenology 2008, 70:725-745.
42. de Paz P., Esteso M.C., Alvarez M., Mata M., Chamorro C.A., Anel L. Development of extender based on soybean lecithin for its application in liquid ram semen. Theriogenology 2010, 74:663-671.
43. Forouzanfar M., Sharafi M., Hosseini S.M., Ostadhosseini S., Hajian M., Hosseini L., et al. In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology 2010, 73:480-487.
44. Gil J., Lundeheim N., Soderquist L., Rodriguez-Martinez H. Influence of extender, temperature, and addition of glycerol on post-thaw sperm parameters in ram semen. Theriogenology 2003, 59:1241-1255.
45. Hiwasa M., Kohno H., Togari T., Okabe K., Fukui Y. Fertility after different artificial insemination methods using a synthetic semen extender in sheep. J Reprod Dev 2009, 55:50-54.
46. Kulaksiz R., Cebl C., Akcay E. The effect of different extenders on the motility and morphology of ram sperm frozen or stored at 4 °C. Turk J Vet Anim Sci 2012, 36:177-182.
47. Avdi M., Banos G., Kouttos A., Bodin L., Chemineau P. Sources of variation and genetic profile of spontaneous, out-of-season ovulatory activity in the Chios sheep. Genet Sel Evol 2003, 35:65-76.
48. Alexopoulos K., Karagiannidis A., Tsakalof P. Development of macroscopic and microscopic characteristics of ejaculates from Chios, Serres and Karaguniki breed lambs. Theriogenology 1991, 36:667-680.
49. Belibasaki S., Kouimtzis S. Sexual activity and body and testis growth in prepubertal ram lambs of Friesland, Chios, Karagouniki and Serres dairy sheep in Greece. Small Rumin Res 2000, 37:109-113.
50. Simitzis P.E., Deligeorgis S.G., Bizelis J.A. Effect of breed and age on sexual behaviour of rams. Theriogenology 2006, 65:1480-1491.
51. Lymberopoulos A., Tsakmakidis I.A., Khalifa T.A.A. Effect of ram age on structural and functional competence of frozen-thawed spermatozoa in dairy sheep. Reprod Dom Anim 2010, 45:572-578.
52. Marti J.I., Aparicio I.M., Garcia-Herreros M. Head morphometric changes in cryopreserved ram spermatozoa are related to sexual maturity. Theriogenology 2011, 75:473-481.
53. Marti J.I., Aparicio I.M., Garcia-Herreros M. Sperm morphometric subpopulations are differentially distributed in rams with different maturity age in cryopreserved ejaculates. Theriogenology 2011, 76:97-109.
54. Russel A. Body condition scoring of sheep. Sheep and goat practice 1991, 3. Bailliere Tindall, Philadelphia. E. Boden (Ed.).
55. Upreti G.C., Oliver J.E., Duganzich D.M., Munday R., Smith J.F. Development of a chemically defined semen diluent (RSD-1). Anim Reprod Sci 1995, 37:143-157.
56. Kuhholzer B., Besenfelder U., Muller S., Reichenbach H.D., Brem G. Laparoscopic insemination of seasonally anoestrous ewes by a simplified method under field conditions. Reprod Dom Anim 1997, 32:309-312.
57. Lymberopoulos A., Kuhholzer B., Amiridis G.S., Christodoulou V., Vainas E. Laparoscopic intrauterine insemination in superovulated Chios breed ewes. Bull Hell Vet Med Soc 1999, 50:321-325.
58. Lymberopoulos A., Amiridis G.S., Kuhholzer B., Besenfelder U., Christodoulou V., Vainas E., et al. Fertilization and embryo recovery rates in superovulated Chios ewes after laparoscopic intrauterine insemination. Theriogenology 2001, 55:1855-1862.
59. Mieusset R., Quintana Casares P., Sanchez Partida L.G., Sowerbutts S.F., Zupp J.L., Setchell B.P. Effects of heating the testes and epididymides of rams by scrotal insulation on fertility and embryonic mortality in ewes inseminated with frozen semen. J Reprod Fert 1992, 94:337-343.
60. Petrie A., Sabin C. Medical statistics at a glance 2005, Blackwell Publishing, Oxford. Second Edition.
61. Khalifa TAA, Lymberopoulos AG. Changeability of sperm chromatin structure during liquid storage of ovine semen in milk-egg yolk- and soybean lecithin-based extenders and their relationships to field-fertility. Cell Tissue Bank, in press.
62. Khalifa T.A.A., Lymberopoulos A.G. Chromatin integrity and fertility of frozen ram spermatozoa (Abstract). Reprod Dom Anim 2011, 46(Suppl. 3):118.
63. Petrie A., Watson P. Statistics for veterinary and animal science 2006, Blackwell Publishing, Oxford. Second Edition.
64. Murray R.D., Ward W.R. Welfare implications of modern artificial breeding techniques for dairy cattle and sheep. Vet Rec 1993, 133:283-286.
65. Avdi M., Driancourt M.A., Chemineau P. Seasonal variation in oestrous behaviour and ovulatory activity in Chios and Serres ewes in Greece. Reprod Nutr Dev 1993, 33:15-24.
66. Avdi M., Banos G., Stefos K., Chemineau P. Seasonal variation in testicular volume and sexual behavior of Chios and Serres rams. Theriogenology 2004, 62:275-282.
67. Gillan L., Maxwell W.M.C., Gareth E. Preservation and evaluation of semen for artificial insemination. Reprod Fertil Dev 2004, 16:447-454.
68. Salamon S., Maxwell W.M.C. Frozen storage of ram semen. II. Causes of low fertility after cervical insemination and methods of improvement. Anim Reprod Sci 1995, 38:1-36.
69. Druart X., Cognie J., Baril G., Clement F., Dacheux J.-L., Gatti J- L. In vivo imaging of in situ motility of fresh and liquid stored spermatozoa in the ewe genital tract. Reproduction 2009, 138:45-53.
70. Mann T., Lutwak-Mann C. Male reproductive function and semen 1981, Springer-Verlag, New York, Berlin, Heidelberg. Second Edition.
71. Lloyd R.E., Fazeli A., Watson P.F., Holt W.V. The oviducal protein, heat-shock 70-kDa protein 8, improves the long-term survival of ram spermatozoa during storage at 17 °C in a commercial extender. Reprod Fertil Dev 2012, 24:543-549.
72. Khalifa T.A.A., Lymberopoulos A.G., Tsanikidis N., Belibasaki S. Quality and fertility of Chios ram semen diluted in extenders supplemented with or without ingredients of animal origin (Abstract) February 16-19, 2006, Tenth Panhellenic Veterinary Congress, Athens, Greece, p. 185.
73. Ruttle J.L., Southward G.M. Influence of age and scrotal circumference on breeding soundness examination of range rams. Theriogenology 1988, 29:945-949.
74. Kasimanickam R., Pelzer K.D., Kasimanickam V., Swecker W.S., Thatcher C.D. Association of classical semen parameters, sperm DNA fragmentation index, lipid peroxidation and antioxidant enzymatic activity of semen in ram-lambs. Theriogenology 2006, 65:1407-1421.
75. Stradaioli G., Noro T., Sylla L., Monaci M. Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders. Theriogenology 2007, 67:1249-1255.
76. Gundogan M., Yeni D., Avdatek F., Fidan A.F. Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage. Anim Reprod Sci 2010, 122:200-207.
77. Karagiannidis A., Varsakeli S., Alexopoulos C., Amarantidis I. Seasonal variation in semen characteristics of Chios and Friesian rams in Greece. Small Rumin Res 2000, 37:125-130.
78. Osinowo O.A., Ahmed M.S., Ekpe G.A. Semen quality and sperm output of Yankasa rams at different ages. Theriogenology 1988, 29:381-386.
79. Moses D., Martinez A.G., Iorio G., Valcarcel A., Ham A., Pessi H., et al. A large-scale program in laparoscopic intrauterine insemination with frozen-thawed semen in Australian Merino sheep in Argentine Patagonia. Theriogenology 1997, 48:651-657.
80. Manes J., Fiorentino M.A., Kaiser G., Hozbor F., Alberio R., Sanchez E., et al. Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes. Small Rumin Res 2010, 94:201-204.
81. Zarkawi M. Monitoring the reproductive performance in Awassi ewes using progesterone radioimmunoassay. Small Rumin Res 1997, 26:291-294.
82. Paulenz H., Adnoy T., Fossen O.H., Soderquist L. Effect on field fertility of addition of gelatin, different dilution rates and storage times of cooled ram semen after vaginal insemination. Reprod Dom Anim 2010, 45:706-710.