A propionate-inducible expression system based on the Corynebacterium glutamicum prpD2 promoter and PrpR activator and its application for the redirection of amino acid biosynthesis pathways

Journal of Biotechnology

Volumn 163, Issue 2, 2013, Pages 225-232

  Plassmeier, Jens K ^a,b   Busche, Tobias ^a   Molck, Stella ^a   Persicke, Marcus ^a   Pühler, Alfred ^a   Rückert, Christian ^a   Kalinowski, Jörn ^a  

^a BIELEFELD UNIVERSITY   (Germany)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Corynebacterium glutamicum; Expression system; Propionate; PrpR

Indexed keywords

BIOSYNTHESIS PATHWAYS; BRANCHING POINTS; CHLORAMPHENICOL ACETYL TRANSFERASE; COMPLEX MEDIA; CORYNEBACTERIUM GLUTAMICUM; EXPRESSION SYSTEM; GROWTH MEDIUM; HOMOSERINE; INDUCED TRANSCRIPTION; INDUSTRIAL FERMENTATION; NATURAL SYSTEMS; PROOF OF PRINCIPLES; PROPIONATE; PRPR; REPORTER SYSTEMS; TRANSCRIPTIONAL ACTIVATORS;

AMINO ACIDS; BIOCHEMISTRY; GENE ENCODING; METABOLISM; PHYSIOLOGY; TRANSCRIPTION;

VOLATILE FATTY ACIDS;

AMINO ACID; CHLORAMPHENICOL ACETYLTRANSFERASE; HOMOSERINE; HOMOSERINE DEHYDROGENASE; ISOLEUCINE; LYSINE; PROPIONIC ACID; THREONINE;

AMINO ACID SYNTHESIS; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM CULTURE; BIOSYNTHESIS; CAT GENE; CATABOLISM; CONCENTRATION (PARAMETERS); CORYNEBACTERIUM GLUTAMICUM; CULTURE MEDIUM; FATTY ACID METABOLISM; FERMENTATION; GENE; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; GENETIC TRANSCRIPTION; LARGE SCALE PRODUCTION; NONHUMAN; OPERON; PRIORITY JOURNAL; PRPD2 GENE; PRPR GENE; REPORTER GENE; RNA TRANSLATION; SUPERNATANT; TRANSCRIPTION REGULATION;

AMINO ACIDS; BACTERIAL PROTEINS; CARBON CYCLE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CITRATES; CORYNEBACTERIUM GLUTAMICUM; FERMENTATION; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENES, BACTERIAL; METABOLIC ENGINEERING; METABOLIC NETWORKS AND PATHWAYS; OPERON; PROMOTER REGIONS, GENETIC; PROPIONATES; TRANSCRIPTION FACTORS;

CORYNEBACTERIUM GLUTAMICUM;

EID: 84872180180     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.08.009     Document Type: Article

References (37)

1. Amann E., Brosius J., Ptashne M. Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 1983, 25:167-178.
2. Blombach B., Riester T., Wieschalka S., Ziert C., Youn J.W., Wendisch V.F., Eikmanns B.J. Corynebacterium glutamicum tailored for efficient isobutanol production. Applied and Environment Microbiology 2011, 77:3300-3310.
3. Cagnon C., Valverde V., Masson J.M. A new family of sugar-inducible expression vectors for Escherichia coli. Protein Engineering 1991, 4:843-847.
4. Claes W.A., Pühler A., Kalinowski J. Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle. Journal of Bacteriology 2002, 184:2728-2739.
5. de Boer H.A., Comstock L.J., Vasser M. The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academic Science of the United States of America 1983, 80:21-25.
6. Figge J., Wright C., Collins C.J., Roberts T.M., Livingston D.M. Stringent regulation of stably integrated chloramphenicol acetyl transferase genes by E. coli lac repressor in monkey cells. Cell 1988, 52:713-722.
7. Horton R.M., Hunt H.D., Ho S.N., Pullen J.K., Pease L.R. Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension. Gene 1989, 77:61-68.
8. Hüser A.T., Becker A., Brune I., Dondrup M., Kalinowski J., Plassmeier J., Pühler A., Wiegräbe I., Tauch A. Development of a Corynebacterium glutamicum DNA microarray and validation by genome-wide expression profiling during growth with propionate as carbon source. Journal of Biotechnology 2003, 106:269-286.
9. Ikeda M., Nakagawa S. The Corynebacterium glutamicum genome: features and impacts on biotechnological processes. Applied Microbiology and Biotechnology 2003, 62:99-109.
10. Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Krämer R., Linke B., McHardy A.C., Meyer F., Möckel B., Pfefferle W., Pühler A., Rey D.A., Rückert C., Rupp O., Sahm H., Wendisch V.F., Wiegräbe I., Tauch A. The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins. Journal of Biotechnology 2003, 104:5-25.
11. Kawaguchi H., Sasaki M., Vertes A.A., Inui M., Yukawa H. Identification and functional analysis of the gene cluster for l-arabinose utilization in Corynebacterium glutamicum. Applied and Environment Microbiology 2009, 75:3419-3429.
12. Kelle R., Herrmann T., Bathe B. l-Lysine production. Handbook of Corynebacterium glutamicum 2005, Taylor & Francis Group, Boca Raton, FL, pp. 465-485.
13. Kimura E. l-Glutamate production. Handbook of Corynebacterium glutamicum 2005, Taylor & Francis Group, Boca Raton, FL, pp. 439-464.
14. Lee S.K., Keasling J.D. A propionate-inducible expression system for enteric bacteria. Applied and Environment Microbiology 2005, 71:6856-6862.
15. Lee S.K., Keasling J.D. A Salmonella-based, propionate-inducible, expression system for Salmonella enterica. Gene 2006, 377:6-11.
16. Lee S.K., Keasling J.D. Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methods. Protein Expression and Purification 2008, 61:197-203.
17. Litsanov B., Brocker M., Bott M. Toward homosuccinate fermentation: metabolic engineering of Corynebacterium glutamicum for anaerobic production of succinate from glucose and formate. Applied and Environment Microbiology 2012, 78:3325-3337.
18. Niimi S., Suzuki N., Inui M., Yukawa H. Metabolic engineering of 1,2-propanediol pathways in Corynebacterium glutamicum. Applied Microbiology and Biotechnology 2011, 90:1721-1729.
19. Ohnishi J., Mitsuhashi S., Hayashi M., Ando S., Yokoi H., Ochiai K., Ikeda M. A novel methodology employing Corynebacterium glutamicum genome information to generate a new l-lysine-producing mutant. Applied Microbiology and Biotechnology 2002, 58:217-223.
20. Okibe N., Suzuki N., Inui M., Yukawa H. Isolation, evaluation and use of two strong, carbon source-inducible promoters from Corynebacterium glutamicum. Letters in Applied Microbiology 2010, 50:173-180.
21. Park J.U., Jo J.H., Kim Y.J., Chung S.S., Lee J.H., Lee H.H. Construction of heat-inducible expression vector of Corynebacterium glutamicum and C. ammoniagenes: fusion of lambda operator with promoters isolated from C. ammoniagenes. Journal of Microbiology and Biotechnology 2008, 18:639-647.
22. Persicke M., Rückert C., Plassmeier J., Stutz L., Kessler N., Kalinowski J., Goesmann A., Neuweger H. MSEA: metabolite set enrichment analysis in the MeltDB metabolomics software platform: metabolic profiling of Corynebacterium glutamicum as an example. Metabolomics 2012, 8:310-322.
23. Plassmeier J., Barsch A., Persicke M., Niehaus K., Kalinowski J. Investigation of central carbon metabolism and the 2-methylcitrate cycle in Corynebacterium glutamicum by metabolic profiling using gas chromatography-mass spectrometry. Journal of Biotechnology 2007, 130:354-363.
24. Plassmeier J., Persicke M., Pühler A., Sterthoff C., Rückert C., Kalinowski J. Molecular characterization of PrpR, the transcriptional activator of propionate catabolism in Corynebacterium glutamicum. Journal of Biotechnology 2012, 159:1-11.
25. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual 1989, Cold Spring Harbor Laboratory Press, New York. second ed.
26. Schäfer A., Tauch A., Jäger W., Kalinowski J., Thierbach G., Pühler A. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 1994, 145:69-73.
27. Schleif R. Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics 2000, 16:559-565.
28. Song Y., Matsumoto K., Yamada M., Gohda A., Brigham C.J., Sinskey A.J., Taguchi S. Engineered Corynebacterium glutamicum as an endotoxin-free platform strain for lactate-based polyester production. Applied Microbiology and Biotechnology 2012, 93:1917-1925.
29. Su T.Z., Schweizer H., Oxender D.L. A novel phosphate-regulated expression vector in Escherichia coli. Gene 1990, 90:129-133.
30. Tauch A., Götker S., Pühler A., Kalinowski J., Thierbach G. The 27.8-kb R-plasmid pTET3 from Corynebacterium glutamicum encodes the aminoglycoside adenyltransferase gene cassette aadA9 and the regulated tetracycline efflux system Tet 33 flanked by active copies of the widespread insertion sequence IS6100. Plasmid 2002, 48:117-129.
31. Tauch A., Kassing F., Kalinowski J., Pühler A. The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid 1995, 34:119-131.
32. Tauch A., Kirchner O., Wehmeier L., Kalinowski J., Pühler A. Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli. FEMS Microbiology Letters 1994, 123:343-347.
33. Trötschel C., Deutenberg D., Bathe B., Burkovski A., Krämer R. Characterization of methionine export in Corynebacterium glutamicum. Journal of Bacteriology 2005, 187:3786-3794.
34. Trötschel C., Follmann M., Nettekoven J.A., Mohrbach T., Forrest L.R., Burkovski A., Marin K., Krämer R. Methionine uptake in Corynebacterium glutamicum by MetQNI and by MetPS, a novel methionine and alanine importer of the NSS neurotransmitter transporter family. Biochemistry 2008, 47:12698-12709.
35. Vašicová P., Abrhámová Z., Nešvera J., Pátek M., Sahm H.B.E. Integrative and autonomously replicating vectors for analysis of promoters in Corynebacterium glutamicum. Biotechnology Techniques 1998, 12:743-746.
36. Wieschalka S., Blombach B., Eikmanns B.J. Engineering Corynebacterium glutamicum for the production of pyruvate. Applied Microbiology and Biotechnology 2012, 94:449-459.
37. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 1985, 33:103-119.