Purification and characterization of Thermobifida fusca xynalase 10B

Canadian Journal of Microbiology

Volumn 50, Issue 10, 2004, Pages 835-843

  Kim, Jeong H ^a   Irwin, Diana ^b   Wilson, David B ^b  

^a Gyeongsang National University   (South Korea)

^b Department of Obstetrics Gynecology   (United States)

Author keywords

Enzyme purification; Family 10 hydrolase; Thermobifida fusca; Xylanase

Indexed keywords

CELLULOSE; CHARACTERIZATION; ESCHERICHIA COLI; GENES; HYDROLYSIS; MASS SPECTROMETRY;

GENE ENCODING; GLYCOHYDROLASE; THERMOSTABILITY;

PROTEINS;

4 NITROPHENYL ALPHA DEXTRO ARABINOFURANOSIDE; 4 NITROPHENYL BETA DEXTRO CELLOBIOSIDE; 4 NITROPHENYL BETA DEXTRO XYLOBIOSIDE; 4 NITROPHENYL BETA DEXTRO XYLOSIDE; CARBOHYDRATE DERIVATIVE; CELLOBIOSE; CELLULASE; ENDO 1,4 BETA XYLANASE; UNCLASSIFIED DRUG; XYLAN; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOBIOSE;

MICROBIOLOGY;

ARTICLE; BACTERIAL GROWTH; BIRCH; CARBOXY TERMINAL SEQUENCE; CORN; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE OVEREXPRESSION; HYDROLYSIS; MASS SPECTROMETRY; NONHUMAN; OAT; PH; PRIORITY JOURNAL; THERMOBIFIDA FUSCA; THERMOMONOSPORA; THERMOSTABILITY;

ACTINOMYCETALES; CELLULOSE; CLONING, MOLECULAR; DISACCHARIDES; ENDO-1,4-BETA XYLANASES; ENZYME INDUCTION; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TEMPERATURE; XYLANS;

CELLULOSE; GENES; HYDROLYSIS; MASS SPECTROSCOPY; PROTEINS; REDUCING SUGARS; XYLANASE;

EID: 14344251903     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/w04-077     Document Type: Article

References (28)

1. Aurillia, V., Martin, J.C., MCrae, S.I., Scott, K.P., Rincon, M.T., and Flint, H.J. 2000. Three multidomain esterases from the cellulolytic rumen anaerobe Ruminococcus flavefaciens 17 that carry divergent dockerin sequences. Microbiology, 146: 1391-1397.
2. Bachmann, S.L., and McCarthy, A.J. 1991. Purification and cooperative activity of enzymes constituting the xylan-degrading system of Thermomonospora fusca. Appl. Environ. Microbiol. 57: 2121-2130.
3. Beg, Q.K., Kapoor, M., Mahajan, L., and Hoondal, G.S. 2001. Microbial xylanases and their industrial applications: a review. Appl. Microbiol Biotechnol. 56: 326-338.
4. Blanco, J., Coque, J.J., Velasco, J., and Martin, J.F. 1997. Cloning, expression in Streptomyces lividans and biochemical characterization of a thermostable endo-beta-1,4-xylanase of Thermomonospora alba ULJB1 with cellulose-binding ability. Appl. Microbiol. Biotechnol 48: 208-217.
5. Caufrier, F., Martinou, A., Dupont, C., and Bouriotis, V. 2003. Carbohydrate esterase family 4 enzymes: substrate specificity. Carbohydr. Res. 338: 687-692.
6. Clarke, J.H., Davidson, K., Gilbert, H.J., Fontes, C.M.G.A., and Hazlewood, G.P. 1996. A modular xylanase from mesophilic Cellulomonas fimi contains the same cellulose-binding and thermostabilizing domains as xylanases from thermophilic bacteria. FEMS Microbiol. Lett. 139: 27-35.
7. Fernandes, A.C., Fontes, C.M.G.A., Gilbert, H.J., Hazlewood, G.P., Fernandes, T.H., and Ferreira, L.M.A. 1999. Homologous xylanases from Clostridium thermocellum: evidence for bi-functional activity, synergism between xylanase catalytic modules and the presence of xylan-binding domains in enzyme complexes. Biochem. J. 342: 105-110.
8. 14: sequence relationships, synergistic interactions, and oxygen sensitivity of a novel enzyme with exoxylanase and beta-(1-4)-xylosidase activities. Appl. Environ. Microbiol. 61: 2958-2964.
9. Ghangas, G.S., Hu, Y.J., and Wilson, D.B. 1989. Cloning of a Thermomonosora fusca xylanase gene and its expression in Escherichia coli and Streptomyces lividans J. Bacteriol. 171: 2963-2969.
10. Ghose, T.K. 1987. Measurement of cellulase activities. Pure and Appl. Chem. 59: 257-268.
11. Hägerdahl, B.G.R., Ferchak, J.D., and Pye, E.K. 1978. Cellulolytic enzyme system of Thermomonospora sp. grown on microcrystalline cellulose. Appl. Envir. Microbiol. 36: 606-612.
12. Hespell, R.B., and Cotta, M.A. 1995. Degradation and utilization by Butyrivibrio fibrisolvens H17c of xylans with different chemical and physical properties. Appl. Environ. Microbiol. 61: 3042-3050.
13. Irwin, D., Jung, E., and Wilson, D.B. 1994. Characterization and sequence of a Thermomonospora fusca xylanase. Appl. Environ. Microbiol. 60: 763-770.
14. Irwin, D., Cheng, M., Xiang, B., Rose, J.K.C., and Wilson, D.B. 2003a. Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca. Eur. J. Biochem. 270: 3083-3091.
15. Irwin, D., Leathers, T.D., Greene, R.V., and Wilson, D.B. 2003b. Corn fiber hydrolysis by Thermobifida fusca extracellular enzymes. Appl. Microbiol. Biotechnol. 61: 352-358.
16. Jendrossek, D., Muller, B., and Schlegel, H.G. 1993. Cloning and characterization of the poly(hydroxalkanoic acid)-depolymerase gene locus, phaZ1, of Pseudomonas lemoignei and its gene product. Eur. J. Biochem. 218: 701-710.
17. Joseleau, J.P., Comtat, J., and Ruel, K. 1992. Chemical structure of xylans and their interaction in the plant cell walls. In Xylan and xylanases. Edited by J. Visser, G. Beldman, M.A. Kusters-van Someren, and A.G.J. Voragen. Elsevier, London, pp. 1-15.
18. Kroon, P.A., Williamson, G., Fish, N.M., Archer, D.B., and Belshaw, N.J. 2000. A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module. Eur. J. Biochem. 267: 6740-6752.
19. Kulkarni, N., Shendye, A., and Rao, M. 1999. Molecular and biotechnological aspects of xylanases. FEMS Microbiol. Rev. 23: 411-456.
20. Laemmli, U.K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London), 227: 680-685.
21. Lever, M. 1972. A new reaction for colorimetric determination of carbohydrates. Anal. Biochem. 47: 273-279.
22. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
23. Millward-Sadler, S.J., Poole, D.M., Henrissat, B., Hazlewood, G.P., Clarke, J.H., and Gilbert, H.J. 1994. Evidence for a general role for high-affinity non-catalytic cellulose binding domains in microbial plant cell wall hydrolases. Mol. Microbiol. 11: 375-382.
24. Saito, T., Suzuki, K., Yamamoto, J., Fukui, T., Miwa, K., Tomita, K. et al. 1989. Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis. J. Bacteriol. 171: 184-189.
25. Sambrook, J., and Russel, D.B. 2001. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Spiridonov, N.A., and Wilson, D.B. 1999. Characterization and cloning of CelR, a transcriptional regulator of cellulase genes from Thermomonospora fusca. J. Biol. Chem. 274: 13 127-13 132.
27. Tuncer, M., and Ball, A.S. 2003. Co-operative actions and degradation analysis of purified xylan-degrading enzymes from Thermomonospora fusca BD25 on oat-spelt xylan J. Appl. Microbiol. 94: 1030-1035.
28. Wilson, D.B., and Irwin, D.C. 1999. Genetics and properties of cellulases. In Advances in biochemical engineering biotechnology: recent progress in bioconversion of lignocellulosics. Edited by G.T. Tsao and T. Scheper. Springer-Verlag. pp. 1-21.