Thermobifida fusca exoglucanase Cel6B is incompatible with the cellulosomal mode in contrast to endoglucanase Cel6A

Systems and Synthetic Biology

Volumn 4, Issue 3, 2010, Pages 193-201

  Caspi, Jonathan ^a   Barak, Yoav ^a   Haimovitz, Rachel ^a   Gilary, Hadar ^a   Irwin, Diana C ^b   Lamed, Raphael ^c   Wilson, David B ^b   Bayer, Edward A ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

^b Department of Obstetrics Gynecology   (United States)

^c TEL AVIV UNIVERSITY   (Israel)

Author keywords

Bioenergy; Cellulases; Degradation of crystalline cellulose; Designer cellulosome; Enzyme synergy; Substrate targeting and enzyme proximity

Indexed keywords

BACTERIA (MICROORGANISMS); THERMOBIFIDA FUSCA;

EID: 77958497861     PISSN: 18725325     EISSN: 18725333     Source Type: Journal    
DOI: 10.1007/s11693-010-9056-1     Document Type: Article

References (35)

1. Barak Y, Handelsman T, Nakar D, Mechaly A, Lamed R, Shoham Y, Bayer EA (2005) Matching fusion-protein systems for affinity analysis of two interacting families of proteins: the cohesin-dockerin interaction. J Mol Recogit 18: 491-501.
2. Bayer EA, Morag E, Lamed R (1994) The cellulosome-a treasure-trove for biotechnology. Trends Biotechnol 12: 378-386.
3. Bayer EA, Belaich J-P, Shoham Y, Lamed R (2004) The cellulosomes: multi-enzyme machines for degradation of plant cell wall polysaccharides. Annu Rev Microbiol 58: 521-554.
4. Bayer EA, Lamed R, Himmel ME (2007) The potential of cellulases and cellulosomes for cellulosic waste management. Curr Opin Biotechnol 18: 237-245.
5. Bayer EA, Henrissat B, Lamed R (2008a) The cellulosome: a natural bacterial strategy to combat biomass recalcitrance. In: Himmel ME (ed) Biomass recalcitrance. Blackwell, London, pp 407-426.
6. Bayer EA, Shoham Y, Lamed R (2008b) Cellulosome-enhanced conversion of biomass: on the road to bioethanol. In: Wall J, Harwood C, Demain AL (eds) Bioengergy. ASM Press, Washington, DC, pp 75-96.
7. v-cellulose binding domain fusion proteins. Protein Expr Purif 17: 249-259.
8. Caspi J, Irwin D, Lamed R, Shoham Y, Fierobe H-P, Wilson DB, Bayer EA (2006) Thermobifida fusca family-6 cellulases as potential designer cellulosome components. Biocat Biotransform 24: 3-12.
9. Caspi J, Irwin D, Lamed R, Fierobe H-P, Wilson DB, Bayer EA (2008) Conversion of noncellulosomal Thermobifida fusca free exoglucanases into cellulosomal components: comparative impact on cellulose-degrading activity. J Biotechnol 135: 351-357.
10. Caspi J, Barak Y, Haimovitz R, Irwin D, Lamed R, Wilson DB, Bayer EA (2009) Conversion of a Thermobifida fusca endoglucanase into the cellulosomal mode: effect of linker length and dockerin position. Appl Environ Microbiol 75: 7335-7342.
11. Demain AL, Newcomb M, Wu JH (2005) Cellulase, clostridia, and ethanol. Microbiol Mol Biol Rev 69: 124-154.
12. Ding S-Y, Bayer EA, Steiner D, Shoham Y, Lamed R (2000) A scaffoldin of the Bacteroides cellulosolvens cellulosome that contains 11 type II cohesins. J Bacteriol 182: 4915-4925.
13. Doi RH, Kosugi A (2004) Cellulosomes: plant-cell-wall-degrading enzyme complexes. Nat Rev Microbiol 2: 541-551.
14. Dror TW, Morag E, Rolider A, Bayer EA, Lamed R, Shoham Y (2003a) Regulation of the cellulosomal celS (cel48A) gene of Clostridium thermocellum is growth-rate dependent. J Bacteriol 185: 3042-3048.
15. Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y (2003b) Regulation of expression of scaffoldin-related genes in Clostridium thermocellum. J Bacteriol 185: 5109-5116.
16. Dror TW, Rolider A, Bayer EA, Lamed R, Shoham Y (2005) Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. J Bacteriol 187: 2261-2266.
17. Fierobe H-P, Mechaly A, Tardif C, Belaich A, Lamed R, Shoham Y, Belaich J-P, Bayer EA (2001) Design and production of active cellulosome chimeras: selective incorporation of dockerin-containing enzymes into defined functional complexes. J Biol Chem 276: 21257-21261.
18. Fierobe H-P, Bayer EA, Tardif C, Czjzek M, Mechaly A, Belaich A, Lamed R, Shoham Y, Belaich J-P (2002) Degradation of cellulose substrates by cellulosome chimeras: substrate targeting versus proximity of enzyme components. J Biol Chem 277: 49621-49630.
19. Fierobe H-P, Mingardon F, Mechaly A, Belaich A, Rincon MT, Lamed R, Tardif C, Belaich J-P, Bayer EA (2005) Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined tri-functional scaffoldin. J Biol Chem 280: 16325-16334.
20. Ghangas GS, Wilson DB (1988) Cloning of the Thermomonospora fusca endoglucanase E2 gene in Streptomyces lividans: affinity purification and functional domains of the cloned gene product. Appl Environ Microbiol 54: 2521-2526.
21. Haimovitz R, Barak Y, Morag E, Voronov-Goldman M, Lamed R, Bayer EA (2008) Cohesin-dockerin microarray: diverse specificities between two complementary families of interacting protein modules. Proteomics 8: 968-979.
22. Irwin DC, Zhang S, Wilson DB (2000) Cloning, expression and characterization of a family 48 exocellulase, Cel48A, from Thermobifida fusca. Eur J Biochem 267: 4988-4997.
23. Jindou S, Kajino T, Inagaki M, Karita S, Beguin P, Kimura T, Sakka K, Ohmiya K (2004) Interaction between a type-II dockerin domain and a type-II cohesin domain from Clostridium thermocellum cellulosome. Biosci Biotechnol Biochem 68: 924-926.
24. Mechaly A, Yaron S, Lamed R, Fierobe H-P, Belaich A, Belaich J-P, Shoham Y, Bayer EA (2000) Cohesin-dockerin recognition in cellulosome assembly: experiment versus hypothesis. Proteins 39: 170-177.
25. Mechaly A, Fierobe H-P, Belaich A, Belaich J-P, Lamed R, Shoham Y, Bayer EA (2001) Cohesin-dockerin interaction in cellulosome assembly: a single hydroxyl group of a dockerin domain distinguishes between non-recognition and high-affinity recognition. J Biol Chem 276: 9883-9888 Erratum 19678.
26. Mingardon F, Chanal A, López-Contreras AM, Dray C, Bayer EA, Fierobe H-P (2007a) Incorporation of fungal cellulases in bacterial minicellulosomes yields viable, synergistically acting cellulolytic complexes. Appl Environ Microbiol 73: 3822-3832.
27. Mingardon F, Chanal A, Tardif C, Bayer EA, Fierobe H-P (2007b) Exploration of new geometries in cellulosome-like chimeras. Appl Environ Microbiol 73: 7138-7149.
28. Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y (1995) Expression, purification and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl Environ Microbiol 61: 1980-1986.
29. Newcomb M, Chen CY, Wu JH (2007) Induction of the celC operon of Clostridium thermocellum by laminaribiose. Proc Natl Acad Sci USA 104: 3747-3752.
30. Pagès S, Belaich A, Belaich J-P, Morag E, Lamed R, Shoham Y, Bayer EA (1997) Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridiumcellulolyticum: prediction of specificity determinants of the dockerin domain. Proteins 29: 517-527.
31. Teeri TT (1997) Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol 15: 160-167.
32. Wilson DB (2004) Studies of Thermobifida fusca plant cell wall degrading enzymes. Chem Rec 4: 72-82.
33. Wilson DB (2009) Cellulases and biofuels. Curr Opin Biotechnol 20: 295-299.
34. Yaron S, Morag E, Bayer EA, Lamed R, Shoham Y (1995) Expression, purification and subunit-binding properties of cohesins 2 and 3 of the Clostridium thermocellum cellulosome. FEBS Lett 360: 121-124.
35. Zhang S, Lao G, Wilson DB (1995) Characterization of a Thermomonospora fusca exocellulase. Biochemistry 34: 3386-3395.