Astragalus polysaccharide stimulates glucose uptake in L6 myotubes through AMPK activation and AS160/TBC1D4 phosphorylation

Acta Pharmacologica Sinica

Volumn 34, Issue 1, 2013, Pages 137-145

  Liu, Jian ^a   Zhang, Jing Fang ^b   Lu, Jin Zhi ^a   Zhang, De Ling ^a   Li, Ke ^a   Su, Ke ^a   Wang, Jing ^a   Zhang, Ye Min ^a   Wang, Nian ^a   Yang, Si Tu ^a   Bu, Lang ^a   Ou Yang, Jing Ping ^a  

^a WUHAN UNIVERSITY   (China)

^b JINGCHU UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

AMP; AMPK; AS160; Astragalus polysaccharide; CaMKK ; glucose uptake; L6 myotubes; LKB1; type 2 diabetes mellitus

Indexed keywords

ADENINE NUCLEOTIDE; ASTRAGALUS MEMBRANACEUS EXTRACT; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE KINASE; CALCIUM CALMODULIN DEPENDENT PROTEIN KINASE KINASE BETA; HYDROXYMETHYLGLUTARYL COENZYME A REDUCTASE KINASE; POLYSACCHARIDE; PROTEIN KINASE B; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; ASTRAGALUS MEMBRANACEUS; CONTROLLED STUDY; ENZYME PHOSPHORYLATION; GENE TRANSFER; GENETIC TRANSFECTION; GLUCOSE TRANSPORT; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MYOTUBE; NONHUMAN; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATE; AMP-ACTIVATED PROTEIN KINASES; ANIMALS; ASTRAGALUS PLANT; BIOLOGICAL TRANSPORT; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE; CELL LINE; ENZYME ACTIVATION; GLUCOSE; GTPASE-ACTIVATING PROTEINS; MUSCLE FIBERS, SKELETAL; PHOSPHORYLATION; POLYSACCHARIDES; RATS; UP-REGULATION;

EID: 84872092574     PISSN: 16714083     EISSN: 17457254     Source Type: Journal    
DOI: 10.1038/aps.2012.133     Document Type: Article

References (32)

1. De Fronzo RA, Jacot E, Jequier E, Maeder E, Wahren J, Felber JP. The effect of insulin on the disposal of intravenous glucose. Results from indirect calorimetry and hepatic and femoral venous catheterization. Diabetes 1981;30:1000-7.
2. Dohm GL, Tapscott EB, Pories WJ, Dabbs DJ, Flickinger EG, Meelheim D, et al. An in vitro human muscle preparation suitable for metabolic studies. Decreased insulin stimulation of glucose transport in muscle from morbidly obese and diabetic subjects. J Clin Invest 1988;82:486-94.
3. De Fronzo RA, Bonadonna RC, Ferrannini E. Pathogenesis of NIDDM. A balanced overview. Diabetes Care 1992;15:318-68.
4. Misra P, Chakrabarti R. The role of AMP kinase in diabetes. Indian J Med Res 2007;125:389-98. (Pubitemid 46805453)
5. Hardie DG, Scott JW, Pan DA, Hudson ER. Management of cellular energy by the AMP-activated protein kinase system. FEBS Lett 2003;546:113-20. (Pubitemid 36782291)
6. Hawley SA, Davison M, Woods A, Davies SP, Beri RK, Carling D, et al. Characterization of the AMP-activated protein kinase kinase from rat liver and identification of threonine 172 as the major site at which it phosphorylates AMP-activated protein kinase. J Biol Chem 1996;271:27879-87. (Pubitemid 26367365)
7. Stein SC, Woods A, Jones NA, Davison MD, Carling D. The regulation of AMP-activated protein kinase by phosphorylation. Biochem J 2000;345:437-43. (Pubitemid 30099070)
8. Hardie DG. AMPK: a key regulator of energy balance in the single cell and the whole organism. Int J Obes (Lond) 2008;32:S7-12.
9. Merrill GF, Kurth EJ, Hardie DG, Winder WW. AICA riboside increases AMP-activated protein kinase, fatty acid oxidation, and glucose uptake in rat muscle. Am J Physiol 1997;273:E1107-12.
10. Sakamoto K, Holman GD. Emerging role for AS160/TBC1D4 and TBC1D1 in the regulation of GLUT4 traffic. Am J Physiol Endocrinol Metab 2008;295:E29-37.
11. Zerial M, McBride H. Rab proteins as membrane organizers. Nat Rev Mol Cell Biol 2001;2:107-17. (Pubitemid 33674039)
12. Sano H, Eguez L, Teruel MN, Fukuda M, Chuang TD, Chavez JA, et al. Rab10, a target of the AS160 Rab GAP, is required for insulinstimulated translocation of GLUT4 to the adipocyte plasma membrane. Cell Metab 2007;5:293-303. (Pubitemid 46477855)
13. Zeigerer A, McBrayer MK, McGraw TE. Insulin stimulation of GLUT4 exocytosis, but not its inhibition of endocytosis, is dependent on RabGAP AS160. Mol Biol Cell 2004;15:4406-15. (Pubitemid 39299091)
14. Karlsson HK, Zierath JR, Kane S, Krook A, Lienhard GE, Wallberg-Henriksson H. Insulin-stimulated phosphorylation of the Akt substrate AS160 is impaired in skeletal muscle of type 2 diabetic subjects. Diabetes 2005;54:1692-7. (Pubitemid 40770759)
15. Wang N, Zhang DL, Mao XQ, Zou F, Jin H, Ou-Yang JP. Astragalus polysaccharides decreased the expression of PTP1B through relieving ER stress induced activation of ATF6 in a rat model of type 2 diabetes. Mol Cell Endocrinol 2009;307:89-98.
16. Zou F, Mao XQ, Wang N, Liu J, Ou-Yang JP. Astragalus polysaccharides alleviates glucose toxicity and restores glucose homeostasis in diabetic states via activation of AMPK. Acta Pharmacol Sin 2009;30:1607-15.
17. Tanishita T, Shimizu Y, Minokoshi Y, Shimazu T. The beta3-adrenergic agonist BRL37344 increases glucose transport into L6 myocytes through a mechanism different from that of insulin. J Biochem 1997;122:90-5. (Pubitemid 27346226)
18. Hutchinson DS, Bengtsson T. alpha1A-adrenoceptors activate glucose uptake in L6 muscle cells through a phospholipase C-, phosphatidylinositol-3. kinase-, and atypical protein kinase C-dependent pathway. Endocrinology 2005;146:901-12. (Pubitemid 40129848)
19. Zou MH, Hou XY, Shi CM, Kirkpatick S, Liu F, Goldman MH, et al. Activation of 5'-AMP-activated kinase is mediated through c-Src and phosphoinositide 3-kinase activity during hypoxia-reoxygenation of bovine aortic endothelial cells. Role of peroxynitrite. J Biol Chem 2003;278:34003-10. (Pubitemid 37553236)
20. Hahn-Windgassen A, Nogueira V, Chen CC, Skeen JE, Sonenberg N, Hay N. Akt activates the mammalian target of rapamycin by regulating cellular ATP level and AMPK activity. J Biol Chem 2005;280:32081-9. (Pubitemid 41361813)
21. Sano H, Kane S, Sano E, Miinea CP, Asara JM, Lane WS, et al. Insulinstimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation. J Biol Chem 2003;278:14599-602. (Pubitemid 36799780)
22. Hardie DG. Minireview: the AMP-activated protein kinase cascade: the key sensor of cellular energy status. Endocrinology 2003;144:5179-83. (Pubitemid 37476044)
23. Bruss MD, Arias EB, Lienhard GE, Cartee GD. Increased phosphorylation of Akt substrate of 160 kDa (AS160) in rat skeletal muscle in response to insulin or contractile activity. Diabetes 2005;54:41-50. (Pubitemid 40105093)
24. Treebak JT, Glund S, Deshmukh A, Klein DK, Long YC, Jensen TE, et al. AMPK-mediated AS160 phosphorylation in skeletal muscle is dependent on AMPK catalytic and regulatory subunits. Diabetes 2006;55:2051-8. (Pubitemid 44509979)
25. Suter M, Riek U, Tuerk R, Schlattner U, Wallimann T, Neumann D. Dissecting the role of 5'-AMP for allosteric stimulation, activation, and deactivation of AMP-activated protein kinase. J Biol Chem 2006;281:32207-16. (Pubitemid 46036775)
26. Russell RR 3rd, Bergeron R, Shulman GI, Young LH. Translocation of myocardial GLUT-4 and increased glucose uptake through activation of AMPK by AICAR. Am J Physiol 1999;277:H643-9. (Pubitemid 29415978)
27. Wu Y, Ou-Yang JP, Wu K, Wang Y, Zhou YF, Wen CY. Hypoglycemic effect of Astragalus polysaccharide and its effect on PTP1B. Acta Pharmacol Sin 2005;26:345-52. (Pubitemid 40388072)
28. Yeh LA, Lee KH, Kim KH. Regulation of rat liver acetyl-CoA carboxylase. Regulation of phosphorylation and inactivation of acetyl-CoA carboxylase by the adenylate energy charge. J Biol Chem 1980;255:2308-14. (Pubitemid 10036033)
29. Scott JW, Hawley SA, Green KA, Anis M, Stewart G, Scullion GA, et al. CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations. J Clin Invest 2004;113:274-84. (Pubitemid 38544212)
30. Davies SP, Helps NR, Cohen PT, Hardie DG. 5'-AMP inhibits dephosphorylation, as well as promoting phosphorylation, of the AMPactivated protein kinase. Studies using bacterially expressed human protein phosphatase-2C alpha and native bovine protein phosphatase-2AC. FEBS Lett 1995;377:421-5. (Pubitemid 26027159)
31. Wu Y, Song P, Xu J, Zhang M, Zou MH. Activation of protein phosphatase 2A by palmitate inhibits AMP-activated protein kinase. J Biol Chem 2007;282:9777-88. (Pubitemid 47104626)
32. Kramer HF, Witczak CA, Fujii N, Jessen N, Taylor EB, Arnolds DE, et al. Distinct signals regulate AS160 phosphorylation in response to insulin, AICAR, and contraction in mouse skeletal muscle. Diabetes 2006;55:2067-76. (Pubitemid 44509981)