Electron microscopy of legionella and legionella-infected cells

Methods in Molecular Biology

Volumn 954, Issue , 2013, Pages 279-307

  Faulkner, Gary ^a   Garduño, Rafael A ^b  

^a DALHOUSIE UNIVERSITY   (Canada)

^b Centre for Clinical Research   (Canada)

Author keywords

Cell morphology; Fixation; Immuno gold; Negative stain; Ultrastructure

Indexed keywords

LEGIONELLA;

EID: 84872086015     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-161-5_17     Document Type: Article

References (15)

1. Rodgers FG, Macrae AD, Lewis MJ (1978) Electron microscopy of the organism of Legionnaires' disease. Nature 272:825-826
2. Rodgers FG (1979) Ultrastructure of Legionella pneumophila . J Clin Pathol 32:1195-1202
3. Gress FM, Myerowitz RL, Pascule AW, Rinaldo CR Jr, Dowling JN (1980) The ultrastructural morphologic features of Pittsburgh pneumonia agent. Am J Pathol 101:63-78
4. Horwitz MA (1983) Formation of a novel phagosome by the Legionnaires' disease bacterium (Legionella pneumophila) in human monocytes. J Exp Med 158:1319-1331
5. Horwitz MA (1983) The Legionnaires' disease bacterium (Legionella pneumophila) inhibits phagosome-lysosome fusion in human monocytes. J Exp Med 158:2108-2126
6. Garduño RA, Quinn F, Hoffman PS (1998) HeLa cells as a model to study the invasiveness and biology of Legionella pneumophila . Can J Microbiol 44:430-440
7. Faulkner G, Garduño RA (2002) Ultrastructural analysis of differentiation in Legionella pneumophila . J Bacteriol 184:7025-7041
8. Garduño RA, Garduño E, Hiltz M, Hoffman PS (2002) Intracellular growth of Legionella pneumophila gives rise to a differentiated form dissimilar to stationary phase forms. Infect Immun 70:6273-6283
9. Garduño RA, Faulkner G, Trevors MA, Vats N, Hoffman PS (1998) Immunolocalization of Hsp60 in Legionella pneumophila . J Bacteriol 180:505-513
10. Haniachi T, Sato T, Iwamoto T, Malavashi-Yamashiro Y, Hoshino M, Mizuno N (1986) A stable stain by modi fi cation of Sato's method. J Electron Microsc 35:304-306
11. Molmeret M, Bitar DM, Han LH, Abu Kwaik Y (2004) Disruption of the phagosomal membrane and egress of Legionella pneumophila into the cytoplasm during the last stages of intracellular infection of macrophages and Acanthamoeba polyphaga . Infect Immun 72:4040-4051
12. Molmeret M, Marina S, Rexford A, Carabeo RA, Abu Kwaik Y (2007) Rapid escape of the dot/icm mutants of Legionella pneumophila into the cytosol of mammalian and protozoan cells. Infect Immun 75:3290-3304
13. Pease DC (1964) Histological techniques for electron microscopy. Academic, London
14. Hayat MA (1970) Principles and techniques of electron microscopy - biological applications, vol 1. Van Nostrand Reinhold Company, New York
15. Dawes CJ (1971) Biological techniques in electron microscopy. Barnes & Noble, New York