Immunolocalization of Hsp60 in Legionella pneumophila

Journal of Bacteriology

Volumn 180, Issue 3, 1998, Pages 505-513

  Garduño, Rafael A ^a,b   Faulkner, Gary ^a   Trevors, Mary A ^a   Vats, Neeraj ^a   Hoffman, Paul S ^a,b  

^a DALHOUSIE UNIVERSITY   (Canada)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; CHAPERONIN; HEAT SHOCK PROTEIN 60; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY;

ANTIBODY LABELING; ARTICLE; BACTERIAL GROWTH; BACTERIAL MEMBRANE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; LEGIONELLA PNEUMOPHILA; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; PROTEIN SECRETION;

EID: 0031910013     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.3.505-513.1998     Document Type: Article

References (57)

1. Abu Kwaik, Y., B. I. Eisenstein, and N. C. Engleberg. 1993. Phenotypic modulation by Legionella pneumophila upon infection of macrophages. Infect. Immun. 61:1320-1329.
2. Abu Kwaik, Y., L.-Y. Gao, O. S. Harb, and B. J. Stone. 1997. Transcriptional regulation of the macrophage-induced gene (gspA) of Legionella pneumophila and phenotypic characterization of a null mutant. Mol. Microbiol. 24:629-642.
3. Baumann, P., N. A. Moran, and L. Baumann. 1997. The evolution and genetics of aphid endosymbionts. BioScience 47:12-20.
4. Berryman, M. A., and R. D. Rodewald. 1990. An enhanced method for post-embedding immunocytochemical staining which preserves cell membranes. J. Histochem. Cytochem. 38:159-170.
5. Blander, S. J., and M. A. Horwitz. 1993. Major cytoplasmic membrane protein of Legionella pneumophila, a genus common antigen and member of the hsp 60 family of heat shock proteins, induces protective immunity in a guinea pig model of Legionnaires' disease. J. Clin. Invest. 91:717-723.
6. Butler, C. A., and P. S. Hoffman. 1990. Characterization of a major 31-kilodalton peptidoglycan-bound protein of Legionella pneumophila. J. Bacteriol. 172:2401-2407.
7. Butler, C. A., E. D. Street, T. P. Hatch, and P. S. Hoffman. 1985. Disulfide-bonded outer membrane proteins in the genus Legionella. Infect. Immun. 48:14-18.
8. Ciesielski, C. A., M. J. Blaser, and W.-L. L. Wang. 1986. Serogroup specificity of Legionella pneumophila is related to lipopolysaccharide characteristics. Infect. Immun. 51:397-404.
9. Craig, E. A., B. D. Gambill, and R. J. Nelson. 1993. Heat-shock proteins: molecular chaperones of protein biogenesis. Microbiol. Rev. 57:402-414.
10. Dunn, B. E., N. B. Vakil, B. G. Schneider, M. M. Miller, J. B. Zitzer, T. Peutz, and S. H. Phadnis. 1997. Localization of Helicobacter pylori urease and heat shock protein in human gastric biopsies. Infect. Immun. 65:1181-1188.
11. Ensgraber, M., and M. Loos. 1992. A 66-kilodalton heat shock protein of Salmonella typhimurium is responsible for binding of the bacterium to intestinal mucus. Infect. Immun. 60:3072-3078.
12. Faulkner, G. Unpublished data.
13. Fernandez, R. C., S. M. Logan, S. H. S. Lee, and P. S. Hoffman. 1946 Elevated levels of Legionella pneumophila stress protein Hsp60 early in infection of human monocytes and L929 cells correlate with virulence. Infect Immun. 64:1968-1976.
14. Fields, B. S. 1996. The molecular ecology of legionellae. Trends Microbiol. 4:286-290.
15. Frydman, J., and F.-U. Hartl. 1994. Molecular chaperone functions of hsp70 and hsp60 in protein folding, p. 251-283. In R. I. Morimoto, A. Tissières, and C. Georgopoulos (ed.), The biology of heat shock proteins and molecular chaperones. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
16. Gabay, J. E., and M. A. Horwitz. 1985. Isolation and characterization of the cytoplasmic and outer membranes of the Legionnaires' disease bacterium Legionella pneumophila. J. Exp. Med. 161:409-422.
17. Garduno, R. A., and P. S. Hoffman. Unpublished data.
18. Garduno, R. A., F. D. Quinn, and P. S. Hoffman. HeLa cells as a model to study the invasiveness and biology of Legionella pneumophila. Submitted for publication.
19. Gillis, T. P., R. A. Miller, D. B. Young, S. R. Khanolkar, and T. M. Buchanan. 1985. Immunochemical characterization of a protein associated with Mycobacterium leprae cell wall. Infect. Immun. 49:371-377.
20. Gupta, R. S. 1995. Evolution of the chaperonin families (Hsp60, Hsp10 and Tcp-1) of proteins and the origin of eukaryotic cells. Mol. Microbiol. 15:1-11.
21. Hanaichi, T., T. Sato, T. Iwamoto, Y. Malavasi-Yamashiro, M. Hoshino, and N. Mizuno. 1986. A stable stain by modification of Sato's method. J. Electron Microsc. 35:304-306.
22. Helsel, L. O., W. F. Bibb, C. A. Butler, P. S. Hoffman, and R. M. McKinney. 1988. Recognition of a genus-wide antigen of Legionella by a monoclonal antibody. Curr. Microbiol. 16:201-208.
23. Hoffman, P. S., C. A. Butler, and F. D. Quinn. 1989. Cloning and temperature-dependent expression in Escherichia coli of a Legionella pneumophila gene coding for a genus-common 60-kilodalton antigen. Infect. Immun. 57:1731-1739.
24. Hoffman, P. S., L. Houston, and C. A. Butler. 1990. Legionella pneumophila htpAB heat shock operon: nucleotide sequence and expression of the 60-kilodalton antigen in L. pneumophila-infected HeLa cells. Infect. Immun. 58:3380-3387.
25. Huesca, M., S. Borgia, P. Hoffman, and C. A. Lingwood. 1996. Acidic pH changes receptor binding specificity of Helicobacter pylori: a binary adhesion model in which surface heat shock (stress) proteins mediate sulfatide recognition in gastric colonization. Infect. Immun. 64:2643-2648.
26. Jäger, K. M., and B. Bergman. 1990. Localization of a multifunctional chaperonin (GroEL protein) in nitrogen-fixing Anabaena PCC 7120. Planta 183:120-125.
27. Kakeda, K., and H. Ishikawa. 1991. Molecular chaperon produced by an intracellular symbiont. J. Biochem. 110:583-587.
28. Kessler, F., and G. Blobel. 1996. Interaction of the protein import and folding machineries in the chloroplast. Proc. Natl. Acad. Sci. USA 93:7684-7689.
29. Kovács, E., Z. Török, I. Horváth, and L. Vígh. 1994. Heat stress induces association of the GroEL analog chaperonin with thylakoid membranes in cyanobacterium, Synechocystis PCC 6803. Plant Physiol. Biochem. 32:285-293.
30. Krishnasamy, S., R. M. Mannan, M. Krishnan, and A. Gnanam. 1988. Heat shock response of the chloroplast genome in Vigna sinensis. J. Biol. Chem. 263:5104-5109.
31. Kubota, H., G. Hynes, and K. Willison. 1995. The chaperonin containing t-complex polypeptide 1 (TCP-1). Multisubunit machinery assisting in protein folding and assembly in the eukaryotic cytosol. Eur. J. Biochem. 230:3-16.
32. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
33. Lema, M. W., A. Brown, C. A. Butler, and P. S. Hoffman. 1988. Heat-shock response in Legionella pneumophila. Can. J. Microbiol. 34:1148-1153.
34. McFadden, B. A., J. A. Torres-Ruiz, and V. R. Franceschi. 1989. Localization of ribulose-bisphosphate carboxylase-oxygenase and its putative binding protein in the cell envelope of Chromatium vinosum. Planta 178:297-302.
35. Missiakas, D., and S. Raina. 1997. Protein folding in the bacterial periplasm. J. Bacteriol. 179:2465-2471.
36. Ohtaka, C., H. Nakamura, and H. Ishikawa. 1992. Structures of chaperonins from an intracellular symbiont and their functional expression in Escherichia coli groE mutants. J. Bacteriol. 174:1869-1874.
37. Parsell, D. A., and S. Lindquist. 1993. The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu. Rev. Genet. 27:437-496.
38. Pascule, A. W., J. C. Feeley, R. J. Gibson, L. G. Cordes, R. L. Meyerowitz, C. M. Patton, G. W. Gorman, C. L. Carmack, J. W. Ezzell, and J. N. Dowling. 1980. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J. Infect. Dis. 141:727-732.
39. Pau, C.-P., B. B. Plikaytis, G. M. Carlone, and I. M. Warner. 1988. Purification, partial characterization, and seroreactivity of a genuswide 60-kilodalton Legionella protein antigen. J. Clin. Microbiol. 26:67-71.
40. Phadnis, S. H., M. H. Parlow, M. Levy, D. Ilver, C. M. Caulkins, J. B. Connors, and B. E. Dunn. 1996. Surface localization of Helicobacter pylori urease and a heat shock protein homolog requires bacterial autolysis. Infect. Immun. 64:905-912.
41. Plikaytis, B. B., G. M. Carlone, C.-P. Pau, and H. W. Wilkinson. 1987. Purified 60-kilodalton Legionella protein antigen with Legionella-specific and nonspecific epitopes. J. Clin. Microbiol. 25:2080-2084.
42. Retzlaff, C., Y. Yamamoto, P. S. Hoffman, H. Friedman, and T. W. Klein. 1994. Bacterial heat-shock proteins induce cytokine mRNA and interleukin-1 secretion in macrophage cultures. Infect. Immun. 62:5689-5693.
43. Retzlaff, C., Y. Yamamoto, S. Okubo, P. S. Hoffman, H. Friedman, and T. W. Klein. 1996. Legionella pneumophila heat-shock protein-induced increase of interleukin-1β mRNA involves protein kinase C signaling in macrophages. Immunology 89:281-288.
44. Sampson, J. S., B. B. Plikaytis, and H. W. Wilkinson. 1986. Immunologic response of patients with legionellosis against major protein-containing antigens of Legionella pneumophila serogroup 1 as shown by immunoblot analysis. J. Clin. Microbiol. 23:92-99.
45. Scorpio, A., P. Johnson, A. Laquerre, and D. R. Nelson. 1994. Subcellular localization and chaperone activities of Borrelia burgdorferi Hsp60 and Hsp70. J. Bacteriol. 176:6449-6456.
46. Soltys, B. J., and R. S. Gupta. 1996. Immunoelectron microscopic localization of the 60-kDa heat shock chaperonin protein (Hsp60) in mammalian cells. Exp. Cell Res. 222:16-27.
47. Soltys, B. J., and R. S. Gupta. 1997. Cell surface localization of the 60 kDa heat shock chaperonin protein (hsp60) in mammalian cells. Cell Biol. Int. 21:315-320.
48. Steinmetz, I., C. Rheinheimer, I. Hübner, and D. Bitter-Suermann. 1991. Genus-specific epitope on the 60-kilodalton Legionella heat shock protein recognized by a monoclonal antibody. J. Clin. Microbiol. 29:346-354.
49. Susa, M., J. Hacker, and R. Marre. 1996. De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells. Infect. Immun. 64:1679-1684.
50. Török, Z., I. Horváth, P. Goloubinoff, E. Kovács, A. Glatz, G. Balogh, and L. Vígh. 1997. Evidence for a lipochaperonin: association of active protein-folding GroESL oligomers with lipids can stabilize membranes under heat shock conditions. Proc. Natl. Acad. Sci. USA 94:2192-2197.
51. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
52. Trent, J. D., H. K. Kagawa, T. Yaoi, E. Olle, and N. J. Zaluzec. 1997. Chaperonin filaments: the archaeal cytoskeleton? Proc. Natl. Acad. Sci. USA 94:5383-5388.
53. van den Heuvel, J. F. J. M., M. Verbeek, and F. van der Wilk. 1994. Endosymbiotic bacteria associated with circulative transmission of potato leafroll virus by Myzus persicae. J. Gen. Virol. 75:2559-2565.
54. Vodkin, M. H., and J. C. Williams. 1988. A heat shock operon in Coxiella burnetii produces a major antigen homologous to a protein in both mycobacteria and Escherichia coli. J. Bacteriol. 170:1227-1234.
55. Weeratna, R., D. A. Stamler, P. H. Edelstein, M. Ripley, T. Marrie, D. Hoskin, and P. S. Hoffman. 1994. Human and guinea pig immune responses to Legionella pneumophila protein antigens OmpS and Hsp60. Infect. Immun. 62:3454-3462.
56. Willison, K. R., and H. Kubota. 1994. The structure, function, and genetics of the chaperonin containing TCP-1 (CCT) in eukaryotic cytosol, p. 299-312. In R. I. Morimoto, A. Tissières, and C. Georgopoulos (ed.), The biology of heat shock proteins and molecular chaperones. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
57. Yamaguchi, H., T. Osaki, H. Taguchi, T. Hanawa, T. Yamamoto, and S. Kamiya. 1996. Induction and epitope analysis of Helicobacter pylori heat shock protein. J. Gastroenterol. 31:12-15.