HeLa cells as a model to study the invasiveness and biology of Legionella pneumophila

Canadian Journal of Microbiology

Volumn 44, Issue 5, 1998, Pages 430-440

  Garduño, Rafael A ^b   Quinn, Frederick D ^a,b   Hoffman, Paul S ^b  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b DALHOUSIE UNIVERSITY   (Canada)

Author keywords

HeLa cells; Intracellular pathogens; Invasion mechanisms; Legionella pneumophila

Indexed keywords

BACTERIAL PROTEIN; GALACTOSE; GLUCOSE;

ACTIN POLYMERIZATION; ARTICLE; BACTERIAL INFECTION; BACTERIAL VIRULENCE; CELL ADHESION; CELL CULTURE; CELL DIVISION; CELL INVASION; CONTROLLED STUDY; CULTURE MEDIUM; HELA CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; LEGIONELLA PNEUMOPHILA; MICROTUBULE; MODEL; PRIORITY JOURNAL; PROTEIN EXPRESSION;

BACTERIAL ADHESION; CELL FRACTIONATION; COLCHICINE; COLONY COUNT, MICROBIAL; CYTOCHALASIN D; CYTOSKELETON; GALACTOSE; GLUCOSE; HELA CELLS; HUMANS; LEGIONELLA PNEUMOPHILA; MICROSCOPY, ELECTRON; NOCODAZOLE; ORGANELLES; VIRULENCE;

BACTERIA (MICROORGANISMS); LEGIONELLA PNEUMOPHILA; MAMMALIA; NEGIBACTERIA;

EID: 0031819378     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/cjm-44-5-430     Document Type: Article

References (49)

1. Abu Kwaik, Y. 1996. The phagosome containing Legionella pneumophila within the protozoan Hartmanella vermiformis is surrounded by the rough endoplasmic reticulum. Appl. Environ. Microbiol. 62: 2022-2028.
2. Abu Kwaik, Y., Eisenstein, B.I., and Engleberg, N.C. 1993. Phenotypic modulation by Legionella pneumophila upon infection of macrophages. Infect. Immun. 61: 1320-1329.
3. Abu Kwaik, Y., Fields, B.S., and Engleberg, N.C. 1994. Protein expression by the protozoan Hartmanella vermiformis upon contact with its bacterial parasite Legionella pneumophila. Infect. Immun. 62: 1860-1866.
4. Bellinger-Kawahara, C., and Horwitz, M.A. 1990. Complement component C3 fixes selectively to the major outer membrane protein (MOMP) of Legionella pneumophila and mediates phagocytosis of liposome-MOMP complexes by human monocytes. J. Exp. Med. 172: 1201-1210.
5. Birkness, K.A., George, V.G., White, E.H., Stephens, D.S., and Quinn, F.D. 1992. Intracellular growth of Afipia felis, a putative etiologic agent of cat scratch disease. Infect. Immun. 60: 2281-2287.
6. Butler, C.A., and Hoffman, P.S. 1990. Characterization of a major 31-kilodalton peptidoglycan-bound protein of Legionella pneumophila. J. Bacteriol. 172: 2401-2407.
7. Cattani, L., Goldoni, P., Pastoris, M.C., Sinibaldi, L., and Orsi, N. 1997. Bafilomycin A1 and intracellular multiplication of Legionella pneumophila. Antimicrob. Agents Chemother. 41: 212-214.
8. Chandler, F.W., Cole, R.M., Hicklin, M.D., Blackmon, J.A., and Callaway, C.S. 1979. Ultrastructure of the Legionnaires' disease bacterium. Ann. Intern. Med. 90: 642-647.
9. Cirillo, J.D., Falkow, S., and Tompkins, L.S. 1994. Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion. Infect. Immun. 62: 3254-3261.
10. Daisy, J.A., Benson, C.E., McKitrick, J., and Friedman, H.M. 1981. Intracellular replication of Legionella pneumophila. J. Infect. Dis. 143: 460-464.
11. Dreyfus, L.A. 1987. Virulence associated ingestion of Legionella pneumophila. Microb. Pathogen. 3: 45-52.
12. Elliott, J.A., and Winn, W.C. 1986. Treatment of alveolar macrophages with cytochalasin D inhibits uptake and subsequent growth of Legionella pneumophila. Infect. Immun. 51: 31-36.
13. Falkow, S. 1991. Bacterial entry into eukaryotic cells. Cell. 65: 1099-1102.
14. Fernandez, R.C., Lee, S.H.S., Haldane, D., Sumarah, R., and Rozee, K.R. 1989. Plaque assay for virulent Legionella pneumophila. J. Clin. Microbiol. 27: 1961-1964.
15. Fernandez, R.C., Logan, S.M., Lee. S.H.S., and Hoffman. P.S. 1996. Elevated levels of Legionella pneumophila stress protein Hsp60 early in infection of human monocytes and L929 cells correlate with virulence. Infect. Immun. 64: 1968-1976.
16. Fields, B.S. 1993. Legionella and protozoa: interaction of a pathogen and its natural host. In Legionella, current status and emerging perspectives. Edited by J.M. Barbaree, R.F. Breiman, and A.P. Dufour. American Society of Microbiology, Washington, D.C. pp. 129-136.
17. Fields, B.S. 1996. The molecular ecology of legionellae. Trends Microbiol. 4: 286-290.
18. Fields, B.S., Sanden, G.N., Barbaree, J.M., Morrill, W.E., Wadowsky, R.M., White, E.H., and Feeley, J.C. 1989. Intracellular multiplication of Legionella pneumophila in amoebae isolated from hospital hot water tanks. Curr. Microbiol. 18: 131-137.
19. Finlay, B.B., and Falkow, S. 1997. Common themes in microbial pathogenicity revisited. Microbiol. Mol. Biol. Rev. 61: 136-169.
20. Finlay, B.B., Heffron, F., and Falkow, S. 1989. Epithelial cell surfaces induce Salmonella proteins required for bacterial adherence and invasion. Science (Washington, D.C.), 243: 940-943.
21. Gahring, L.C., Heffron, F., Finlay, B.B., and Falkow, S. 1990. Invasion and replication of Salmonella typhimurium in animal cells. Infect. Immun. 58: 443-448.
22. Gibson, F.C., III, Tzianabos, A.O., and Rodgers. F.G. 1994. Adherence of Legionella pneumophila to U-937 cells, guinea pig alveolar macrophages, and MRC-5 cells by a novel, complement-independent binding mechanism. Can. J. Microbiol. 40: 865-872.
23. Hanaichi, T., Sato, T., Iwamoto, T., Malavasi-Yamashiro, Y., Hoshino, M., and Mizuno, N. 1986. A stable stain by modification of Sato's method. J. Electron Microsc. 35: 304-306.
24. Hoffman, P.S. 1997. Invasion of eukaryotic cells by Legionella pneumophila: a common strategy for all hosts? Can. J. Infect. Dis. 8: 139-146.
25. Hoffman, P.S., Butler, C.A., and Quinn, F.D. 1989. Cloning and temperature-dependent expression in Escherichia coli of a Legionella pneumophila gene coding for a genus-common 60-kilodalton antigen. Infect. Immun. 57: 1731-1739.
26. Hoffman, P.S., Houston, L., and Butler, C. A. 1990. Legionella pneumophila htpAB heat shock operon: nucleotide sequence and expression of the 60-kilodalton antigen in L. pneumophila-infected HeLa cells. Infect. Immun. 58: 3380-3387.
27. Horwitz, M.A. 1983. Formation of a novel phagosome by the legionnaires' disease bacterium (Legionella pneumophila) in human monocytes. J. Exp. Med. 158: 1319-1331.
28. Horwitz, M.A. 1984. Phagocytosis of the Legionnaires' disease bacterium (Legionella pneumophila) occurs by a novel mechanism: engulfment within a pseudopod coil. Cell, 36: 27-33.
29. Horwitz, M.A. 1987. Characterization of avirulent mutant Legionella pneumophila that survive but do not multiply within human monocytes. J. Exp. Med. 166: 1310-1328.
30. Horwitz, M.A., and Silverstein, S.C. 1983. Intracellular multiplication of legionnaires' disease bacteria (Legionella pneumophila) in human monocytes is reversibly inhibited by erythromycin and rifampicin. J. Clin. Invest. 71: 15-26.
31. King, C.H., Fields, B.S., Shotts, E.M., Jr., and White, E.H. 1991. Effects of cytochalasin D and methylamine in intracellular growth of Legionella pneumophila in amoebae and human monocyte-like cells. Infect. Immun. 59: 758-763.
32. McDade, J.E. 1979. Primary isolation using guinea pigs and embryonated eggs. In "Legionnaires'," the disease, the bacterium and methodology. HEW Publication No. (CDC)79-8375. Edited by G.L. Jones and G.A. Hébert. U.S. Department of Health, Education, and Welfare, Atlanta, Ga. pp. 69-75.
33. McGee, Z.A , Gorby, G.L., Wyrick, P.B., Hodinka, R., and Hoffman, L.H. 1988. Parasite-directed endocytosis. Rev. Infect. Dis. 10(Suppl.):S311-S316.
34. Michael, E.J., McClellan, M., Huebner, H., and Hostetter, M.K. 1995. Expression of CD21 and synthesis of its ligands by HeLa cells after growth in serum-free medium. J. Lab. Clin. Med. 125: 102-112.
35. Miller, V.L. 1995. Tissue-culture invasion: fact or artefact? Trends Microbiol. 3:69-71.
36. Mintz, C.S., Arnold, P.I., Johnson, W., and Schultz, D.R. 1995. Antibody-independent binding of complement component C1q by Legionella pneumophila. Infect. Immun. 63: 4939-4943.
37. Mody, C.H., Paine, R., III, Shahrabadi, M.S., Simon, R.H., Pearlman, E., Eisenstein, B.I., and Toews, G.B. 1993. Legionella pneumophila replicates within rat alveolar epithelial cells. J. Infect. Dis. 167: 1138-1145.
38. Oldham, L.J., and Rodgers, F.G. 1985. Adhesion, penetration and intracellular replication of Legionella pneumophila: an in vitro model of pathogenesis. J. Gen. Microbiol. 131: 697-706.
39. Pascule, A.W., Feeley, J.C., Gibson, R.J., Cordes, L.G., Meyerowitz, R.L., Patton, C.M., Gorman, G.W., Carmack, C.L., Ezzell, J.W., and Dowling, J.N., 1980. Pittsburg pneumonia agent: direct isolation from human lung tissue. J. Infect. Dis. 141: 727-732.
40. Payne, N.R., and Horwitz, M.A. 1987. Phagocytosis of Legionella pneumophila is mediated by human monocyte complement receptors. J. Exp. Med. 166: 1377-1389.
41. Rodgers, E.G., and Gibson, F.C., III. 1993. Opsonin-independent adherence and intracellular development of Legionella pneumophila within U-937 cells. Can. J. Microbiol. 39: 718-722.
42. Rowbotham, T.J. 1986. Current views on the relationships between amoebae, legionellae and man. Isr. J. Med. Sci. 22: 678-689.
43. Sadosky, A.B., Wiater, L.A., and Shuman, H.A. 1993. Identification of Legionella pneumophila genes required for growth within and killing of human macrophages. Infect. Immun. 61: 5361-5373.
44. Susa, M., Hacker, J., and Marre, R. 1996. De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells. Infect. Immun. 64: 1679-1684.
45. Swanson, M.S., and Isberg, R.R. 1995. Association of Legionella pneumophila with the macrophage endoplasmic reticulum. Infect. Immun. 63: 3609-3620.
46. Tamura, A., Matsumoto, A., Manire, G.P., and Higashi, N. 1971. Electron microscopic observations on the structure of the envelopes of mature elementary bodies and developmental reticulate forms of Chlamydia psittaci. J. Bacteriol. 105: 355-360.
47. Tang, P., Foubister, V., Pucciarelli, M.G., and Finlay, B.B. 1993. Methods to study bacterial invasion. J. Microbiol. Methods. 18: 227-240.
48. Venkataraman, C., Haack, B.J., Bondada, S., and Abu Kwaik, Y. 1997. Identification of a Gal/GalNAc lectin in the protozoan Hartmanella vermiformis as a potential receptor for attachment and invasion by the Legionnaires' disease bacterium. J. Exp. Med. 186: 537-547.
49. Weaver, R.E., and Feeley, J.C. 1979. Cultural and biochemical characterization of the Legionnaires' disease bacterium. In "Legionnaires'," the Disease, the bacterium and methodology. HEW Publication No. (CDC)79-8375. Edited by G.L. Jones and G.A. Hébert. US Department of Health, Education, and Welfare., Atlanta, Ga. pp. 19-25.