High-yield expression in Escherichia coli and purification of mouse ubiquitin-activating enzyme E1

Molecular Biotechnology

Volumn 51, Issue 3, 2012, Pages 254-261

  Carvalho, Andreia F ^a   Pinto, Manuel P ^a   Grou, Cláudia P ^a   Vitorino, Rui ^b   Domingues, Pedro ^b   Yamao, Fumiaki ^c   Sá Miranda, Clara ^a   Azevedo, Jorge E ^a,d  

^a UNIVERSITY OF PORTO   (Portugal)

^b UNIVERSITY OF AVEIRO   (Portugal)

^c NATIONAL INSTITUTE OF GENETICS   (Japan)

^d UNIVERSITY OF PORTO   (Portugal)

Author keywords

Escherichia coli; In vitro ubiquitination assays; Purification; UBA1; Ubiquitin activating enzyme

Indexed keywords

EXPRESSION IN ESCHERICHIA COLI; LARGE AMOUNTS; LOW TEMPERATURES; MAMMALIAN ENZYMES; NATURAL SOURCES; PROTEIN INDUCTION; UBA1; UBIQUITINATION;

AMINO ACIDS; ESCHERICHIA COLI; MAMMALS; PROTEINS; PURIFICATION;

ENZYMES;

HISTIDINE; UBIQUITIN ACTIVATING ENZYME E1; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG; CELL RECEPTOR; DRUG DERIVATIVE; HYBRID PROTEIN; PEROXISOME TARGETING SIGNAL 1 RECEPTOR; PEROXISOME-TARGETING SIGNAL 1 RECEPTOR;

ARTICLE; BACTERIUM CULTURE; CODON; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; LOW TEMPERATURE; MOUSE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN INDUCTION; ANIMAL; BIOSYNTHESIS; CHEMISTRY; CYTOLOGY; ENZYME ASSAY; ENZYMOLOGY; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; LIVER; MASS SPECTROMETRY; METABOLISM; TEMPERATURE;

ESCHERICHIA COLI; MAMMALIA;

ANIMALS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME ASSAYS; ESCHERICHIA COLI; HISTIDINE; LIVER; MICE; RECEPTORS, CYTOPLASMIC AND NUCLEAR; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE; UBIQUITIN-ACTIVATING ENZYMES;

EID: 84871886300     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9463-x     Document Type: Article

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