Expression, purification and characterization of human ubiquitin-activating enzyme, UBE1

Molecular Biology Reports

Volumn 37, Issue 3, 2010, Pages 1413-1419

  Zheng, Mei ^a   Liu, Jing ^a   Yang, Zhenxing ^a   Gu, Xing ^a   Li, Fan ^a   Lou, Tiantian ^a   Ji, Chaoneng ^a   Mao, Yumin ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

High energy thioester bond; Purification; UB; Ubiquitin activating enzyme E1

Indexed keywords

LIGASE; RECOMBINANT ENZYME; SEPHAROSE; THIOESTER; UBIQUITIN; UBIQUITIN PROTEIN LIGASE; UBIQUITIN PROTEIN LIGASE 1; UBIQUITIN PROTEIN LIGASE 2; UNCLASSIFIED DRUG; PRIMER DNA; UBE1 PROTEIN, HUMAN;

ARTICLE; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; HETEROLOGOUS EXPRESSION; IN VITRO STUDY; NONHUMAN; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

DNA PRIMERS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; HUMANS; SEPHAROSE; UBIQUITIN; UBIQUITIN-ACTIVATING ENZYMES;

EID: 77951924048     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9525-3     Document Type: Article

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