Autophagy, senescence, and apoptosis

Methods in Molecular Biology

Volumn 962, Issue , 2013, Pages 31-48

  Goehe, Rachel W ^a   Bristol, Molly L ^a   Wilson, Eden N ^a   Gewirtz, David A ^b  

^a VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

^b VIRGINIA COMMONWEALTH UNIVERSITY   (United States)

Author keywords

Acridine orange; Annexin V; Apoptosis; Autophagy; Flow cytometry; Fluorescent microscopy; Propidium iodide; Senescence; Western immunoblotting; Galactosidase

Indexed keywords

EID: 84871868506     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-236-0_3     Document Type: Article

References (30)

1. Tasdemir E et al (2008) A dual role of p53 in the control of autophagy. Autophagy 4(6):810-814
2. Boya P et al (2005) Inhibition of macroau-tophagy triggers apoptosis. Mol Cell Biol 25(3):1025-1040
3. Klionsky DJ et al (2008) Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes. Autophagy 4(2):151-175
4. Lambert LA et al (2008) Autophagy: a novel mechanism of synergistic cytotoxicity between doxorubicin and roscovitine in a sarcoma model. Cancer Res 68(19):7966-7974
5. Livesey KM et al (2009) Autophagy inhibition in combination cancer treatment. Curr Opin Investig Drugs 10(12):1269-1279
6. Wilson EN, Bristol ML et al (2011) A switch between cytoprotective and cytotoxic autophagy in radiosensitization of breast tumor cells by chloroquine and vitamin D. Horm Cancer 2(5):272-285
7. Mizushima N (2004) Methods for monitoring autophagy. Int J Biochem Cell Biol 36(12): 2491-2502
8. Schmitt CA et al (2002) Dissecting p53 tumor suppressor functions in vivo. Cancer Cell 1(3):289-298
9. Shiloh Y (2003) ATM and related protein kinases: safeguarding genome integrity. Nat Rev Cancer 3(3):155-168
10. Taylor RC, Cullen SP, Martin SJ (2008) Apoptosis: controlled demolition at the cellular level. Nat Rev Mol Cell Biol 9(3):231-241
11. Campisi J, d'Adda di Fagagna F (2007) Cellular senescence: when bad things happen to good cells. Nat Rev Mol Cell Biol 8(9):729-740
12. Chen Z et al (2005) Crucial role of p53-depen-dent cellular senescence in suppression of Pten-deficient tumorigenesis. Nature 436(7051):725-730
13. Sharpless NE, DePinho RA (2005) Cancer: crime and punishment. Nature 436(7051): 636-637
14. Widmann C, Gibson S, Johnson GL (1998) Caspase-dependent cleavage of signaling proteins during apoptosis. A turn-off mechanism for anti-apoptotic signals. J Biol Chem 273(12):7141-7147
15. Schwartzman RA, Cidlowski JA (1993) Apoptosis: the biochemistry and molecular biology of programmed cell death. Endocr Rev 14(2):133-151
16. Boe R et al (1991) The protein phosphatase inhibitor okadaic acid induces morphological changes typical of apoptosis in mammalian cells. Exp Cell Res 195(1):237-246
17. Shen Y, White E (2001) p53-dependent apop-tosis pathways. Adv Cancer Res 82:55-84
18. Haupt S et al (2003) Apoptosis - the p53 network. J Cell Sci 116(Pt 20):4077-4085
19. Paglin S et al (2001) A novel response of cancer cells to radiation involves autophagy and formation of acidic vesicles. Cancer Res 61(2):439-444
20. Traganos F, Darzynkiewicz Z (1994) L ysosomal proton pump activity: supravital cell staining with acridine orange differentiates leukocyte subpopulations. Methods Cell Biol 41:185-194
21. Pankiv S et al (2007) p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy. J Biol Chem 282(33):24131-24145
22. Lowry OH et al (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193(1):265-275
23. Ben-Porath I, Weinberg RA (2004) When cells get stressed: an integrative view of cellular senescence. J Clin Invest 113(1):8-13
24. Campisi J (2005) Senescent cells, tumor suppression, and organismal aging: good citizens, bad neighbors. Cell 120(4):513-522
25. Kurz DJ et al (2000) Senescence-associated (beta)-galactosidase re flects an increase in lyso-somal mass during replicative ageing of human endothelial cells. J Cell Sci 113(Pt 20): 3613-3622
26. Amaravadi RK, Thompson CB (2007) The roles of therapy-induced autophagy and necrosis in cancer treatment. Clin Cancer Res 13(24):7271-7279
27. Boya P et al (2003) Mitochondrial membrane permeabilization is a critical step of lysosome-initiated apoptosis induced by hydroxychloro-quine. Oncogene 22(25):3927-3936
28. Tasdemir E et al (2008) Methods for assessing autophagy and autophagic cell death. Methods Mol Biol 445:29-76
29. Yoshimori T et al (1991) Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells. J Biol Chem 266(26):17707-17712
30. Vermes I et al (1995) A novel assay for apopto-sis. Flow cytometric detection of phosphatidyl-serine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184(1):39-51