Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a diagnostic tool of Toxoplasma gondii in pork

Veterinary Parasitology

Volumn 192, Issue 1-3, 2013, Pages 98-103

  Qu, Daofeng ^a   Zhou, Huaiyu ^b   Han, Jianzhong ^a   Tao, Siyue ^a   Zheng, Bailing ^c   Chi, Na ^a   Su, Chunlei ^d   Du, Aifang ^e  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

^b SHANDONG UNIVERSITY   (China)

^c Xiuzhou Animal Husbandry and Veterinary Bureau   (China)

^d UNIVERSITY OF TENNESSEE   (United States)

^e ZHEJIANG UNIVERSITY   (China)

Author keywords

Detection; Pork; RT LAMP; T. gondii

Indexed keywords

RNA 18S; PRIMER DNA; PROTOZOAL DNA; PROTOZOAL RNA;

ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FOOD CONTAMINATION; FOOD CONTROL; FOOD QUALITY; FOOD SAFETY; INTERMETHOD COMPARISON; LIMIT OF DETECTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PORK; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TACHYZOITE; TOXOPLASMA GONDII; AGAR GEL ELECTROPHORESIS; ANIMAL; GENETICS; ISOLATION AND PURIFICATION; MEAT; NUCLEIC ACID AMPLIFICATION; PARASITOLOGY; SPECIES DIFFERENCE; SWINE; SWINE DISEASE; TOXOPLASMA; TOXOPLASMOSIS; VETERINARY;

TOXOPLASMA GONDII;

ANIMALS; DNA PRIMERS; DNA, PROTOZOAN; ELECTROPHORESIS, AGAR GEL; FOOD PARASITOLOGY; MEAT; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; SWINE; SWINE DISEASES; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL;

EID: 84871700782     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2012.10.010     Document Type: Article

References (24)

1. Alhassan A., Thekisoe O.M., Yokoyama N., Inoue N., Motloang M.Y., Mbati P.A., Yin H., Katayama Y., Anzai T., Sugimoto C., Igarashi I. Development of loop-mediated isothermal amplification (LAMP) method for diagnosis of equine piroplasmosis. Vet. Parasitol. 2007, 143:155-160.
2. Barbosa B.F., Gomes A.O., Ferro E.A., Napolitano D.R., Mineo J.R., Silva N.M. Enrofloxacin is able to control Toxoplasma gondii infection in both in vitro and in vivo experimental models. Vet. Parasitol. 2012, 187:44-52.
3. Barkway C.P., Pocock R.L., Vrba V., Blake D.P. Loop-mediated isothermal amplification (LAMP) assays for the species-specific detection of Eimeria that infect chickens. BMC Vet. Res. 2011, 7:67.
4. Buates S., Bantuchai S., Sattabongkot J., Han E.T., Tsuboi T., Udomsangpetch R., Sirichaisinthop J., Tan-ariya P. Development of a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) for clinical detection of Plasmodium falciparum gametocytes. Parasitol. Int. 2010, 59:414-420.
5. Cardoso T.C., Ferrari H.F., Bregano L.C., Silva-Frade C., Rosa A.C., Andrade A.L. Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye. Mol. Cell. Probes 2010, 24:415-417.
6. El-Nawawi F.A., Tawfik M.A., Shaapan R.M. Methods for inactivation of Toxoplasma gondii cysts in meat and tissues of experimentally infected sheep. Foodborne Pathog. Dis. 2008, 5:687-690.
7. Furtado J.M., Smith J.R., Belfort R., Gattey D., Winthrop K.L. Toxoplasmosis: a global threat. J. Glob. Infect. Dis. 2011, 3:281-284.
8. He L., Xu H.S., Wang M.Z., Rong H.N. Development of rapid detection of infectious hypodermal and hematopoietic necrosis virus by loop-mediated isothermal amplification. Bing Du Xue Bao 2010, 26:490-495.
9. Hobo S., Niwa H., Oku K. Development and application of loop-mediated isothermal amplification methods targeting the seM gene for detection of Streptococcus equi Subsp. equi. J. Vet. Med. Sci. 2011.
10. Innes E.A., Bartley P.M., Rocchi M., Benavidas-Silvan J., Burrells A., Hotchkiss E., Chianini F., Canton G., Katzer F. Developing vaccines to control protozoan parasites in ruminants: dead or alive?. Vet. Parasitol. 2011, 180:155-163.
11. Iseki H., Alhassan A., Ohta N., Thekisoe O.M., Yokoyama N., Inoue N., Nambota A., Yasuda J., Igarashi I. Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites. J. Microbiol. Methods 2007, 71:281-287.
12. Karanis P., Ongerth J. LAMP-a powerful and flexible tool for monitoring microbial pathogens. Trends Parasitol. 2009, 25:498-499.
13. Lee M.S., Lin Y.C., Lai G.H., Lai S.Y., Chen H.J., Wang M.Y. One-step reverse-transcription loop-mediated isothermal amplification for detection of infectious bursal disease virus. Can. J. Vet. Res. 2011, 75:122-127.
14. Lunden A., Uggla A. Infectivity of Toxoplasma gondii in mutton following curing, smoking, freezing or microwave cooking. Int. J. Food Microbiol. 1992, 15:357-363.
15. Nagamine K., Hase T., Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probes 2002, 16:223-229.
16. Nagamine K., Kuzuhara Y., Notomi T. Isolation of single-stranded DNA from loop-mediated isothermal amplification products. Biochem. Biophys. Res. Commun. 2002, 290:1195-1198.
17. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000, 28:E63.
18. Qu D.F., Yu H.J., Liu Z., Zhang D.F., Zhou Q.J., Zhang H.L., Du A.F. Ginsenoside Rg1 enhances immune response induced by recombinant Toxoplasma gondii SAG1 antigen. Vet. Parasitol. 2011, 179:28-34.
19. Sotiriadou I., Karanis P. Evaluation of loop-mediated isothermal amplification for detection of Toxoplasma gondii in water samples and comparative findings by polymerase chain reaction and immunofluorescence test (IFT). Diagn. Microbiol. Infect. Dis. 2008, 62:357-365.
20. Thekisoe O.M., Inoue N., Kuboki N., Tuntasuvan D., Bunnoy W., Borisutsuwan S., Igarashi I., Sugimoto C. Evaluation of loop-mediated isothermal amplification (LAMP) PCR and parasitological tests for detection of Trypanosoma evansi in experimentally infected pigs. Vet. Parasitol. 2005, 130:327-330.
21. Varga A., James D. Use of reverse transcription loop-mediated isothermal amplification for the detection of Plum pox virus. J. Virol. Methods 2006, 138:184-190.
22. Zhang H., Thekisoe O.M., Aboge G.O., Kyan H., Yamagishi J., Inoue N., Nishikawa Y., Zakimi S., Xuan X. Toxoplasma gondii: sensitive and rapid detection of infection by loop-mediated isothermal amplification (LAMP) method. Exp. Parasitol. 2009, 122:47-50.
23. Zhao K., Liu Y., Wang X. Reverse transcription loop-mediated isothermal amplification of DNA for detection of Barley yellow dwarf viruses in China. J. Virol. Methods 2010, 169:211-214.
24. Zintl A., Halova D., Mulcahy G., O'Donovan J., Markey B., DeWaal T. In vitro culture combined with quantitative TaqMan PCR for the assessment of Toxoplasma gondii tissue cyst viability. Vet. Parasitol. 2009, 164:167-172.