Visual detection of turkey coronavirus RNA in tissues and feces by reverse-transcription loop-mediated isothermal amplification (RT-LAMP) with hydroxynaphthol blue dye

Molecular and Cellular Probes

Volumn 24, Issue 6, 2010, Pages 415-417

  Cardoso, Tereza C ^a   Ferrari, Heitor F ^a   Bregano, Lívia C ^a   Silva Frade, Camila ^a   Rosa, Ana Carolina G ^a   Andrade, Alexandre L ^a  

^a SÃO PAULO STATE UNIVERSITY UNESP   (Brazil)

Author keywords

Hydroxynaphthol blue; LAMP assay; Spike gene; Turkey coronavirus

Indexed keywords

DYE; HYDROXYNAPHTHOL BLUE; UNCLASSIFIED DRUG; VIRUS RNA;

ANIMAL TISSUE; ARTICLE; CECUM; CORONAVIRUS; CROSS REACTION; EMBRYO; FECES ANALYSIS; ILEUM; INTERMETHOD COMPARISON; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION LOOP MEDIATED ISOTHERMAL AMPLIFICATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; VIRUS GENOME;

ANIMALS; COLORING AGENTS; CORONAVIRUS, TURKEY; FECES; NAPHTHALENESULFONATES; ORGAN SPECIFICITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; TEMPERATURE; TURKEYS;

AVES; TURKEY CORONAVIRUS;

EID: 78049287612     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2010.08.003     Document Type: Article

References (14)

1. Teixeira M.C., Luvizotto M.C., Ferrari H.F., Mendes A.R., da Silva S.E., Cardoso T.C. Detection of turkey coronavirus in commercial turkey poults in Brazil. Avian Pathol 2007, 36(1):29-33.
2. Culver F., Dziva F., Cavanagh D., Stevens M.P. Poult enteritis and mortality syndrome in turkeys in Great Britain. Vet Rec 2006, 159(7):209-210.
3. Pantin-Jackwood M.J., Day J.M., Jackwood M.W., Spackman E. Enteric viruses detected by molecular methods in commercial chicken and turkey flocks in the United States between 2005 and 2006. Avian Dis 2008, 52(2):235-244.
4. Chen Y.-N., Wu C.C., Bryan T., Hooper T., Schrader D., Lin T.L. Specific real-time reverse transcription polymerase chain reaction for detection and quantification of turkey coronavirus RNA in tissues and feces from turkeys infected with turkey coronavirus. J Virol Methods 2010, 163:452-458.
5. Cavanagh D. Coronaviruses in poultry and other birds. Avian Pathol 2005, 34(6):439-448.
6. Masters P. The molecular biology of coronavirus. Adv Virus Res 2006, 66:193-292.
7. Guy J.S. Isolation and propagation of coronaviruses in embryonated eggs. Methods Mol Biol 2008, 454:109-117.
8. Cardoso T.C., Castanheira T.L., Teixeira M.C.B., Rosa A.C.G., Hirata K.Y., Astolphi R.D., et al. Validation of an immunohistochemistry assay to detect turkey coronavirus: a rapid and simple screening tool for limited resource settings. Poult Sci 2008, 87(7):1347-1352.
9. Gomes D.E., Hirata K.S., Saheki K., Rosa A.C.G., Luvizotto M.C.R., Cardoso T.C. Pathology and virus tissue distribution of turkey coronavirus (TCoV) in experimentally infected chicks and turkey poults. J Comp Pathol 2010, 147:8-13.
10. Jackwood M.W., Boynton T.O., Hilt D.A., McKinley E.T., Kissinger J.C., Paterson A.H., et al. Emergence of a group 3 coronavirus through recombination. Virology 2010, 398(1):98-108.
11. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000, 28:e63.
12. Chen H.T., Zang J., Ma Y.P., Ma L.N., Ding Y.Z., Liu X.T., et al. Reverse transcription loop-mediated isothermal amplification for rapid detection of infectious bronchitis virus in infected chicken tissues. Mol Cell Probes 2010, 24:104-106.
13. Xie Q.-M., Ji J., Pickens T.T., Du L.-Q., Cao Y.-C., Li H.-M., et al. Rapid detection of infectious laryngotracheitis virus isolates by loop-mediated isothermal amplification. J Virol Methods 2010, 165:71-75.
14. Pham H.M., Nakajima C., Ohashi K., Onuma M. Loop-mediated isothermal amplification for rapid detection of Newcastle disease virus. J Clin Microbiol 2005, 43:1646-1650.