Procalcitonin Predicts Real-Time PCR Results in Blood Samples from Patients with Suspected Sepsis

PLoS ONE

Volumn 7, Issue 12, 2012, Pages

  Mencacci, Antonella ^a   Leli, Christian ^a   Cardaccia, Angela ^a   Meucci, Marta ^a   Moretti, Amedeo ^a   D'Alò, Francesco ^a   Farinelli, Senia ^a   Pagliochini, Rita ^a   Barcaccia, Mariella ^a   Bistoni, Francesco ^a  

^a UNIVERSITY OF PERUGIA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

PROCALCITONIN;

AGED; AREA UNDER THE CURVE; ARTICLE; COMPARATIVE EFFECTIVENESS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; DISEASE MARKER; FEMALE; FEVER; HORMONE BLOOD LEVEL; HUMAN; INFECTION RATE; MAJOR CLINICAL STUDY; MALE; NONHUMAN; PREDICTION; PREDICTIVE VALUE; PROTEIN DETERMINATION; REAL TIME POLYMERASE CHAIN REACTION; RECEIVER OPERATING CHARACTERISTIC; SENSITIVITY AND SPECIFICITY; SEPSIS;

AGED; AGED, 80 AND OVER; BACTEREMIA; BIOLOGICAL MARKERS; CALCITONIN; EARLY DIAGNOSIS; FEMALE; FEVER; HUMANS; MALE; MIDDLE AGED; PROTEIN PRECURSORS; REAL-TIME POLYMERASE CHAIN REACTION; SEPSIS;

BACTERIA (MICROORGANISMS);

EID: 84871657867     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0053279     Document Type: Article

References (35)

1. American College of Chest Physicians/Society of Critical Care Medicine Consensus Conference: definitions for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis (1992) Crit Care Med. 20: 864-74.
2. Vincent JL, (2008) Clinical sepsis and septic shock-definition, diagnosis and management principles. Langenbecks Arch Surg 393: 817-24.
3. Emonet S, Schrenzel J, (2011) How could rapid bacterial identification improve the management of septic patients? Expert Rev Anti Infect Ther 9: 707-9.
4. Kumar A, Roberts D, Wood KE, Light B, Parrillo JE, et al. (2006) Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34: 1589-96.
5. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM, (2004) New developments in the diagnosis of bloodstream infections. Lancet Infect Dis 4: 751-60.
6. Lehmann LE, Hunfeld KP, Emrich T, Haberhausen G, Wissing H, et al. (2008) A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples. Med Microbiol Immunol 197: 313-24.
7. Wallet F, Nseir S, Baumann L, Herwegh S, Sendid B, et al. (2010) Preliminary clinical study using a multiplex real-time PCR test for the detection of bacterial and fungal DNA directly in blood. Clin Microbiol Infect 16: 774-9.
8. Dierkes C, Ehrenstein B, Siebig S, Linde HJ, Reischl U, et al. (2009) Clinical impact of a commercially available multiplex PCR system for rapid detection of pathogens in patients with presumed sepsis. BMC Infect Dis 9: 126.
9. Bravo D, Blanquer J, Tormo M, Aguilar G, Borrás R, et al. (2011) Diagnostic accuracy and potential clinical value of the LightCycler SeptiFast assay in the management of bloodstream infections occurring in neutropenic and critically ill patients. Int J Infect Dis 15: 326-31.
10. Pasqualini L, Mencacci A, Leli C, Montagna P, Cardaccia A, et al. (2012) Diagnostic performance of a multiple real-time PCR assay in patients with suspected sepsis hospitalized in an internal medicine ward. J Clin Microbiol 50: 1285-8.
11. Becker KL, Nylén ES, White JC, Müller B, Snider RH Jr, (2004) Clinical review 167: Procalcitonin and the calcitonin gene family of peptides in inflammation, infection, and sepsis: a journey from calcitonin back to its precursors. J Clin Endocrinol Metab 89: 1512-25.
12. Christ-Crain M, Müller B, (2005) Procalcitonin in bacterial infections-hype, hope, more or less? Swiss Med Wkly 135: 451-60.
13. Müller B, Becker KL, (2001) Procalcitonin: how a hormone became a marker and mediator of sepsis. Swiss Med Wkly 131: 595-602.
14. Riedel S, (2012) Procalcitonin and the role of biomarkers in the diagnosis and management of sepsis. Diagn Microbiol Infect Dis 73: 221-7.
15. Schuetz P, Albrich W, Mueller B, (2011) Procalcitonin for diagnosis of infection and guide to antibiotic decisions: past, present and future. BMC Med 9: 107.
16. Tromp M, Lansdorp B, Bleeker-Rovers CP, Gunnewiek JM, Kullberg BJ, et al. (2012) Serial and panel analyses of biomarkers do not improve the prediction of bacteremia compared to one procalcitonin measurement. J Infect 65: 292-301.
17. Kim MH, Lim G, Kang SY, Lee WI, Suh JT, et al. (2011) Utility of procalcitonin as an early diagnostic marker of bacteremia in patients with acute fever. Yonsei Med J 52: 276-81.
18. Weinstein MP, (2003) Blood culture contamination: persisting problems and partial progress. J Clin Microbiol 41: 2275-8.
19. Garner JS, Jarvis WR, Emori TG, Horan TC, Hughes JM, (1988) CDC definitions for nosocomial infections, 1988. Am J Infect Control 16: 128-140.
20. Dellinger RP, Levy MM, Carlet JM, Bion J, Parker MM, et al. (2008) Surviving Sepsis Campaign: international guidelines for management of severe sepsis and septic shock. Crit Care Med 36: 296-327.
21. Andrade SS, Bispo PJ, Gales AC, (2008) Advances in the microbiological diagnosis of sepsis. Shock 30: 41-6.
22. Weile J, Knabbe C, (2009) Current applications and future trends of molecular diagnostics in clinical bacteriology. Anal Bioanal Chem 394: 731-42.
23. Obara H, Aikawa N, Hasegawa N, Hori S, Ikeda Y, et al. (2011) The role of a real-time PCR technology for rapid detection and identification of bacterial and fungal pathogens in whole-blood samples. J Infect Chemother 17: 327-33.
24. Mancini N, Carletti S, Ghidoli N, Cichero P, Burioni R, et al. (2010) The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin Microbiol Rev 23: 235-51.
25. Brenner H, Gefeller O, (1997) Variation of sensitivity, specificity, likelihood ratios and predictive values with disease prevalence. Stat Med 16: 981-91.
26. Müller F, Christ-Crain M, Bregenzer T, Krause M, Zimmerli W, et al. (2010) Procalcitonin levels predict bacteremia in patients with community-acquired pneumonia: a prospective cohort trial. Chest 138: 121-9.
27. Mauro MV, Cavalcanti P, Perugini D, Noto A, Sperlì D, et al. (2012) Diagnostic utility of LightCycler SeptiFast and procalcitonin assays in the diagnosis of bloodstream infection in immunocompromised patients. Diagn Microbiol Infect Dis 73: 308-11.
28. Sakr Y, Sponholz C, Tuche F, Brunkhorst F, Reinhart K, (2008) The role of procalcitonin in febrile neutropenic patients: review of the literature. Infection 36: 396-407.
29. Schuetz P, Chiappa V, Briel M, Greenwald JL, (2011) Procalcitonin Algorithms for Antibiotic Therapy Decisions. Archives of Internal Medicine 171: 1322-31.
30. Knudsen JB, Fuursted K, Petersen E, Wierup P, Mølgaard H, et al. (2010) Procalcitonin in 759 patients clinically suspected of infective endocarditis. Am J Med 123: 1121-7.
31. Mencacci A, Leli C, Montagna P, Cardaccia A, Meucci M, et al. (2012) Diagnosis of infective endocarditis: comparison of the LightCycler SeptiFast real-time PCR with blood culture. J Med Microbiol 61: 881-3.
32. Schuetz P, Mueller B, Trampuz A, (2007) Serum procalcitonin for discrimination of blood contamination from bloodstream infection due to coagulase-negative staphylococci. Infection 35: 352-5.
33. Plorde JJ, Tenover FC, Carlson LG, (1985) Specimen volume versus yield in the BACTEC blood culture system. J Clin Microbiol 22: 292-5.
34. Schuetz P, Christ-Crain M, Huber AR, Müller B, (2010) Long-term stability of procalcitonin in frozen samples and comparison of Kryptor and VIDAS automated immunoassays. Clin Biochem 343: 341-4.
35. Hausfater P, Brochet C, Freund Y, Charles V, Bernard M, (2010) Procalcitonin measurement in routine emergency medicine practice: Comparison between two immunoassays. Clin Chem Lab Med 48: 501-4.