MLVA-16 loci panel on Brucella spp. using multiplex PCR and multicolor capillary electrophoresis

Journal of Microbiological Methods

Volumn 92, Issue 2, 2013, Pages 103-107

  Garofolo, Giuliano ^a   Ancora, Massimo ^a   Di Giannatale, Elisabetta ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

Author keywords

Brucella spp.; Capillary electrophoresis; Genotyping; MLVA

Indexed keywords

ALLELE; ARTICLE; BACTERIAL STRAIN; BRUCELLA; CAPILLARY ELECTROPHORESIS; GENE LOCUS; GENETIC ANALYSIS; GENOTYPE; MULTICOLOR CAPILLARY ELECTROPHORESIS; MULTILOCUS VARIABLE NUMBER TANDEM REPEAT ANALYSIS 16 LOCI PANEL; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PRIORITY JOURNAL; VARIABLE NUMBER OF TANDEM REPEAT;

BRUCELLA; ELECTROPHORESIS, CAPILLARY; HUMANS; MINISATELLITE REPEATS; MOLECULAR TYPING; MULTIPLEX POLYMERASE CHAIN REACTION;

BRUCELLA;

EID: 84871517458     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.11.007     Document Type: Article

References (12)

1. Al Dahouk S., Fleche P.L., Nockler K., Jacques I., Grayon M., Scholz H.C., Tomaso H., Vergnaud G., Neubauer H. Evaluation of Brucella MLVA typing for human brucellosis. J. Microbiol. Methods 2007, 69:137-145.
2. Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res. 1999, 27:573-580.
3. Bricker B.J., Ewalt D.R., Halling S.M. Brucella "HOOF-Prints": strain typing by multi-locus analysis of variable number tandem repeats (VNTRs). BMC Microbiol. 2003, 3:15.
4. Corbel M.J. Brucellosis: an overview. Emerg. Infect. Dis. 1997, 3:213-221.
5. De Santis R., Ciammaruconi A., Faggioni G., Fillo S., Gentile B., Di Giannatale E., Ancora M., Lista F. High throughput MLVA-16 typing for Brucella based on the microfluidics technology. BMC Microbiol. 2011, 11:60.
6. Hoyer B.H., McCullough N.B. Homologies of deoxyribonucleic acids from Brucella ovis, canine abortion organisms, and other Brucella species. J. Bacteriol. 1968, 96:1783-1790.
7. Pappas G. The changing Brucella ecology: novel reservoirs, new threats. Int. J. Antimicrob. Agents 2010, 36(Suppl. 1):S8-S11.
8. Untergasser A., Nijveen H., Rao X., Bisseling T., Geurts R., Leunissen J.A. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res. 2007, 35:W71-W74.
9. van Belkum A. Tracing isolates of bacterial species by multilocus variable number of tandem repeat analysis (MLVA). FEMS Immunol. Med. Microbiol. 2007, 49:22-27.
10. Whatmore A.M. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. Infect. Genet. Evol. 2009, 9:1168-1184.
11. Whatmore A.M., Shankster S.J., Perrett L.L., Murphy T.J., Brew S.D., Thirlwall R.E., Cutler S.J., MacMillan A.P. Identification and characterization of variable-number tandem-repeat markers for typing of Brucella spp. J. Clin. Microbiol. 2006, 44:1982-1993.
12. Yokoyama E., Kishida K., Uchimura M., Ichinohe S. Comparison between agarose gel electrophoresis and capillary electrophoresis for variable numbers of tandem repeat typing of Mycobacterium tuberculosis. J. Microbiol. Methods 2006, 65:425-431.