High throughput MLVA-16 typing for Brucella based on the microfluidics technology

BMC Microbiology

Volumn 11, Issue , 2011, Pages

  De Santis, Riccardo ^a   Ciammaruconi, Andrea ^a   Faggioni, Giovanni ^a   Fillo, Silvia ^a   Gentile, Bernardina ^a   Di Giannatale, Elisabetta ^b   Ancora, Massimo ^b   Lista, Florigio ^a  

^a Army Medical and Veterinary Research Center   (Italy)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL ABRUZZO E DEL MOLISE G CAPORALE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BRUCELLA; BRUCELLA ABORTUS; BRUCELLA MELITENSIS; BRUCELLA SUIS; DNA SEQUENCE; GENE AMPLIFICATION; GENOTYPE; MICROFLUIDICS; MLVA 16 TYPING; NONHUMAN; VALIDATION PROCESS; BRUCELLOSIS; CLASSIFICATION; EVALUATION; GENETICS; HIGH THROUGHPUT SCREENING; METHODOLOGY; MICROBIOLOGY; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING;

BRUCELLA;

BACTERIAL TYPING TECHNIQUES; BRUCELLA; BRUCELLOSIS; GENOTYPE; HIGH-THROUGHPUT SCREENING ASSAYS; MICROFLUIDICS; MOLECULAR EPIDEMIOLOGY; MOLECULAR TYPING;

EID: 79953080232     PISSN: 14712180     EISSN: 14712180     Source Type: Journal    
DOI: 10.1186/1471-2180-11-60     Document Type: Article

References (35)

1. The new global map of human brucellosis. G Pappas P Papadimitriou N Akritidis L Christou EV Tsianos, Lancet Infect Dis 2006 6 91 99 10.1016/S1473-3099(06)70382-6 16439329 (Pubitemid 43138907)
2. Human brucellosis: A classical infectious disease with persistent diagnostic challenges. GF Araj, Clin Lab Sci 1999 12 4 207 12 10558306
3. List of Prokaryotic names with Standing in Nomenclature - Genus Brucella. JP Euzeby, 2010 http://www.bacterio.cict.fr/b/brucella.html
4. Current understanding of the genetic diversity of Brucella, an expanding genus of zoonotic pathogens. AM Whatmore, Infect Genet Evol 2009 9 6 1168 84 10.1016/j.meegid.2009.07.001 19628055
5. Brucella microti sp. nov., isolated from the common vole Microtus arvalis. Int J Syst Evol Microbiol. HC Scholz Z Hubalek I Sedlaek G Vergnaud H Tomaso S Al Dahouk F Melzer P Kampfer H Neubauer A Cloeckaert M Maquart MS Zygmunt AM Whatmore E Falsen P Bahn C Göllner MB Pfeffer B Huber H Busse K Nöckler, 2008 58 375 382 18218934
6. Real-time PCR assays of single-nucleotide polymorphisms defining the major Brucella clades. JT Foster RT Okinaka R Svensson K Shaw BK De RA Robison WS Probert LJ Kenefic WD Brown P Keim, Journal of Clinical Microbiology 2008 46 1 296 301 10.1128/JCM.01496-07 18032628
7. Brucella ceti sp. nov. and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts. G Foster BS Osterman J Godfroid I Jacques A Cloeckaert, Int J Syst Evol Microbiol 2007 57 2688 2693 10.1099/ijs.0.65269-0 17978241 (Pubitemid 350187441)
8. Brucella inopinata sp. nov., isolated from a breast implant infection. HC Scholz K Nöckler C Göllner P Bahn G Vergnaud H Tomaso S Al-Dahouk P Kämpfer A Cloeckaert M Maquart MS Zygmunt AM Whatmore M Pfeffer B Huber HJ Busse BK De, Int J Syst Evol Microbiol 2010 60 Pt 4 801 8 10.1099/ijs.0.011148-0 19661515
9. Techniques for the brucellosis. GG Alton LM Jones RD Angus JM Verger, laboratory INRA 1988 ISBN:2-7380-0042-8
10. Typing of Brucella field strains isolated from livestock populations in Italy between 2001 and 2006. E Di Giannatale F De Massis M Ancora K Zilli A Alessiani, Veterinaria Italiana 2008 44 2 383 388 20405440
11. Brucellosis. ET Thorne, In Infectious Diseases of Wild Mammals Manson Publishing, Williams ES, Barker IK, Third 2001 372 395 full-text
12. Evaluation of Brucella MLVA typing for human brucellosis. S Al Dahouk P Le Fleche K Nockler I Jacques M Grayon HC Scholz H Tomaso G Vergnaud H Neubauer, J Microbiol Methods 2007 69 137 145 10.1016/j.mimet.2006.12.015 17261338 (Pubitemid 46437272)
13. Characterization of the genetic diversity of Brucella by multilocus sequencing. AM Whatmore LL Perrett AP MacMillan, BMC Microbiol 2007 7 34 10.1186/1471-2180-7-34 17448232
14. Brucella as a biological weapon. G Pappas P Panagopoulou L Christou N Akritidis, Cell Mol Life Sci 2006 63 2229 36 10.1007/s00018-006-6311-4 16964579 (Pubitemid 44653036)
15. Brucella, a monospecific genus as shown by deoxyribonucleic acid hybridization. JM Verger F Grimont PAD Grimont M Grayon, Int J Syst Bacteriol 1985 35292 295
16. Identification and characterization of Variable-Number Tandem-Repeat Markers for typing of Brucella spp. AM Whatmore SJ Shankster LL Perrett TJ Murphy SD Brew RE Thirlwall SJ Cutler AP MacMillan, J Clin Microbiol 2006 44 1982 1993 10.1128/JCM.02039-05 16757588 (Pubitemid 43902143)
17. Evaluation of a Multilocus Variable-Number Tandem-Repeat Analysis Scheme for Typing Human Brucella Isolates in a Region of Brucellosis Endemicity. MM Kattar RF Jaafar GF Araj P Le Flèche MG Matar RA Rached S Khalife G Vergnaud, J Clin Microbiol 2008 45 3935 3940 10.1128/JCM.00464-08
18. Use of 16 S rRNA gene sequencing for rapid confirmatory identification of Brucella isolates. JE Gee BK De PN Levett AM Whitney RT Novak T Popovic, J Clin Microbiol 2004 42 3649 3654 10.1128/JCM.42.8.3649-3654.2004 15297511 (Pubitemid 39045694)
19. PCR as a diagnostic tool for brucellosis. BJ Bricker, Vet Microbiol 2002 90 435 446 10.1016/S0378-1135(02)00228-6 12414163 (Pubitemid 35279895)
20. Differentiation of Brucella abortus bv. 1, 2 and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. BJ Bricker SM Halling, J Clin Microbiol 1994 32 2660 2666 7852552 (Pubitemid 24319098)
21. Repetitive element sequence based polymerase chain reaction for typing of Brucella strains. E Tscherneva N Rijpens C Naydensky LMF Herman, Vet Microbiol 1996 51 169 178 10.1016/0378-1135(96)00036-3 8828133 (Pubitemid 26303026)
22. Differentiation of Brucella species by random amplified polymorphic DNA analysis. E Tscherneva N Rijpens B Jersek LMF Herman, J Appl Microbiol 2000 88 69 80 10.1046/j.1365-2672.2000.00945.x 10735245 (Pubitemid 30140184)
23. The use of an arbitrarily primed PCR product for the specific detection of Brucella. S AlMomin M Saleem Q Al-Mutawa, World Journal of Microbiology & Biotechnology 1999 15 381 385 (Pubitemid 29463975)
24. Use of amplified fragment length polymorphism to identify and type Brucella isolates of medical and veterinary interest. AM Whatmore TJ Murphy S Shankster E Young S Cutler AP Macmillan, J Clin Microbiol 2005 43 761 769 10.1128/JCM.43.2.761-769.2005 15695677 (Pubitemid 40256032)
25. Molecular characterization of the rpoB gene in Brucella species: new potential molecular markers for genotyping. C Marianelli F Ciuchini M Tarantino P Pasquali R Adone, Microbes Infect 2006 8 3 860 865 10.1016/j.micinf.2005.10. 008 16483820
26. Multiplex Assay based on single-nucleotide polymorphisms for rapid identification of Brucella isolates at the species level. JC Scott MS Koylass MR Stubberfield AM Whatmore, Appl Environ Microbiol 2007 73 22 7331 7337 10.1128/AEM.00976-07 17890328 (Pubitemid 350196684)
27. Identification of Brucella species and biotypes using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). S Al Dahouk H Tomaso E Prenger-Berninghoff WD Splettstoesser HC Scholz H Neubauer, Crit Rev Microbiol 2005 31 4 191 196 10.1080/10408410500304041 16417200 (Pubitemid 41770018)
28. Brucella 'Hoof-Prints': strain typing by multi-locus analysis of variable number tandem repeats (VNTRs). BJ Bricker DR Ewalt SM Halling, BMC Microbiol 2003 3 15 10.1186/1471-2180-3-15 12857351
29. Evaluation and selection of tandem repeat loci for a Brucella MLVA typing assay. P Le Flèche I Jacques M Grayon A Al-Dahouk P Bouchon F Denoeud K Nöckler H Neubauer LA Guilloteau G Vergnaud, BMC Microbiol 2006 143 2913 2921
30. Multiple locus VNTR (variable number of tandem repeat) analysis (MLVA). G Vergnaud C Pourcel, Molecular identification, systematics and population structure of prokaryotes Springer-Verlag, Berlin, Germany, Stackebrandt E, 2006 83 104 full-text
31. Fieldable genotyping of Bacillus anthracis and Yersinia pestis based on 25-loci Multi Locus VNTR Analysis. A Ciammaruconi S Grassi R De Santis G Faggioni V Pittiglio R D'Amelio A Carattoli A Cassone G Vergnaud F Lista, BMC Microbiol 2008 8 21 10.1186/1471-2180-8-21 18230125
32. Lab on a chip genotyping for Brucella spp. based on 15-loci multi locus VNTR analysis. R De Santis A Ciammaruconi G Faggioni R D'Amelio C Marianelli F Lista, BMC Microbiol 2009 9 66 10.1186/1471-2180-9-66 19351390
33. Molecular epidemiological and antibiotic susceptibility characterization of Brucella isolates from humans in Sicily, Italy. C Marianelli C Graziani C Santangelo MT Xibilia A Imbriani R Amato D Neri M Cuccia S Rinnone V Di Marco F Ciuchini, J Clin Microbiol 2007 45 2923 2928 10.1128/JCM.00822-07 17634297 (Pubitemid 47417335)
34. Multiple locus variable number of tandem repeats analysis. G Vergnaud C Pourcel, Methods Mol Biol 2009 551 141 158 full-text 19521873
35. MLVAbank for Bacterial Genotyping. http://mlva.u-psud.fr/