Rapid susceptibility testing for herpes simplex virus type 1 using real-time PCR

Journal of Clinical Virology

Volumn 56, Issue 1, 2013, Pages 19-24

  Van der Beek, Martha T ^a   Claas, Eric C J ^a   van der Blij de Brouwer, Caroline S ^a   Morfin, Florence ^b   Rusman, Lisette G ^a   Kroes, Aloys C M ^a   Vossen, Ann C T M ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b UNIVERSITÉ LYON 1   (France)

Author keywords

Aciclovir; Foscarnet; Herpes simplex virus type 1; Real time PCR; Susceptibility testing

Indexed keywords

ACICLOVIR; FOSCARNET; HEMOGLOBIN BETA CHAIN; VIRUS DNA;

ANTIVIRAL RESISTANCE; ANTIVIRAL SUSCEPTIBILITY; ARTICLE; CELL COUNT; CELL LYSATE; CONTROLLED STUDY; DIAGNOSTIC TEST; DNA DETERMINATION; DNA REDUCTION ASSAY; GENE MUTATION; HERPES SIMPLEX VIRUS 1; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; VALIDATION STUDY; VIRUS CULTURE;

ACYCLOVIR; ANTIVIRAL AGENTS; DNA, VIRAL; FOSCARNET; HERPESVIRUS 1, HUMAN; HUMANS; MICROBIAL SENSITIVITY TESTS; REAL-TIME POLYMERASE CHAIN REACTION; TIME FACTORS;

EID: 84871481198     PISSN: 13866532     EISSN: 18735967     Source Type: Journal    
DOI: 10.1016/j.jcv.2012.09.004     Document Type: Article

References (15)

1. van der Beek M.T., Laheij A.M., Raber-Durlacher J.E., von dem Borne P.A., Wolterbeek R., van der Blij-de Brouwer C.S., et al. Viral loads and antiviral resistance of Herpesviruses and oral ulcerations in hematopoietic stem cell transplant recipients. Bone Marrow Transplant 2012, 47(9):1222-1228. Epub 2012 Feb 13. 10.1038/bmt.2012.2.
2. Burrel S., Deback C., Agut H., Boutolleau D. Genotypic characterization of UL23 thymidine kinase and UL30 DNA polymerase of clinical isolates of herpes simplex virus: natural polymorphism and mutations associated with resistance to antivirals. Antimicrob Agents Chemother 2010, 54:4833-4842.
3. Chibo D., Druce J., Sasadeusz J., Birch C. Molecular analysis of clinical isolates of aciclovir resistant herpes simplex virus. Antiviral Res 2004, 61:83-91.
4. Piret J., Boivin G. Resistance of herpes simplex viruses to nucleoside analogues: mechanisms, prevalence and management. Antimicrob Agents Chemother 2011, 55:459-472.
5. Clinical and Laboratory Standards Institute antiviral susceptibility testing: herpes simplex virus by plaque reduction assay; approved standard M33-A 2004, CLSI, Wayne, PA, USA.
6. Stranska R., van Loon A.M., Polman M., Schuurman R. Application of real-time PCR for determination of antiviral drug susceptibility of herpes simplex virus. Antimicrob Agents Chemother 2002, 46:2943-2947.
7. Thi T.N., Deback C., Malet I., Bonnafous P., Ait-Arkoub Z., Agut H. Rapid determination of antiviral drug susceptibility of herpes simplex virus types 1 and 2 by real-time PCR. Antiviral Res 2006, 69:152-157.
8. Frobert E., Cortay J.C., Ooka T., Najioullah F., Thouvenot D., Lina B., et al. Genotypic detection of aciclovir-resistant HSV-1: characterization of 67 ACV-sensitive and 14 ACV-resistant viruses. Antiviral Res 2008, 79:28-36.
9. Stranska R., van Loon A.M., Polman M., Beersma M.F., Bredius R.G., Lankester A.C., et al. Genotypic and phenotypic characterization of aciclovir-resistant herpes simplex viruses isolated from haematopoietic stem cell transplant recipients. Antivir Ther 2004, 9:565-575.
10. Stranska R., van Loon A.M., Bredius R.G., Polman M., Nienhuis E., Beersma M.F., et al. Sequential switching of DNA polymerase and thymidine kinase-mediated HSV-1 drug resistance in an immunocompromised child. Antivir Ther 2004, 9:97-104.
11. Matrosovich M., Matrosovich T., Garten W., Klenk H.D. New low-viscosity overlay medium for viral plaque assays. Virol J 2006, 3:63.
12. van Doornum G.J., Guldemeester J., Osterhaus A.D., Niesters H.G. Diagnosing herpesvirus infections by real-time amplification and rapid culture. J Clin Microbiol 2003, 41:576-580.
13. Sauerbrei A., Deinhardt S., Zell R., Wutzler P. Phenotypic and genotypic characterization of aciclovir-resistant clinical isolates of herpes simplex virus. Antiviral Res 2010, 86:246-252.
14. Andrei G., Snoeck R., De Clercq E., Esnouf R., Fiten P., Opdenakker G. Resistance of herpes simplex virus type 1 against different phosphonylmethoxyalkyl derivatives of purines and pyrimidines due to specific mutations in the viral DNA polymerase gene. J Gen Virol 2000, 81:639-648.
15. Andrei G., Fiten P., Froeyen M., De C.E., Opdenakker G., Snoeck R. DNA polymerase mutations in drug-resistant herpes simplex virus mutants determine in vivo neurovirulence and drug-enzyme interactions. Antivir Ther 2007, 12:719-732.