Purification, characterization and bioactivity of an extracellular polysaccharide produced from Phellinus igniarius

Annals of Microbiology

Volumn 62, Issue 4, 2012, Pages 1697-1707

  He, Peixin ^a   Geng, Lujing ^a   Wang, Jizhong ^a   Wang, Zheng ^a   Mao, Duobin ^a   Xu, Chunping ^a  

^a ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY   (China)

Author keywords

Chromatography. Fermentation; Exopolysaccharides; Optimization; Phellinus igniarius; Submerged culture

Indexed keywords

PHELLINUS; PHELLINUS IGNIARIUS;

EID: 84871437486     PISSN: 15904261     EISSN: 18692044     Source Type: Journal    
DOI: 10.1007/s13213-012-0427-6     Document Type: Article

References (58)

1. Andersson M, Wittgren B, Wahlund KG (2003) Accuracy in multi-angle light scattering measurements for molar mass and radius estimations. Model calculations and experiments. Anal Chem 75:4279-4291 (Pubitemid 37012566)
2. Bae JT, Sinha J, Park JP, Song CH, Yun JW (2000) Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. J Microbiol Biotechnol 10:482-487
3. Box GEP, Wilson KB (1951) On the experimental attainment of optimum conditions. J R Statist Soc 13:1-45
4. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
5. Chen W, Zhao Z, Chen SF, Li YQ (2008) Optimization for the production of exopolysaccharide from Fomes fomentarius in submerged culture and its antitumor effect in vitro. Bioresour Technol 99:3187-3194 (Pubitemid 351208541)
6. Chihara G, Himuri J, Maeda YY, Arai Y, Fukuoka F (1970) Fraction-ation and purification of the polysaccharides with marked anti-tumor activity, especially, lentinan, from Lentinus edodes (Berk) SING. Cancer Res 30:2776-2781
7. Deepak V, Kalishwaralal K, Ramkumarpandian S, Venkatesh Babu S, Senthilkumar SR, Sangiliyandi G (2008) Optimization of media composition for Nattokinase production by Bacillus subtilis using response surface methodology. Bioresour Technol 99:8170-8174
8. Dong W, Ning L, Lu WD, Li CC, Chen RP, Jia XN, Wang L, Guo LZ (2009) Tumor-inhibitory and liver-protective effects of Phellinus igniarius extracellular polysaccharides. World J Microbiol Bio-technol 25:633-638
9. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350-356
10. Fan L, Soccol AT, Pandey A, Soccol CR (2007) Effect of nutritional and environmental conditions on the production of exo-polysaccharide of Agaricus brasiliensis by submerged fermentation and its antitumor activity. LWT Food Sci Technol 40:30-35 (Pubitemid 44150588)
11. Frankel EN, Meyer AS (2000) The problems of using one-dimensional methods to evaluate multifunctional foods and biological antiox-idants. J Sci Food Agric 80:1925-1941
12. Gong HG, Zhong JJ (2005) Hydrodynamic shear stress affects cell growth and metabolite production by medicinal mushroom Gano-derma lucidum. Chin J Chem Eng 13:426-428
13. Guo X, Zou X, Sun M (2009) Optimization of a chemically defined medium for mycelial growth and polysaccharide production by medicinal mushroom Phellinus igniarius. World J Microbiol Bio-technol 25:2187-2193
14. Han SB, Lee CW, Kang JS, Yoon YD, Lee KH, Lee K, Park SK, Kim HM (2006) Acidic polysaccharide from Phellinus linteus inhibits melanoma cell metastasis by blocking cell adhesion and invasion. Int Immunopharmacol 6:697-702
15. Ho CY, Kim CF, Leung KN, Fung KP, Tse TF, Chan H, Lau CS (2006) Coriolus versicolor (Yunzhi) extract attenuates growth of human leukemia xenografts and induces apoptosis through the mitochondrial pathway. Oncol Reports 16:609-616
16. Hu C, Zhang Y, Kitts DD (2000) Evaluation of antioxidant and prooxidant activities of bamboo Phyllostachys nigra var. henonis leaf extract in vitro. J Agric Food Chem 48:3170-3176
17. Hwang HJ, Kim SW, Choi JW, Yun JW (2003a) Production and characterization of exopolysaccharides from submerged culture of Phellinus linteus KCTC 6190. Enzyme Microb Technol 33:309-319
18. Hwang HJ, Kim SW, Xu CP, Choi JW, Yun JW (2003b) Production and molecular characteristics of four groups of exopolysacchar-ides from submerged culture of Phellinus gilvus. J Appl Microbiol 94:708-719 (Pubitemid 36459560)
19. Ikekawa T (2001) Beneficial effects of edible and medicinal mushrooms in health care. Int J Med Mushrooms 3:291-298
20. Joo JH, Yun JW (2005) Structural and molecular characterization of extracellular polysaccharides produced by a new fungal strain, Trichoderma erinaceum DG-312. J Microbiol Biotechnol 15:1250-1257
21. Jumel K, Fiebrig I, Harding E (1996) Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chro-matography/multi laser light scattering. Int J Biol Macromol 18:133-139 (Pubitemid 26070477)
22. Kim DH, Shim SB, Kim NJ, Jang IS (1999) β-Glucuronidase inhibitory activity and hepatoprotective effect of Ganoderma lucidum. Biol Pharm Bull 22:162-164
23. Kim DH, Yang BK, Jeong SC, Park JB, Cho SP, Das S, Yun JW, Song CH (2001) Production of a hypoglycemic, extracellular polysac-charide from the submerged culture of the mushroom, Phellinus linteus. Biotechnol Lett 23:513-517 (Pubitemid 32299432)
24. Kim SW, Xu CP, Hwang HJ, Choi JW, Kim CW, Yun JW (2003) Production and characterization of exopolysaccharides from an enthomopathogenic fungus Cordycep smilitaris NG3. Biotechnol Progr 19:428-435 (Pubitemid 36425151)
25. Kim SW, Hwang HJ, Choi JW, Yun JW (2005) Characterization of exopolysaccharides produced by submerged culture of an entho-mopathogenic fungus Paecilomyces sinclairii by using a multi-angle laser light scattering system. J Microbiol Biotechnol 15:560-567
26. Kim GY, Lee JY, Lee JO, Ryu CH, Choi BT, Jeong YK, Lee KW, Jeong AC, Choi YH (2006) Partial characterization and immu-nostimulatory effect of a novel polysaccharide-protein complex extracted from Phellinus linteus. Biosci Biotechnol Biochem 70:1218-1226 (Pubitemid 43765255)
27. Lee SY, Kang TS (1996) Production conditions and characterization of the exo-polymer produced by submerged cultivation of Gano-derma lucidum. Korean J Appl Microbiol Biotechnol 24:111-118
28. Lee JH, Cho SM, Kim HM, Hong ND, Yoo ID (1996) Immunostimu-lating activity of polysaccharides from mycelia of Phellinus lin-teus grown under different culture conditions. J Microbiol Biotechnol 6:52-55
29. Lee KM, Lee SY, Lee HY (1999) Bistage control of pH for improving exopolydsaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor. J Biosci Bioeng 99:646-650 (Pubitemid 30046434)
30. Leung PH, Zhao SN, Ho KP, Wu JY (2009) Chemical properties and antioxidant activity of exopolysaccharides from mycelial culture of Cordyceps sinensis fungus Cs-HK1. Food Chem 114:1251-1256
31. Li Y, Weiss WF IV, Roberts CJ (2009) Characterization of high-molecular-weight nonnative aggregates and aggregation kinetics by size exclusion chromatography with inline multi-angle laser light scattering. J Pharm Sci 98:3997-4016
32. Li R, Chen WC, Wang WP, Tian WY, Zhang XG (2010) Antioxidant activity of Astragalus polysaccharides and antitumour activity of the polysaccharides and siRNA. Carbohydr Polym 82:240-244
33. Lim JM, Joo JH, Kim HO, Kim HM, Kim SW, Hwang HJ, Yun JW (2005) Structural analysis and molecular characterization of exo-polysaccharides produced by submerged mycelial culture of Collybia maculata TG-1. Carbohydr Polym 61:296-303 (Pubitemid 41230073)
34. Liu YF, Yang Y, Jia W, Zhang JS, Tang QJ, Tang CH (2006) Determination of total flavones in the medicinal mushroom Phellinus. Acta Edulis Fungi 13:49-52
35. Liu QN, Liu RS, Wang YH, Mi ZY, Li DS, Zhong JJ, Tang YJ (2009) Fed-batch fermentation of Tuber melanosporum for the hyperpro-duction of mycelia and bioactive Tuber polysaccharides. Bioresour Technol 100:3644-3649
36. Liu W, Wang H, Pang X, Yao W, Gao X (2010) Characterization and antioxidant activity of two low-molecular-weight polysaccharides purified from the fruiting bodies of Ganoderma lucidum. Int J Biol Macromol 46:451-457
37. Lung MY, Tsai JC, Huang PC (2010) Antioxidant properties of edible basidiomycete Phellinus igniarius in submerged cultures. J Food Sci 75:E18-E24
38. Mo SY, Yang YC, Shi JG (2003) Studies on chemical constitutes of Phellinus igniarius. China J Chinese Materia Edica 28:339-341
39. Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA (2007) Immu-nonodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). Int Immunopharmacol 7:701-724 (Pubitemid 46635272)
40. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63 (Pubitemid 14203433)
41. Park JP, Kim SW, Hwang HJ, Yun JW(2001) Optimizationof submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris. Lett Appl Microbiol 33:76-81 (Pubitemid 32625737)
42. Rashid S, Unyayar A, Mazmanci MA, McKeown SR, Banat IM, Worthington J (2011) A study of anti-cancer effects of Funalia trogii in vitro and in vivo. Food Chem Toxicol 49:1477-1483
43. Rodriguez Estrada AE, Jimenez-Gasco Mdel M, Royse DJ (2009) Improvement of yield of Pleurotus eryngii var. eryngii by substrate supplementation and use of a casing overlay. Bioresour Technol 100:5270-5276
44. Sakanaka S, Tachibana Y, Okada Y (2005) Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (kakinoha-cha). Food Chem 89:569-575 (Pubitemid 39221380)
45. Shon YH, Nam KS (2004) Inhibition of cytochrome P450 isozymes and ornithine decarboxylase activities by polysaccharides from soybeans fermented with Phellinus igniarius or Agrocybe cylin-dracea. Biotechnol Lett 26:159-163
46. Sliva D, Kawasaki J, Stanley G, Harvey K, Slivova V (2006) Phellinus linteus inhibits growth and invasive behavior of breast cancer cells through the suppression of Akt signaling. FASEB J 20:559-560
47. Song CH, Jeon YJ, Yang BK, Ra KS, Sung JM (1998) Anti-complementary activity of exo-polymers produced from submerged mycelial cultures of higher fungi with particular reference to Cordyceps militaris. J Microbiol Biotechnol 8:536-539
48. Song TY, Lin HC, Yang NC, Hu ML (2008) Antiproliferative and antimetastatic effects of the ethanolic extract of Phellinus igniar-ius (Linnearus: Fries) Quelet. J Ethnopharmacol 115:50-56
49. Tabata K, Itoh W, Kojima T, Kawabata S, Misaki A (1981) Ultrasonic degradation of schizophyllan, an antitumor polysaccharide produced by Schizophyllum commune FRIES. Carbohyd Res 89:121-135
50. Tang YJ, Zhang W, Zhong JJ (2009) Performance analyses of a pH-shift and DOT-shift integrated fed-batch fermentation process for the production of ganoderic acid and Ganoderma polysaccharides by medicinal mushroom Ganoderma lucidum. Bioresour Technol 100:1852-1859
51. Tao Y, Zhang L, Cheung PCK (2006) Physicochemical properties and antitumor activities of water-soluble native and sulfated hyper-branched mushroom polysaccharides. Carbohyd Res 341:2261-2269 (Pubitemid 44092677)
52. Wasser S (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60:258-274 (Pubitemid 35352092)
53. Wyatt PJ (1993) Light scattering and the absolute characterization of macromolecules. Anal Chim Acta 272:1-40 (Pubitemid 23026256)
54. Yang Y, Zhang JS, Liu YF, Tang QJ, Zhao ZG, Wen SX (2007) Structural elucidation of a 3-O-methyl-D-galactose-containing neutral polysaccharide from the fruiting bodies of Phellinus igniarius. Carbohydr Res 342:1063-1070 (Pubitemid 46551993)
55. Zhang M, Cui SW, Cheung PCK, Wang Q (2007) Antitumor poly-saccharides from mushrooms: a review on their isolation process, structural characteristics and antitumor activity. Trends Food Sci Technol 18:4-19 (Pubitemid 44827599)
56. Zhao J, Gui LN, Sun JM, Cao Y, Zhang H (2010) Antioxidative activity of polysaccharide fractions isolated from Tricholoma Matsutake sing. with ultrafiltration. J Life Sci 4:17-20
57. Zhou B, Jia L, Meng FY, Song Z, Liu XN, Deng P, Fan KM (2010) Statistical optimization of cultivation conditions for exopoly-sacchride production and mycelia growth by Stropharia rugo-soannulata. Ann Micorobiol 60:89-96
58. Zhu H, Sun S, Zhang S (2011) Enhanced production of total flavones and exopolysaccharides via Vitreoscilla hemoglobin biosynthesis in Phellinus igniarius. Bioresour Technol 102:1747-1751