cDNA normalization by hydroxyapatite chromatography to enrich transcriptome diversity in RNA-seq applications

BioTechniques

Volumn 53, Issue 6, 2012, Pages 373-380

  VanderNoot, Victoria A ^a   Langevin, Stanley A ^a   Solberg, Owen D ^a   Lane, Pamela D ^a   Curtis, Deanna J ^a   Bent, Zachary W ^a   Williams, Kelly P ^a   Patel, Kamlesh D ^a   Schoeniger, Joseph S ^a   Branda, Steven S ^a   Lane, Todd W ^a  

^a SANDIA NATIONAL LABORATORIES   (United States)

Author keywords

C0t filtration; Hydroxyapatite chromatography; Microcolumn; Nucleic acid normalization; RNA seq; rRNA depletion; Second generation sequencing

Indexed keywords

COMPLEMENTARY DNA; DOUBLE STRANDED DNA; HYDROXYAPATITE; RIBOSOME RNA; TRANSCRIPTOME;

CHROMATOGRAPHY; CONTROLLED STUDY; DNA LIBRARY; GENE MAPPING; HUMAN; HUMAN CELL; HYDROXYAPATITE CHROMATOGRAPHY; POLYMERASE CHAIN REACTION; REVIEW; RNA SEQUENCE; RNA TRANSCRIPTION;

BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; CHROMOSOME MAPPING; DURAPATITE; ESCHERICHIA COLI K12; GENE LIBRARY; HUMANS; LEUKOCYTES, MONONUCLEAR; RNA; SEQUENCE ANALYSIS, RNA; TRANSCRIPTOME;

ESCHERICHIA COLI K12; EUKARYOTA; PROKARYOTA;

EID: 84871349680     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/000113937     Document Type: Review

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