Rare target enrichment for ultrasensitive PCR detection using cot-rehybridization and duplex-specific nuclease

Analytical Biochemistry

Volumn 421, Issue 1, 2012, Pages 81-85

  Gijavanekar, Charul ^a,e   Strych, Ulrich ^a   Fofanov, Yuriy ^a,b,c   Fox, George E ^a,d   Willson, Richard C ^a,c,d  

^a University of Houston   (United States)

^b University of Houston   (United States)

^c HOUSTON METHODIST RESEARCH INSTITUTE   (United States)

^d UNIVERSITY OF HOUSTON   (United States)

^e BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Cot analysis; Cot rehybridization; Duplex specific nuclease; PCR diagnostics; PCR sensitivity; Target enrichment

Indexed keywords

BIOMOLECULES; DNA SEQUENCES;

BACKGROUND SUPPRESSION; COMPLEX BACKGROUND; COT ANALYSIS; DUPLEX-SPECIFIC NUCLEASE; NUCLEIC ACID DETECTION; PCR SENSITIVITY; RE-HYBRIDIZATION; SENSITIVITY AND SPECIFICITY;

POLYMERASE CHAIN REACTION;

DNA FRAGMENT; DOUBLE STRANDED DNA; NUCLEIC ACID;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; DNA SEQUENCE; ENRICHMENT CULTURE; HYBRIDIZATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

DECAPODA (CRUSTACEA);

EID: 84855877011     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.11.010     Document Type: Article

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