Increased number of micronuclei and nuclear anomalies in buccal mucosa cells from people exposed to alcohol-containing mouthwash

Drug and Chemical Toxicology

Volumn 36, Issue 2, 2013, Pages 255-260

  Zamora Perez, Ana Lourdes ^a   Mariaud Schmidt, Rocío Patricia ^a   Fuentes Lerma, Martha Graciela ^a   Guerrero Velázquez, Celia ^a   Gómez Meda, Belinda Claudia ^a   López Verdín, Sandra ^a   Zúñiga González, Guillermo Moisés ^b  

^a UNIVERSITY OF GUADALAJARA   (Mexico)

^b INSTITUTO MEXICANO DEL SEGURO SOCIAL   (Mexico)

Author keywords

Cytotoxicity; Ethanol; Genotoxicity; Mouthwash

Indexed keywords

ALCOHOL; MOUTHWASH;

ADULT; ARTICLE; CELL COUNT; CHEEK MUCOSA; CHROMATIN; CONTROLLED STUDY; CYTOTOXICITY; DNA DAMAGE; FEMALE; HUMAN; HUMAN CELL; MALE; MICRONUCLEUS; MICROSCOPY; MOUTH MUCOSA; MUCOSA CELL; POPULATION EXPOSURE;

ADULT; CELL NUCLEUS; DNA DAMAGE; ETHANOL; FEMALE; HUMANS; MALE; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; MOUTH MUCOSA; MOUTHWASHES; YOUNG ADULT;

EID: 84871232134     PISSN: 01480545     EISSN: 15256014     Source Type: Journal    
DOI: 10.3109/01480545.2012.737804     Document Type: Article

References (29)

1. Bolanowski, S. J., Gescheider, G. A., Sutton, S. V. (1995). Relationship between oral pain and ethanol concentration in mouth rinses. J Periodontal Res 130:192-197.
2. Bonassi, S., Biasotti, B., Kirsch-Volders, M., Knasmueller, S., Zeiger, E., Burgaz, S., et al.; HUMNXL Project Consortium. (2009). State of the art survey of the buccal micronucleus assay - A first stage in the HUMN(XL) project initiative. Mutagenesis 24:295-302.
3. Bonassi, S., Coskun, E., Ceppi, M., Lando, C., Bolognesi, C., Burgaz, S., et al. (2011). The Human micronucleus project on exfoliated buccal cells (HUMN(XL)): The role of life-style, host factors, occupational exposures, health status, and assay protocol. Mutat Res 728:88-97.
4. Carlin, V., Matsumoto, M. A., Saraiva, P. P., Artioli, A., Oshima, C. T., Ribeiro, D. A. (2012). Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro. Clin Oral Investig 16:813-820.
5. Carretero-Peláez, M. A., Esparza-Gómez, G. C., Figuero-Ruiz, E. (2004). Alcohol containing mouthwashes and oral cancer. Critical analysis of literature. Med Oral 9:116-123.
6. Ceppi, M., Biasotti, B., Fenech, M., Bonassi, S. (2010). Human population studies with the exfoliated buccal micronucleus assay: Statistical and epidemiological issues. Mutat Res 705:11-19.
7. Heddle, J. A., Cimino, M. C., Hayashi, M., Romagna, F., Shelby, M. D., Tucker, J. D., et al. (1991). Micronuclei as an index of cytogenetic damage: Past, present, and future. Environ Mol Mutagen 18:277-291.
8. Holland, N., Bolognesi, C., Kirsch-Volders, M., Bonassi, S., Zeiger, E., Knasmueller, S., et al. (2008). The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: The HUMN project perspective on current status and knowledge gaps. Mutat Res 659:93-108.
9. IARC. (2010). IARC monographs on the evaluation of carcinogenic risks to humans, vol. 96, alcoholic beverage consumption and ethyl carbamate (urethane). Lyon, France: International Agency for Research on Cancer
10. Ishikawa, H., Ishikawa, T., Yamamoto, H., Fukao, A., Yokoyama, K. (2007). Genotoxic effects of alcohol in human peripheral lymphocytes modulated by ADH1B and ALDH2 gene polymorphisms. Mutat Res 615:134-142.
11. Kuyama, K., Yamamoto, H. (1997). A study of effects of mouthwash on the human oral mucosa: With special references to sites, sex differences, and smoking. J Nihon Univ Sch Dent 39:202-210.
12. La Vecchia, C. (2009). Mouthwash and oral cancer risk: An update. Oral Oncol 45:198-200.
13. Lachenmeier, D. W. (2008). Safety evaluation of topical applications of ethanol on the skin and inside the oral cavity. J Occup Med Toxicol 13:3-26.
14. Lachenmeier, D. W., Gumbel-Mako, S., Sohnius, E. M., Keck-Wilhelm, A., Kratz, E., Mildau, G. (2009). Salivary acetaldehyde increase due to alcohol-containing mouthwash use: A risk factor for oral cancer. Int J Cancer 125:730-735.
15. Maffei, F., Fimognari, C., Castelli, E., Stefanini, G. F., Forti, G. C., Hrelia, P. (2000). Increased cytogenetic damage detected by FISH analysis on micronuclei in peripheral lymphocytes from alcoholics. Mutagenesis 15:517-523.
16. McCullough, M. J., Farah, C. S. (2008). The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes. Aust Dent J 53:302-305.
17. Moazzez, R., Thompson, H., Palmer, R. M., Wilson, R. F., Proctor, G. B., Wade, W. G. (2011). Effect of rinsing with ethanol-containing mouthrinses on the production of salivary acetaldehyde. Eur J Oral Sci 119:441-446.
18. Poggi, P., Rodriguez y Baena, R., Rizzo, S., Rota, M. T. (2003). Mouthrinses with alcohol: Cytotoxic effects on human gingival fibroblasts in vitro. J Periodontol 74:623-629.
19. Reis, S. R., do Espírito Santo, A. R., Andrade, M. G., Sadigursky, M. (2006). Cytologic alterations in the oral mucosa after chronic exposure to ethanol. Braz Oral Res 20:97-102.
20. Rodrigues, F., Lehmann, M., do Amaral, V. S., Reguly, M. L., de Andrade, H. H. (2007). Genotoxicity of three mouthwash products, Cepacol, Periogard, and Plax, in the Drosophila wing-spot test. Environ Mol Mutagen 48:644-649.
21. Seitz, H. K., Becker, P. (2007). Alcohol metabolism and cancer risk. Alcohol Res Health 30:38-41.
22. Thomas, P., Holland, N., Bolognesi, C., Kirsch-Volders, M., Bonassi, S., Zeiger, E., et al. (2009). Buccal micronucleus cytome assay. Nat Protoc 4:825-837.
23. Torres-Bugarín, O., Covarrubias-Bugarín, R., Zamora-Perez, A. L., Torres-Mendoza, B. M., García-Ulloa, M., Martínez-Sandoval, F. G. (2007). Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders. Br J Sports Med 41:592-596.
24. Torres-Bugarín, O., Ventura-Aguilar, A., Zamora-Perez, A., Gómez- Meda, B. C., Ramos-Ibarra, M. L., Morga-Villela, G., et al. (2004). Evaluation of cisplatin + 5-FU, carboplatin + 5-FU, and ifosfamida + epirubicine regimens using the micronuclei test and nuclear abnormalities in the buccal mucosa. Mutat Res 565:91-101.
25. Werner, C. W., Seymour, R. A. (2009). Are alcohol containing mouthwashes safe? Br Dent J 207:488-489.
26. Wight, A. J., Ogden, G. R. (1988). Possible mechanisms by which alcohol may influence the development of oral cancer - A review. Oral Oncol 34:442-447.
27. Winn, D. M., Blot, W. J., McLaughlin, J. K., Austin, D. F., Greenberg, R. S., Preston-Martin, S., et al. (1991). Mouthwash use and oral conditions in the risk of oral and pharyngeal cancer. Cancer Res 51:3044-3047.
28. Wynder, E. L., Kabat, G., Rosenberg, S., Levenstein, M. (1983). Oral cancer and mouthwash use. J Natl Cancer Inst 70:255-260.
29. Zúñiga-González, G. M., Batista-González, C. M., Gómez-Meda, B. C., Ramos-Ibarra, M. L., Zamora-Perez, A. L., Muñoz-Magallanes, T., et al. (2007). Micronuclei in diabetes: Folate supplementation diminishes micronuclei in diabetic patients but not in an animal model. Mutat Res 634:126-134.