Mouthrinses with alcohol: Cytotoxic effects on human gingival fibroblasts in vitro

Journal of Periodontology

Volumn 74, Issue 5, 2003, Pages 623-629

  Poggi, Paola ^a   Rodriguez y Baena, Ruggero ^b   Rizzo, Silvana ^b   Rota, Massimo T ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b UNIVERSITY OF PAVIA   (Italy)

Author keywords

Ethanol metabolism; Fibroblasts, gingival; Mouthwashes adverse effects; Periodontal diseases therapy

Indexed keywords

ACETALDEHYDE; ALCOHOL;

ARTICLE; CELL ADHESION; CELL STRUCTURE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOSKELETON; CYTOTOXICITY; DOSE TIME EFFECT RELATION; FIBROBLAST; FIBROBLAST CULTURE; GINGIVA; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; INCUBATION TIME; MOUTH; MOUTH HYGIENE; MOUTHWASH; TRANSMISSION ELECTRON MICROSCOPY;

ACETALDEHYDE; ADULT; ANTI-INFECTIVE AGENTS, LOCAL; CELL ADHESION; CELL SURVIVAL; CELLS, CULTURED; CYTOSKELETON; DOSE-RESPONSE RELATIONSHIP, DRUG; ETHANOL; EXCIPIENTS; FIBROBLASTS; GINGIVA; HUMANS; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; MOUTHWASHES; ORGANELLES; TIME FACTORS;

EID: 0037838798     PISSN: 00223492     EISSN: None     Source Type: Journal    
DOI: 10.1902/jop.2003.74.5.623     Document Type: Article

References (28)

1. Bhatty SA, Walsh TF, Douglas CW. Ethanol and pH levels of proprietary mouthrinses. Community Dent Health 1994;11:71-74.
2. Bolanowski SJ, Gescheider GA, Sutton SV. Relationship between oral pain and ethanol concentration in mouthrinses. J Periodont Res 1995;30:192-197.
3. Ciancio SG. Agents for the management of plaque and gingivitis. J Dent Res 1992;71:1450-1454.
4. Shulman JD, Wells LM. Acute ethanol toxicity from ingesting mouthwash in children younger than 6-years of age. Pediatr Dent 1997;19:404-408.
5. Lieber CS. Metabolic effects of acetaldehyde. Biochem Soc Trans 1988;16:241-248.
6. Dong YJ, Peng TK, Yin SJ. Expression and activities of class IV alcohol dehydrogenase and class III aldehyde dehydrogenase in human mouth. Alcohol 1996;13:257-262.
7. Vaikevainen S, Tillonen J, Agarwal DP, Srivastava N, Salaspuro M. High salivary acetaldehyde after a moderate dose of alcohol in ALDH-2 deficient subjects: Strong evidence for the local carcinogenic action of acetaldehyde. Alcohol Clin Exp Res 2000;24:872-877.
8. Giannopoulou C, Cimasoni G. Functional characteristics of gingival and periodontal ligament fibroblasts. J Dent Res 1996;75:895-902.
9. Somerman MJ, Archer SY, Imm GR, Foster RA. A comparative study of human periodontal ligament cells and gingival fibroblasts in vitro. J Dent Res 1988;67:66-70.
10. Jokelainen K, Heikkonen E, Roine R, Lehtonen H, Salaspuro M. Increased acetaldehyde production by mouthwashings from patients with oral cavity, laryngeal, or pharyngeal cancer. Alcohol Clin Exp Res 1996;20: 1206-1210.
11. Kuenen-Boumeester V, van Loenen P, de Bruijn E, Henzen-Logmans SC. Quality control of immunocytochemical staining of effusions using a standardized method of cell processing. Acta Cytol 1996;40:475-479.
12. Szabo G. Consequences of alcohol consumption on host defence. Alcohol Alcohol 1999;34:830-841.
13. Christen AG. Dentistry and the alcoholic patient. Dent Clin North Am 1983;27:341-361.
14. Slomiany BL, Piotrowsky J, Slomiany A. Suppression of endothelin-converting enzyme-1 during buccal mucosal ulcer healing: Effect of chronic alcohol ingestion. Biochem Biophys Res Commun 2000;271:318-322.
15. Gottsegen R. Diabetes mellitus, cardiovascular diseases, and alcoholism. In: Schluger S, Yuodelis R, Page RC, Johnson RH, eds. Periodontal Diseases, 2nd ed. Philadelphia: Lea & Febiger; 1993:273-283.
16. Tezal M, Grossi SG, Ho AW, Genco RJ. The effect of alcohol consumption on periodontal disease. J Periodontol 2001;72:183-189.
17. Espina N, Lima V, Lieber CS, Garro AJ. In vitro and in vivo inhibitory effect of ethanol and acetaldehyde on O6-methylguanine transferase. Carcinogenesis 1988;9: 761-766.
18. Grafstrom RC, Dypbukt JM, Sundqvist K, et al. Pathobiological effects of acetaldehyde in cultured human epithelial cells and fibroblasts. Carcinogenesis 1994;15: 985-990.
19. Vaca CE, Nilsson JA, Fang JL, Grafstrom RC. Formation of DNA adducts in human buccal epithelial cells exposed to acetaldehyde and methylglyoxal in vitro. Chem Biol Interact 1998;108:197-208.
20. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. In: Molecular Biology of the Cell. New York: Garland Publishing Inc.; 1994:593-605.
21. Matsuda Y, Baraona E, Salaspuro M, Lieber CS. Effects of ethanol on liver microtubules and Golgi apparatus. Possible role of altered hepatic protein secretion of plasma protein. Lab Invest 1979;41:455-463.
22. Baraona E, Matsuda Y, Pikkarainen P, Finkelman F, Lieber CS. Effects of ethanol on hepatic protein secretion and microtubules. Possible mediation of acetaldehyde. Curr Alcohol 1981;8:421-434.
23. Lieber CS. Microsomal ethanol-oxidizing system (MEOS): The first 30 years (1968-1998). A review. Alcohol Clin Exp Res 1999;23:991-1007.
24. Redman CM, Banerjee D, Howell K, Palade GE. Colchicine inhibition of plasma protein release from rat hepatocytes. J Cell Biol 1975;66:42-48.
25. Redman CM, Banerjee D, Manning C, Huang CY, Green K. In vivo effect of colchicine on hepatic protein synthesis and on the conversion of proalbumin to serum albumin. J Cell Biol 1978;77:400-405.
26. Arwailer NB, Netuschil L, Reich E. Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality. A controlled clinical study. J Clin Periodontol 2001;28:168-174.
27. Leyes-Borrajo JL, Garcia VL, Lopez CG, Rodriguez-Nunez I, Garcia FM, Gallas TM. Efficacy of chlorhexidine mouthrinses with and without alcohol: A clinical study. J Periodontol 2002;73:317-321.
28. Mascarenhas AK, Allen CM, Loudon J. The association between Viadent use and oral leukoplakia. Epidemiology 2001;12:741-743.