Promiscuous contacts and heightened dynamics increase thermostability in an engineered variant of the engrailed homeodomain

Protein Engineering, Design and Selection

Volumn 26, Issue 1, 2013, Pages 35-45

  McCully, Michelle E ^a,b   Beck, David A C ^b,c   Daggett, Valerie ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b University of Washington   (United States)

^c University of Washington   (United States)

Author keywords

molecular dynamics; protein engineering; simulation validation; thermostabilized proteins

Indexed keywords

BACKBONE DYNAMICS; DYNAMIC PROPERTY; EXPLICIT SOLVENTS; HOMEODOMAIN; J COUPLING; NATIVE CONFORMATION; NATIVE STATE; NATIVE STRUCTURES; NUCLEAR MAGNETIC RESONANCE DATA; NUCLEAR OVERHAUSER EFFECTS; ORDER PARAMETER; PROTEIN DYNAMICS; PROTEIN ENGINEERING; ROOM TEMPERATURE; SEQUENCE IDENTITY; SIDE-CHAINS; SIMULATION VALIDATION; WILD TYPES; WILD-TYPE PROTEINS;

AMINO ACIDS; GENETIC ENGINEERING; MOLECULAR DYNAMICS; NUCLEAR MAGNETIC RESONANCE;

PROTEINS;

HOMEODOMAIN PROTEIN;

ARTICLE; CORRELATION ANALYSIS; CRYSTAL STRUCTURE; HYDROGEN BOND; HYDROPHOBICITY; MELTING POINT; MOLECULAR DYNAMICS; NUCLEAR MAGNETIC RESONANCE; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN INTERACTION; PROTEIN STRUCTURE; ROOM TEMPERATURE; THERMOSTABILITY;

AMINO ACID SEQUENCE; HOMEODOMAIN PROTEINS; MOLECULAR DYNAMICS SIMULATION; MOLECULAR SEQUENCE DATA; MOVEMENT; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 84871198117     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs063     Document Type: Article

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