Quantitative ELISAs for serum soluble LHCGR and hCG-LHCGR complex: Potential diagnostics in first trimester pregnancy screening for stillbirth, Down's syndrome, preterm delivery and preeclampsia

Reproductive Biology and Endocrinology

Volumn 10, Issue , 2012, Pages

  Chambers, Anne E ^a,e   Griffin, Christopher ^b   Naif, Samantha A ^c   Mills, Ian ^c   Mills, Walter E ^a,e   Syngelaki, Argyro ^d   Nicolaides, Kypros H ^d   Banerjee, Subhasis ^a,e  

^a BIRMINGHAM HEARTLANDS HOSPITAL   (United Kingdom)

^b MUMS Clinic   (United Kingdom)

^c BIRMINGHAM WOMEN S HOSPITAL   (United Kingdom)

^d KING S COLLEGE HOSPITAL   (United Kingdom)

^e Origin Biomarkers   (United Kingdom)

Author keywords

Down's syndrome; Early pregnancy; ELISA; LHCGR; Preeclampsia; Preterm delivery; Stillbirth

Indexed keywords

CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN ANTIBODY; CHORIONIC GONADOTROPIN BETA SUBUNIT; EPITOPE; LUTEINIZING HORMONE RECEPTOR; MONOCLONAL ANTIBODY; MULTIPROTEIN COMPLEX; POLYCLONAL ANTIBODY; PREGNANCY ASSOCIATED PLASMA PROTEIN A; RECEPTOR ANTIBODY; RECOMBINANT RECEPTOR;

AMINO ACID SEQUENCE; ANEUPLOIDY; ANIMAL CELL; ARTICLE; BIOCHEMISTRY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DOWN SYNDROME; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; FIRST TRIMESTER PREGNANCY; HORMONE BLOOD LEVEL; HORMONE RECEPTOR INTERACTION; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PREECLAMPSIA; PREGNANCY OUTCOME; PREMATURE LABOR; PRENATAL SCREENING; QUANTITATIVE ASSAY; SENSITIVITY AND SPECIFICITY; STILLBIRTH; VALIDATION PROCESS;

ADULT; ANTIBODIES, MONOCLONAL; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; DOWN SYNDROME; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; FETAL DISEASES; HUMANS; PRE-ECLAMPSIA; PREGNANCY; PREGNANCY OUTCOME; PREGNANCY TRIMESTER, FIRST; PREGNANCY-ASSOCIATED PLASMA PROTEIN-A; PREMATURE BIRTH; PRENATAL DIAGNOSIS; PROSPECTIVE STUDIES; RECEPTORS, LH; REPRODUCIBILITY OF RESULTS; RETROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; STILLBIRTH;

EID: 84871004707     PISSN: None     EISSN: 14777827     Source Type: Journal    
DOI: 10.1186/1477-7827-10-113     Document Type: Article

References (42)

1. Licht P, Russu V, Wildt L. On the role of human chorionic gonadotropin (hCG) in the embryo-endometrial microenvironment: implications for differentiation and implantation. Semin Reprod Med 2001, 19:37-47. 10.1055/s-2001-13909, 11394202.
2. Rahman NA, Rao CV. Recent progress in luteinizing hormone/human chorionic gonadotrophin hormone research. Mol Hum Reprod 2009, 15:703-711. 10.1093/molehr/gap067, 19710244.
3. Schubert RL, Narayan P, Puett D. Specificity of cognate ligand-receptor interactions: fusion proteins of human chorionic gonadotropin and the heptahelical receptors for human luteinizing hormone, thyroid-stimulating hormone, and follicle-stimulating hormone. Endocrinology 2003, 144(1):129-137. 10.1210/en.2002-220829, 12488338.
4. Glinoer D, De Nayer P, Bourdoux P, Lemone M, Robyn C, Van Steirteghem A, Kinthaert J, Lejeune B. Regulation of maternal thyroid during pregnancy. J Clin Endocrinol Metab 1990, 71:276-287. 10.1210/jcem-71-2-276, 2116437.
5. Atger M, Misrahi M, Sar S, Le FL, Dessen P, Milgrom E. Structure of the human luteinizing hormone-choriogonadotropin receptor gene: unusual promoter and 5' non-coding regions. Mol Cell Endocrinol 1995, 111:113-123. 10.1016/0303-7207(95)03557-N, 7556872.
6. Madhra M, Gay E, Fraser HM, Duncan WC. Alternative splicing of the human luteal LH receptor during luteolysis and maternal recognition of pregnancy. Mol Hum Reprod 2004, 10:599-603. 10.1093/molehr/gah076, 15169923.
7. Kolena J, Seböková E. Porcine follicular fluid containing water-soluble LH/hCG receptor. Arch Int Physiol Biochim 1986, 94:261-270. 10.3109/13813458609085343, 2439047.
8. West AP, Cooke BA. Regulation of the truncation of luteinizing hormone receptors at the plasma membrane is different in rat and mouse Leydig cells. Endocrinology 1991, 128:363-370. 10.1210/endo-128-1-363, 1846104.
9. Tsai-Morris CH, Buczko E, Wang W, Dufau ML. Intronic nature of the rat luteinizing hormone receptor gene defines a soluble receptor subspecies with hormone binding activity. J Biol Chem 1990, 265:19385-19398.
10. VuHai-LuuThi MT, Misrah M, Houllier A, Jolivet A, Milgrom E. Variant forms of the pig lutropin/choriogonadotropin receptor. Biochemistry 1992, 31:8377-8383. 10.1021/bi00150a035, 1326331.
11. Remy JJ, Nespoulou C, Grosclaude J, Grébert D, Couture L, Pajot E, Salesse R. Purification and structural analysis of a soluble human chorionogonadotropin hormone-receptor complex. J Biol Chem 2001, 276:1681-1687.
12. Chambers AE, Stanley PF, Randeva H, Banerjee S. Microvesicle-mediated release of soluble LH/hCG receptor (LHCGR) from transfected cells and placenta explants. Reprod Biol Endocrinol 2011, 15:9-64.
13. Dunkel L, Raivio T, Laine J, et al. Circulating luteinizing hormone receptor inhibitor(s) in boys with chronic renal failure. Kidney Int 1997, 51:777-784. 10.1038/ki.1997.109, 9067910.
14. Chambers AE, Nayini KP, Mills WE, Lockwood GM, Banerjee S. Circulating LH/hCG receptor (LHCGR) may identify pre-treatment IVF patients at risk of OHSS and poor implantation. Reprod Biol Endocrinol 2011, 23:9-161.
15. Lobel LI, Pollak S, Klein J, Lustbader JW. High-level bacterial expression of a natively folded, soluble extracellular domain fusion protein of the human luteinizing hormone/chorionic gonadotropin receptor in the cytoplasm of Escherichia coli. Endocrine 2001, 14(2):205-212. 10.1385/ENDO:14:2:205, 11394638.
16. Fralish GB, Narayan P, Puett D. High-level expression of a functional single-chain human chorionic gonadotropin-luteinizing hormone receptor ectodomain complex in insect cells. Endocrinology 2001, 142(4):1517-1524. 10.1210/en.142.4.1517, 11250932.
17. Meduri G, Charnaux N, Loosfelt H, Jolivet A, Spyratos F, Brailly S, Milgrom E. Luteinizing hormone/ human chorionic gonadotropin receptors in breast cancer. Cancer Res 1997, 57(5):857-864.
18. Banerjee S, Smallwood A, Chambers AE, Papageorghio A, Loosfelt H, Spencer K, Campbell S, Nicolaides K. A link between high serum levels of human chorionic gonadotrophin and chorionic expression of its mature functional receptor (LHCGR) in Down's syndrome pregnancies. Reprod Biol Endocrinol 2005, 3:25. 10.1186/1477-7827-3-25, 1190215, 15969756.
19. Pidoux G, Gerbaud P, Tsatsaris V, Marpeau O, Ferreira F, Meduri G, Guibourdenche J, Badet J, Evain-Brion D, Frendo JL. Biochemical characterization and modulation of LH/CG-receptor during human trophoblast differentiation. J Cell Physiol 2007, 212(1):26-35. 10.1002/jcp.20995, 17458905.
20. Angelova K, Narayan P, Puett D. The luteinizing hormone receptor: influence of buffer composition on ligand binding and signaling of wild type and mutant receptors. Mol Cell Endocrinol 2003, 204(1-2):1-9.
21. Kagan KO, Wright D, Valencia C, Maiz N, Nicolaides KH. Screening for trisomies 21, 18 and 13 by maternal age, fetal nuchal translucency, fetal heart rate, free beta-hCG and pregnancy-associated plasma protein-A. Hum Reprod 2008, 23(9):1968-1975. Epub 2008 Jun 10, 10.1093/humrep/den224, 18544579.
22. Banerjee P, Fazleabas AT. Extragonadal actions of chorionic gonadotropin. Rev Endocr Metab Disord 2011, 12(4):323-332. 10.1007/s11154-011-9193-1, 3208730, 21845366.
23. Spencer K, Cowans NJ, Nicolaides KH. Low levels of maternal serum PAPP-A in the first trimester and the risk of pre-eclampsia. Prenat Diagn 2008, 28(1):7-10. 10.1002/pd.1890, 18000943.
24. Pidoux G, Gerbaud P, Marpeau O, Guibourdenche J, Ferreira F, Badet J, Evain-Brion D, Frendo JL. Human placental development is impaired by abnormal human chorionic gonadotropin signaling in trisomy 21 pregnancies. Endocrinology 2007, 148:5403-5413. 10.1210/en.2007-0589, 17690166.
25. Kalinderis M, Papanikolaou A, Kalinderi K, Ioannidou E, Giannoulis C, Karagiannis V, Tarlatzis BC. Elevated serum levels of interleukin-6, interleukin-1β and human chorionic gonadotropin in pre-eclampsia. Am J Reprod Immunol 2011, 66(6):468-475. 10.1111/j.1600-0897.2011.01019.x, 21623995.
26. Kharfi A, Giguère Y, De Grandpré P, Moutquin JM, Forest JC. Human chorionic gonadotropin (hCG) may be a marker of systemic oxidative stress in normotensive and preeclamptic term pregnancies. Clin Biochem 2005, 38(8):717-721. 10.1016/j.clinbiochem.2005.04.011, 15904911.
27. Gurbuz A, Karateke A, Mengulluoglu M, Gedikbasi A, Ozturkmen M, Kabaca C, Sahinoglu Z. Can serum HCG values be used in the differential diagnosis of pregnancy complicated by hypertension?. Hypertens Pregnancy 2004, 23(1):1-12. 10.1081/PRG-120028276, 15612109.
28. Shenhav S, Gemer O, Sassoon E, Volodarsky M, Peled R, Segal S. Mid-trimester triple test levels in early and late onset severe pre-eclampsia. Prenat Diagn 2002, 22(7):579-582. 10.1002/pd.365, 12124692.
29. Casart YC, Camejo MI, Proverbio F, Febres F. Bioactivity of serum hCG in preeclampsia. Obstet Gynecol 2001, 98(3):463-465. 10.1016/S0029-7844(01)01463-6, 11530130.
30. Zygmunt M, Herr F, Keller-Schoenwetter S, Kunzi-Rapp K, Munstedt K, Rao CV, Lang U, Preissner KT. Characterization of human chorionic gonadotropin as a novel angiogenic factor. J Clin Endocrinol Metab 2002, 87:5290-5296. 10.1210/jc.2002-020642, 12414904.
31. Berndt S, Perrier D'Hauterive S, Blacher S, Péqueux C, Lorquet S, Munaut C, Applanat M, Hervé MA, Lamandé N, Corvol P, Van Den Brûle F, Frankenne F, Poutanen M, Huhtaniemi I, Geenen V, Noël A, Foidart JM. Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium. FASEB J 2006, 20(14):2630-2632. Epub 2006 Oct 25, 10.1096/fj.06-5885fje, 17065221.
32. Berndt S, Blacher S, Perrier D'Hauterive S, Thiry M, Tsampalas M, Cruz A, Péqueux C, Lorquet S, Munaut C, Noël A, Foidart JM. Chorionic gonadotropin stimulation of angiogenesis and pericyte recruitment. Clin Endocrinol Metab 2009, 94(11):4567-4574. Epub 2009 Oct 16.
33. Lockwood CM, Grenache DG, Gronowski AM. Serum human chorionic gonadotropin concentrations greater than 400,000 IU/L are invariably associated with suppressed serum thyrotropin concentrations. Thyroid 2009, 19:863-868. 10.1089/thy.2009.0079, 19505185.
34. Haddow JE, McClain MR, Lambert-Messerlian G, Palomaki GE, Canick JA, Cleary-Goldman J, Malone FD, Porter TF, Nyberg DA, Bernstein P, D'Alton ME. First and second trimester evaluation of risk for fetal aneuploidy research consortium. Variability in thyroid-stimulating hormone suppression by human chorionic [corrected] gonadotropin during early pregnancy. J Clin Endocrinol Metab 2008, 93(9):3341-3347. 10.1210/jc.2008-0568, 2567848, 18544616.
35. Hidaka A, Ban T, Panesar NS, Minegishi T, Kohn LD, Tahara K. Thyrotropin stimulation of the lutropin/choriogonadotropin receptor: different sites mediate agonist activity and high affinity binding. Thyroid 1994, 4(4):447-457. 10.1089/thy.1994.4.447, 7711510.
36. Levine RJ, Vatten LJ, Horowitz GL, Qian C, Romundstad PR, Yu KF, Hollenberg AN, Hellevik AI, Asvold BO, Karumanchi SA. Pre-eclampsia, soluble fms-like tyrosine kinase 1, and the risk of reduced thyroid function: nested case-control and population based study. BMJ 2009, 339:b4336. 10.1136/bmj.b4336, 2778749, 19920004.
37. Ashoor G, Maiz N, Rotas M, Kametas NA, Nicolaides K. Maternal thyroid function at 11 to 13 weeks of gestation and subsequent development of preeclampsia. Prenat Diagn 2010, 30(11):1032-1038. 10.1002/pd.2566, 20865794.
38. Wilson KL, Casey BM, McIntire DD, Halvorson LM, Cunningham FG. Subclinical thyroid disease and the incidence of hypertension in pregnancy. Obstet Gynecol 2012, 119(2 Pt 1):315-320.
39. Haddow JE, Palomaki GE, Allan WC, et al. Maternal thyroid deficiency during pregnancy and subsequent neuropsychological development of the child. New Eng J Med 1999, 341:549-555. 10.1056/NEJM199908193410801, 10451459.
40. Mincheva-Nilsson L, Baranov V. The role of placental exosomes in reproduction. Am J Reprod Immunol 2010, 63:520-533. 10.1111/j.1600-0897.2010.00822.x, 20331583.
41. Frängsmyr L, Baranov V, Nagaeva O, Stendahl U, Kjellberg L, Mincheva-Nilsson L. Cytoplasmic microvesicular form of Fas ligand in human early placenta: switching the tissue immune privilege hypothesis from cellular to vesicular level. Mol Hum Reprod 2005, 11:35-41.
42. Hedlund M, Stenqvist AC, Nagaeva O, Kjellberg L, Wulf M, Baranov V, Mincheva-Nilsson L. Human placenta expresses and secretes NKG2D ligands via exosomes that down-modulate the cognate receptor expression: evidence for immunosuppressive function. J Immunol 2009, 183:340-351. 10.4049/jimmunol.0803477, 19542445.