Cryptosporidium scrofarum n. sp. (Apicomplexa: Cryptosporidiidae) in domestic pigs (Sus scrofa)

Veterinary Parasitology

Volumn 191, Issue 3-4, 2013, Pages 218-227

  Kváč, Martin ^a,b   Kestřánová, Michaela ^b   Pinková, Martina ^c   Květoňová, Dana ^a   Kalinová, Jana ^a   Wagnerová, Pavla ^a,b   Kotková, Michaela ^a   Vítovec, Jiří ^b   Ditrich, Oleg ^a   McEvoy, John ^d   Stenger, Brianna ^d   Sak, Bohumil ^a  

^a INSTITUTE OF PARASITOLOGY   (Czech Republic)

^b UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

^c INSTITUTE OF ANIMAL PHYSIOLOGY AND GENETICS   (Czech Republic)

^d NORTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

Cryptosporidium pig genotype II; Cryptosporidium scrofarum; Molecular analyses; Morphology; Taxonomy; Transmission studies

Indexed keywords

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM PARVUM; CRYPTOSPORIDIUM SCROFARUM; DISEASE SEVERITY; DNA EXTRACTION; DOMESTIC PIG; FECES ANALYSIS; GENOTYPE; HISTOPATHOLOGY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OOCYST; PHYLOGENY; POLYMERASE CHAIN REACTION;

ANIMALS; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; FECES; GENES, PROTOZOAN; GENOTYPE; GERBILLINAE; GUINEA PIGS; MICE; MICE, INBRED BALB C; MICE, SCID; PHYLOGENY; SPECIES SPECIFICITY; SWINE; SWINE DISEASES;

APICOMPLEXA; APODEMUS FLAVICOLLIS; CAVIA; CAVIA PORCELLUS; CRYPTOSPORIDIIDAE; CRYPTOSPORIDIUM; MASTOMYS COUCHA; MERIONES UNGUICULATUS; MUS; SUIDAE; SUS SCROFA; SUS SCROFA DOMESTICA;

EID: 84870955157     PISSN: 03044017     EISSN: 18732550     Source Type: Journal    
DOI: 10.1016/j.vetpar.2012.09.005     Document Type: Article

References (69)

1. Adesiyun A.A., Kaminjolo J.S., Ngeleka M., Mutani A., Borde G., Harewood W., Harper W. A longitudinal study on enteropathogenic infections of livestock in Trinidad. Rev. Soc. Bras. Med. Trop. 2001, 34:29-35.
2. Arrowood M.J., Sterling C.R. Isolation of Cryptosporidium oocysts and sporozoites using discontinuous sucrose and isopycnic Percoll gradients. J. Parasitol. 1987, 73:314-319.
3. Atwill E.R., Sweitzer R.A., Pereira M.G., Gardner I.A., Van V.D., Boyce W.M. Prevalence of and associated risk factors for shedding Cryptosporidium parvum oocysts and Giardia cysts within feral pig populations in California. Appl. Environ. Microbiol. 1997, 63:3946-3949.
4. Bergeland M.E. Necrotic Enteritis in Nursing Piglets, vol. 20 1977, American Association of Veterinary Laboratory Diagnosticians, pp. 151-158.
5. Bilic H.R., Bilkei G. Balantidium, Cryptosporidium and Giardia species infections in indoor and outdoor pig production units in Croatia. Vet. Rec. 2006, 158:61.
6. Budu-Amoako E., Greenwood S.J., Dixon B.R., Barkema H.W., Hurnik D., Estey C., McClure J.T. Occurrence of Giardia and Cryptosporidium in pigs on Prince Edward Island, Canada. Vet. Parasitol. 2012, 184:18-24.
7. Canestri Trotti G., Pampiglione S., Visconti S. Cryptosporidium sp. and Isospora suis in swine in Italy. Parasitologia 1984, 26:299-304.
8. Chen Z., Mi R., Yu H., Shi Y., Huang Y., Chen Y., Zhou P., Cai Y., Lin J. Prevalence of Cryptosporidium spp. in pigs in Shanghai, China. Vet. Parasitol. 2011, 181:113-119.
9. Ebeid M., Mathis A., Pospischil A., Deplazes P. Infectivity of Cryptosporidium parvum genotype I in conventionally reared piglets and lambs. Parasitol. Res. 2003, 90:232-235.
10. Enemark H.L., Ahrens P., Bille-Hansen V., Heegaard P.M., Vigre H., Thamsborg S.M., Lind P. Cryptosporidium parvum: infectivity and pathogenicity of the 'porcine' genotype. Parasitology 2003, 126:407-416.
11. Epe C., Coati N., Schnieder T. Results of parasitological examinations of faecal samples from horses, ruminants, pigs, dogs, cats, hedgehogs and rabbits between 1998 and 2002. Dtsch. Tierarztl. Wochenschr. 2004, 111:243-247.
12. Farzan A., Parrington L., Coklin T., Cook A., Pintar K., Pollari F., Friendship R., Farber J., Dixon B. Detection and characterization of Giardia duodenalis and Cryptosporidium spp. on swine farms in Ontario, Canada. Foodborne Pathog. Dis. 2011, 8:1207-1213.
13. Fayer R., Santin M. Cryptosporidium xiaoi n. sp. (Apicomplexa: Cryptosporidiidae) in sheep (Ovis aries). Vet. Parasitol. 2009, 164:192-200.
14. Fayer R., Santin M., Trout J.M. Cryptosporidium ryanae n. sp. (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus). Vet. Parasitol. 2008, 156:191-198.
15. Fayer R., Santin M., Xiao L. Cryptosporidium bovis n. sp. (Apicomplexa: Cryptosporidiidae) in cattle (Bos taurus). J. Parasitol. 2005, 91:624-629.
16. Featherstone C.A., Marshall J.A., Giles M., Sayers A.R., Pritchard G.C. Cryptosporidium species infection in pigs in East Anglia. Vet. Rec. 2010, 166:51-52.
17. Fiuza V.R., Gallo S.S., Frazao-Teixeira E., Santin M., Fayer R., Oliveira F.C. Cryptosporidium pig genotype II diagnosed in pigs from the state of Rio De Janeiro, Brazil. J. Parasitol. 2011, 97:146-147.
18. Guselle N.J., Appelbee A.J., Olson M.E. Biology of Cryptosporidium parvum in pigs: from weaning to market. Vet. Parasitol. 2003, 113:7-18.
19. Hamnes I.S., Gjerde B.K., Forberg T., Robertson L.J. Occurrence of Cryptosporidium and Giardia in suckling piglets in Norway. Vet. Parasitol. 2007, 144:222-233.
20. Hsu B.M., Wun H.Y., Hsu C.L. Detection and species identification of Cryptosporidium from Taiwan feeding animals. J. Parasitol. 2008, 94:252-256.
21. Izumiyama S., Furukawa I., Kuroki T., Yamai S., Sugiyama H., Yagita K., Endo T. Prevalence of Cryptosporidium parvum infections in weaned piglets and fattening porkers in Kanagawa Prefecture, Japan. Jpn. J. Infect. Dis. 2001, 54:23-26.
22. Jeníková M., Němejc K., Sak B., Květoňová D., Kváč M. New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II. Vet. Parasitol. 2011, 176:120-125.
23. Jiang J., Alderisio K.A., Xiao L. Distribution of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl. Environ. Microbiol. 2005, 71:4446-4454.
24. Johnson J., Buddle R., Reid S., Armson A., Ryan U.M. Prevalence of Cryptosporidium genotypes in pre and post-weaned pigs in Australia. Exp. Parasitol. 2008, 119:418-421.
25. Kennedy G.A., Kreitner G.L., Strafuss A.C. Cryptosporidiosis in three pigs. J. Am. Vet. Med. Assoc. 1977, 170:348-350.
26. Kilani R.T., Sekla L. Purification of Cryptosporidium oocysts and sporozoites by cesium chloride and Percoll gradients. Am. J. Trop. Med. Hyg. 1987, 36:505-508.
27. Kváč M., Hanzlíková D., Sak B., Květoňová D. Prevalence and age-related infection of Cryptosporidium suis. C. muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic. Vet. Parasitol. 2009, 160:319-322.
28. Kváč M., Kestřánová M., Květoňová D., Kotková M., Ortega Y., McEvoy J., Sak B. Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs. Exp. Parasitol. 2012, 131:107-110.
29. Kváč M., Květoňová D., Sak B., Ditrich O. Cryptosporidium pig genotype II in immunocompetent man. Emerg. Infect. Dis. 2009, 15:982-983.
30. Kváč M., Květoňová D., Salát J., Ditrich O. Viability staining and animal infectivity of Cryptosporidium andersoni oocysts after long-term storage. Parasitol. Res. 2007, 100:213-217.
31. Kváč M., Ondráčková Z., Květoňová D., Sak B., Vítovec J. Infectivity and pathogenicity of Cryptosporidium andersoni to a novel host, southern multimammate mouse (Mastomys coucha). Vet. Parasitol. 2007, 143:229-233.
32. Kváč M., Sak B., Hanzlíková D., Kotilová J., Květoňová D. Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic. Parasitol. Res. 2009, 104:425-428.
33. Kváč M., Vítovec J. Prevalence and pathogenicity of Cryptosporidium andersoni in one herd of beef cattle. J. Vet. Med. B: Infect. Dis. Vet. Public Health 2003, 50:451-457.
34. Langkjær R.B., Vigre H., Enemark H.L., Maddox-Hyttel C. Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark. Parasitology 2007, 134:339-350.
35. Maddox-Hyttel C., Langkjær R.B., Enemark H.L., Vigre H. Cryptosporidium and Giardia in different age groups of Danish cattle and pigs-occurrence and management associated risk factors. Vet. Parasitol. 2006, 141:48-59.
36. McCarthy S., Ng J., Gordon C., Miller R., Wyber A., Ryan U.M. Prevalence of Cryptosporidium and Giardia species in animals in irrigation catchments in the southwest of Australia. Exp. Parasitol. 2008, 118:596-599.
37. Miláček P., Vítovec J. Differential staining of cryptosporidia by aniline-carbol-methyl violet and tartrazine in smears from feces and scrapings of intestinal mucosa. Folia Parasitol. (Praha) 1985, 32:50.
38. Moon H.W., Bemrick W.J. Fecal transmission of calf cryptosporidia between calves and pigs. Vet. Pathol. 1981, 18:248-255.
39. Morgan U.M., Monis P.T., Fayer R., Deplazes P., Thompson R.C. Phylogenetic relationships among isolates of Cryptosporidium: evidence for several new species. J. Parasitol. 1999, 85:1126-1133.
40. Morgan U.M., Monis P.T., Xiao L., Limor J., Sulaiman I., Raidal S., O'Donoghue P., Gasser R., Murray A., Fayer R., Blagburn B.L., Lal A.A., Thompson R.C. Molecular and phylogenetic characterisation of Cryptosporidium from birds. Int. J. Parasitol. 2001, 31:289-296.
41. Němejc K., Sak B., Květoňová D., Hanzal V., Jeníková M., Kváč M. The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic). Vet. Parasitol. 2012, 184:122-125.
42. Ng J., Yang R., McCarthy S., Gordon C., Hijjawi N., Ryan U. Molecular characterization of Cryptosporidium and Giardia in pre-weaned calves in Western Australia and New South Wales. Vet. Parasitol. 2011, 176:145-150.
43. Nunez A., McNeilly F., Perea A., Sanchez-Cordon P.J., Huerta B., Allan G., Carrasco L. Coinfection by Cryptosporidium parvum and porcine circovirus type 2 in weaned pigs. J. Vet. Med. B: Infect. Dis. Vet. Public Health 2003, 50:255-258.
44. Olson M.E., Thorlakson C.L., Deselliers L., Morck D.W., McAllister T.A. Giardia and Cryptosporidium in Canadian farm animals. Vet. Parasitol. 1997, 68:375-381.
45. Quilez J., Sanchez-Acedo C., Clavel A., del Cacho E., Lopez-Bernad F. Comparison of an acid-fast stain and a monoclonal antibody-based immunofluorescence reagent for the detection of Cryptosporidium oocysts in faecal specimens from cattle and pigs. Vet. Parasitol. 1996, 67:75-81.
46. Reid A., Lymbery A., Ng J., Tweedle S., Ryan U. Identification of novel and zoonotic Cryptosporidium species in marine fish. Vet. Parasitol. 2010, 168:190-195.
47. Reinoso R., Becares E. The occurrence of intestinal parasites in swine slurry and their removal in activated sludge plants. Bioresour. Technol. 2008, 99:6661-6665.
48. Rhee J.K., Seu Y.S., Park B.K. Isolation and identification of Cryptosporidium from various animals in Korea. III. Identification of Cryptosporidium baileyi from Korean chicken. Kisaengchunghak Chapchi 1991, 29:315-324.
49. Ryan U.M., Monis P., Enemark H.L., Sulaiman I., Samarasinghe B., Read C., Buddle R., Robertson I., Zhou L., Thompson R.C., Xiao L. Cryptosporidium suis n. sp. (Apicomplexa: Cryptosporidiidae) in pigs (Sus scrofa). J. Parasitol. 2004, 90:769-773.
50. Ryan U.M., Samarasinghe B., Read C., Buddle J.R., Robertson I.D., Thompson R.C. Identification of a novel Cryptosporidium genotype in pigs. Appl. Environ. Microbiol. 2003, 69:3970-3974.
51. Sanford S.E. Enteric cryptosporidial infection in pigs: 184 cases (1981-1985). J. Am. Vet. Med. Assoc. 1987, 190:695-698.
52. Sauch J.F., Flanigan D., Galvin M.L., Berman D., Jakubowski W. Propidium iodide as an indicator of Giardia cyst viability. Appl. Environ. Microbiol. 1991, 57:3243-3247.
53. Suarez-Luengas L., Clavel A., Quilez J., Goni-Cepero M.P., Torres E., Sanchez-Acedo C., del Cacho E. Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain). Vet. Parasitol. 2007, 148:231-235.
54. Tacal J.V., Sobieh M., el-Ahraf A. Cryptosporidium in market pigs in southern California, USA. Vet. Rec. 1987, 120:615-616.
55. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 2011, 28:2731-2739.
56. Tzipori S., Smith M., Makin T., Halpin C. Enterocolitis in piglets caused by Cryptosporidium sp. purified from calf faeces. Vet. Parasitol. 1982, 11:121-126.
57. Upton S.J., Current W.L. The species of Cryptosporidium (Apicomplexa: Cryptosporidiidae) infecting mammals. J. Parasitol. 1985, 71:625-629.
58. Villacorta I., Ares-Mazas E., Lorenzo M.J. Cryptosporidium parvum in cattle, sheep and pigs in Galicia (N.W. Spain). Vet. Parasitol. 1991, 38:249-252.
59. Vítovec J., Hamadejová K., Landová L., Kváč M., Květoňová D., Sak B. Prevalence and pathogenicity of Cryptosporidium suis in pre- and post-weaned pigs. J. Vet. Med. B: Infect. Dis. Vet. Public Health 2006, 53:239-243.
60. Vítovec J., Koudela B. Pathogenesis of intestinal cryptosporidiosis in conventional and gnotobiotic piglets. Vet. Parasitol. 1992, 43:25-36.
61. Wang R., Qiu S., Jian F., Zhang S., Shen Y., Zhang L., Ning C., Cao J., Qi M., Xiao L. Prevalence and molecular identification of Cryptosporidium spp. in pigs in Henan, China. Parasitol. Res. 2010, 107:1489-1494.
62. Wieler L.H., Ilieff A., Herbst W., Bauer C., Vieler E., Bauerfeind R., Failing K., Klos H., Wengert D., Baljer G., Zahner H. Prevalence of enteropathogens in suckling and weaned piglets with diarrhoea in southern Germany. J. Vet. Med. B: Infect. Dis. Vet. Public Health 2001, 48:151-159.
63. Xiao L., Escalante L., Yang C., Sulaiman I., Escalante A.A., Montali R.J., Fayer R., Lal A.A. Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl. Environ. Microbiol. 1999, 65:1578-1583.
64. Xiao L., Fayer R., Ryan U., Upton S.J. Cryptosporidium taxonomy: recent advances and implications for public health. Clin. Microbiol. Rev. 2004, 17:72-97.
65. Xiao L., Herd R.P., Bowman G.L. Prevalence of Cryptosporidium and Giardia infections on two Ohio pig farms with different management systems. Vet. Parasitol. 1994, 52:331-336.
66. Xiao L., Moore J.E., Ukoh U., Gatei W., Lowery C.J., Murphy T.M., Dooley J.S., Millar B.C., Rooney P.J., Rao J.R. Prevalence and identity of Cryptosporidium spp. in pig slurry. Appl. Environ. Microbiol. 2006, 72:4461-4463.
67. Yatswako S., Faleke O.O., Gulumbe M.L., Daneji A.I. Cryptosporidium oocysts and Balantidium coli cysts in pigs reared semi-intensively in Zuru, Nigeria. Pak. J. Biol. Sci. 2007, 10:3435-3439.
68. Yin J., Shen Y., Yuan Z., Lu W., Xu Y., Cao J. Prevalence of the Cryptosporidium pig genotype II in pigs from the Yangtze River Delta, China. PLoS One 2011, 6:e20738.
69. Zintl A., Neville D., Maguire D., Fanning S., Mulcahy G., Smith H.V., De Waal T. Prevalence of Cryptosporidium species in intensively farmed pigs in Ireland. Parasitology 2007, 134:1575-1582.