Hemocompatibility Assessment of 3C-SiC for Cardiovascular Applications

Silicon Carbide Biotechnology

Volumn , Issue , 2012, Pages 153-208

  Schettini, Norelli ^a,b   Jaroszeski, Mark J ^c   West, Leigh ^c   Saddow, Stephen E ^a,d  

^a University of South Florida   (United States)

^b UNIVERSIDAD DEL NORTE   (Colombia)

^c UNIVERSITY OF SOUTH FLORIDA   (United States)

^d UNIVERSITY OF SOUTH FLORIDA   (United States)

Author keywords

3C SiC; Adhesion; Cell proliferation; Circularity; Endothelial cells; Platelet reactivity; Si; Thrombogenicity

Indexed keywords

EID: 84870803481     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-0-12-385906-8.00005-2     Document Type: Chapter

References (136)

1. Williams DF On the mechanisms of biocompatibility. Biomaterials 2008, 29:2941-2953.
2. ISO 10993 Biological evaluation of medical devices-part 1: evaluation and testing.
3. Bayliss SC, Buckberry LD, Harris PJ, Tobin M Nature of the silicon-animal cell interface. J Porous Mater 2000, 7:191-195.
4. ISO 10993 Biological evaluation of medical devices-part 5: test for in vitro cytotoxicity.
5. Michelson AD Platelets 2002, Elsevier Academic Press, San Diego, CA.
6. Surmodics, Inc. Diagnosing hemo(in)compatibility, from clinical and device consideratins to test bench assessment; 2004.
7. Surmodics, Inc. Advancements in blood- and tissue-compatible coatings; 2004.
8. Bolz A, Schaldach M Artificial heart valves: improved blood compatibility by PECVD a-SiC:H coating. Artif Organs 1990, 14(4):260-269.
9. Coletti C, Jaroszeski MJ, Hoff AM, Saddow SE. Culture of mammalian cells on single crystal SiC substrates. Fall Mater Res Soc Meeting Proc. Boston 2006; 950.
10. Pallaoro A, Toccoli T, Coletti C, Coppedè N, Vanzetti L, Canteri R, et al. Study and characterization of different SiC terminations and their interactions with biomolecules In: XVIII Congresso Nazionale sulla Scienza e tecnologia del vuoto, Florence, Italy; 2007.
11. Frewin CL, Jaroszeski M, Weeber E, Muffly KE, Kumar A, Peters M Atomic force microscopy analysis of central nervous system cell morphology on silicon carbide and diamond substrates. J Mol Recognit 2009, 22:380-388.
12. Kalnins U, Erglis A, Dinne I, Kumsars I, Jegere S Clinical outcomes of silicon carbide coated stents in patients with coronary artery disease. Med Sci Monit 2002 February, 8(2):116-120.
13. Reyes M, Waits M, Harvey S, Shishkin Y, Geil B, Wolan JT Growth of 3C-SiC on Si molds for MEMS applications. Mater Sci Forum 2006, 527-529:307-310.
14. Furie B, Furie BC Thrombus formation in vivo. J Clin Invest 2005, 115(12):3355-3362.
15. Lichtman MA, Kipps TJ, Seligsohn U, Kaushansky K, Prchal JT Williams hematology 2006, McGraw-Hill Medical. 7th ed.
16. Frost M, Meyerhoff ME In vivo chemical sensor: tackling biocompatibility. Anal Chem November 2006, 78(21):7370-7377.
17. Goodman SL, Cooper SL, Albrecht RM The effects of adsorbed albumin on platelet spreading. J Biomater Sci Polym 1991, 2:147-159.
18. Moriau M, Lavenne EP, Scheiff JM, Debeys C. The physiological mechanisms of haemostasis. In: Blood platelets. Neuilly-sur-Seine, France; Hologramme ed; 1988.
19. Mao C, Zhao WB, Zhu CH, Zhu AP, Shen J, Li SC In vitro studies of platelet adhesion on UV radiation-treated nylon surface. Carbohydr Polym 2005, 59:19-25.
20. Jin L, Abrahams JP, Skinner R, Petitou M, Pike RN, Carrell RW The anticoagulant activation of antithrombin by heparin. Proc Natl Acad Sci 1997, 94:14683-14688.
21. Okpalugo TIT, Ogwu AA, Maguire PD, McLaughlin JA In vitro blood compatibility of a-C:H:Si and a-C:H thin films. Diamond Relat Mater 2004, 13:1088-1092.
22. Advances in silicon carbide processing and applications 2004, Artech House, Inc., Boston, MA. S.E. Saddow, A. Agarwal (Eds.).
23. Naji A, Harmand M Cytocompatibility of two coating materials, amorphous alumina and silicon carbide, using human differentiated cell cultures. Biomaterials 1991, 12(7):690-694.
24. Yakimova R, Petoral Jr RM, Yazdi GR, Vahlberg C, Spetz AL, Uvdal K, et al. Surface functionalization and biomedical applications based on SiC. J Phys D: Appl Phys 2007 October, 40:6435-6442.
25. Kumari TV, Vasudev U, Kumar A, Menon B Cell surface interactions in the study of biocomaptibility. Trends Biomater Artif Organs 2002, 15(2):37-41.
26. Sagvolden G, Giaever I, Pettersen EO, Feder J Cell adhesion force microscopy. Proc Natl Acad Sci 1999, 96:471-476.
27. Goodman CS Mechanisms and molecules that control growth cone guidance. Annu Rev Neurosci 1996 March, 19:341-377.
28. Williams DF The Williams dictionary of biomaterials 1999, Liverpool University Press.
29. Salvagnini C. Thrombin inhibitors grafting on polyester membranes for the preparation of blood-compatible materials; 2005. Available online at: <> (accessed 13 August 2011). http://hdl.handle.net/2078.1/5328.
30. Hanson SR Blood coagulation and blood-materials interactions. Biomaterials science, an introduction to materials in medicine 2004, 332. Elsevier Academic Press, San Diego, CA. B.D. Ratner, A.S. Hoffman, F.J. Schoen (Eds.).
31. Ratner BD Blood compatibility-a perspective. J Biomater Sci Polym Ed 2000, 11:1107-1119.
32. Schmaier AH Contact activation: a revision. Thromb Haemost 1997, 77:101-107.
33. Hunt J Foreign body response. Encyclopedia of biomaterials and biomedical engineering 2004, Informa Healthcare. L. Bowlin, G. Wnek (Eds.).
34. Szycher M, Sharma CP Blood compatible materials and devices: perspectives towards the 21st century 1990, Lancaster, PA: CRC Press.
35. Ito Y, Liu L-S, Matsuo R, Imanishi Y Synthesis and nonthrombogenicity of polymer membrane with surface-graft polymers carrying thrombin inhibitor. J Biomed Mater Res 1992, 26:1065-1080.
36. Kishida A, Ueno Y, Fukudome N, Yashima E, Maruyama I, Akashi M Immobilization of human thrombomodulin onto poly(ether urethane urea) for developing antithrombogenic blood-contacting materials. Biomaterials 1994, 15:848-852.
37. Garfin DE Gel electrophoresis of proteins. Essential cell biology: cell structure, a practical approach 2003, vol. 1:197-268. Oxford University Press, Oxford.
38. Seyfert UT, Biehl V, Schenk J In vitro hemocomptibility testing of biomaterials according to the ISO 1993-4. Biomol Eng 2002, 19:91-96.
39. ISO 10993 Biological evaluation of medical devices-part 4: selection of tests for interactions with blood, 2002.
40. Buchanan JM, Upman PJ, Wallin RF. MD&DI; 1998. Available online at: <> (accessed 13 August 2011). http://www.devicelink.com/mddi/archive/98/11/009.html.
41. Li X, Bhushan B, Takashima K, Baek C-W, Kim Y-K Micro/nanoscale mechanical and tribological characterization of SiC for orthopedic applications. J Biomed Mater Res B: Appl Biomater 2005, 72(2):353-361.
42. Nurdin N, François P, Mugnier Y, Krumeich J, Moret M, Aronsson B-O Hemocompatibility evaluation of DLC- and SiC-coated surfaces. Eur Cells Mater 2003, 5:17-28.
43. Rosenbloom AJ, Sipe DM, Shishkin Y, Ke Y, Devaty RP, Choyke WJ Nanoporous SiC: a candidate semi-permeable material. Biomed Microdev 2004, 6(4):261-267.
44. Chen JY, Leng YX, Tian XB, Wang LP, Huang N, Chu PK Antithrombogenic investigation of surface energy and optical bandgap and hemocompatibility mechanism of Ti(Ta+5)O2 thin films. Biomaterials 2002, 23:2545-2552.
45. Kotzar G, Freas M, Abel P, Fleischman A, Roy S, Zorman C Evaluation of MEMS materials of construction for implantable medical devices. Biomaterials 2002, 23(13):2737-2750.
46. Weisenberg BA, Mooradian DL Hemocompatibility of materials used in microelectromechanical systems: platelet adhesion and morphology in vitro. J Biomed Mater Res 2002 May, 60(2):283-291.
47. Zhang XG Electrochemistry of silicon and its oxide 2001, Kluwer Academic/Plenum Publishers, New York.
48. Sanderson LAW, Emoto K, Alstine JM, Weimer JJ Characterization of grafted poly(ethylene glycol) on Si wafers using scanning probe microscopy. J Colloid Interface Sci 1998, 207(1).
49. Snellings GMBF, Vansteenkiste SO, Corneillie SI, Davies MC, Schacht EH Protein adhesion at poly(ethylene glycol) modified surfaces. Adv Mater 2000, 12(24):1959-1962.
50. Takami Y, Yamane S, Makinouchi K, Otsuka G, Glueck J, Benkowski R Protein adsorption onto ceramic surfaces. J Biomed Mater Res 1998 April, 40(1).
51. Ali N, Kousar Y, Okpalugo TI, Singh V, Pease M, Ogwu AA Human microvascular endothelial cell seeding on Cr-DLC thin films for heart valve applications. J Mater Eng Perform 2006, 15(2):230-235.
52. Perego G, Preda P, Pasquinelli G, Curti T, Freyrie A, Cenni E Functionalization of poly-(L-lactic-co-epsilon-caprolactone): effects of surface modification on endothelial cell proliferation and hemocompatibility [corrected]. J Biomater Sci Polym Ed 2003, 14(10):1057-1075.
53. Jing FJ, Wang L, Liu YW, Fu RKY, Zhao XB, Shen R Hemocompatibility of lanthanum oxide films fabricated by dual plasma deposition. Thin Solid Films 2006, 515(3):1219-1222.
54. Ruckenstein E, Marmur A, Rakower SR Sedimentation and adhesion of platelets onto a horizontal glass surface. Thromb Haemostas 1976, 36:334-342.
55. Goodman SL, Lelah MD, Lambrecht LK, Cooper SL In vitro v. ex vivo platelet deposition on polymer surfaces. Scan Electron Microsc 1984, I:279-290.
56. Baumgartner HR, Muggli R, Tschopp TB, Turitto VT Platelet adhesion, release and aggregation in flowing blood: effects of surface properties and platelet function. Thromb Haemostas 1976 February, 35(1):124-138.
57. Kamath S, Blann AD, Lip GYH Platelet activation: assessment and quantification. Eur Heart J 2001, 22:1561-1571.
58. Jiang Y, Rongbing B, Ling T, Jian S, Sicong L Blood compatibility of polyurethane surface grafted copolymerization with sulfobetaine monomer. Coll Surf B: Biointerf 2004, 36:27-33.
59. Brass LF, Zhu L, Stalker TJ Minding the gaps to promote thrombus growth and stability. J Clin Invest 2005, 115(12):3385-3392.
60. McFarland JG, Aster RH Evaluation of four methods for platelet compatibility testing. Blood 1987, 69(5):1425-1430.
61. Weber N, Wendel HP, Kohn J Formation of viscoelastic protein layers on polymeric surfaces relevant to platelet adhesion. Biomaterials 2007, 28(35):5246-5258.
62. Wendel HP, Ziemer G Coating-techniques to improve the hemocompatibility of artificial devices used for extracorpreal circulation. Eur J Cardiothorac Surg 1999, 16:342-350.
63. Chappey O, Wautier M-P, Wautier J-L Endothelial cells in culture: a model to study in vitro vascular toxicity. Toxic In Vitro 1995, 9(4):411-419.
64. Bain BJ A beginners guide to blood cells 2004, Blackwell Publishing Ltd, Massachusetts. 2nd ed.
65. Frojmovic MM, Milton JG Human platelet size, shape, and related functions in health and disease. Physiol Rev 1982 January, 62(1):185-261.
66. The journal of biological chemistry; 2003 December. Available online at: <> (accessed 13 August 2011). http://www.jbc.org/content/278/51.cover-expansion.
67. Park K, Mao FW, Park H Morphological characterization of surface induced platelet activation. Biomaterials 1989, 11:24-31.
68. Image J. Available online at: <> (accessed 13 August 2011). http://rsbweb.nih.gov/ij/.
69. Levy-Toledano S, Maclouf J, Bryon P, Savariau EH Human platelet activation in the absence of aggregation: a calcium-dependent phenomenon independent of thromboxane formation. Blood 1982 May, 59(5):1078-1085.
70. Mani H, Luxembourg B, Kläffling C, Erbe M, Lindhoff-Last E Use of native or platelet count adjusted platelet rich plasma for platelet aggregation measurements. J Clin Pathol 2005, 58:747-750.
71. Coletti C, Jaroszeski MJ, Pallaoro A, Hoff AM, Iannotta S, Saddow SE Biocompatibility and wettability of crystalline SiC and Si surfaces. IEEE EMBS Proc 2007, 5849-5852.
72. Hitchcock SJ, Carroll NT, Nicholas MG Some effects of substrate roughness on wettability. J Mater Sci 1981 March, 16(3):714-732.
73. Israelachvili J Intermol ecular and surface forces 1992, Academic Press, London.
74. Freshney RI Culture of animal cells: a manual of basic technique 2005, John Wiley & Sons, Inc. 5th ed.
75. Yao LJ, Mayo KH Interactions of integrin GPIIb/IIIa-derived peptides with fibrinogen investigated by NMR spectroscopy. Biochem J 1996, 315:161-170.
76. Gu Y, Boonstra P, Rijnsburger AA, Haan J, van Oeveren W Cardiopulmonary bypass circuit treated with surface-modifying additives: a clinical evaluation of blood compatibility. Ann Thorac Surg 1998, 65:1342-1347.
77. Gladstone. Integrilin: basic research translated into therapy; 2009 October. Available online at: <> (accessed 13 August 2011). http://www.gladstone.ucsf.edu/gladstone/site/publicaffairs/section.php?i d=1396.
78. Lord MS, Cousins BG, Doherty PJ, Whitelock JM, Simmons A, Williams RL The effect of silica nanoparticulate coatings on serum protein adsorption and cellular response. Biomaterials 2006, 27:4856-4862.
79. KSV. KSV-QCM techinical note. Electronic document; 2004 October.
80. Weber N, Pesnell A, Bolikal D, Zeltinger J, Kohn J Viscoelastic properties of fibrinogen adsorbed to the surface of biomaterials used in blood-contacting medical devices. Langmuir 2007, 23:3298-3304.
81. Q-sense. Q-sense QCM-D technology; 2009. Available online at: <> (accessed 13 August 2011). http://www.q-sense.com/index.asp.
82. Rodahl M, Kasemo BA A simple setup to simultaneously measure the resonant frequency and the absolute dissipation factor of a quartz crystal microbalance. Rev Sci Intrum 1996, 67(9):3238-3241.
83. Marx KA Quartz crystal microbalance: a useful tool for studying thin polymer films and complex biomolecular systems at the solution-surface interface. Biomacromolecules 2003, 4(5):1099-1120.
84. Osterud B Activation pathways of the coagulation system in normal haemostasis. Scan J Haematol 1884, 32:337-345.
85. Tsai WB, Grunkemeier JM, McFarland CD, Horbertt TA Platelet adhesion to polystyrene-based surfaces preadsorbed with plasmas selectively depleted in fibrinogen, fibronectin, or von Willebrand's factor. J Biomater Res 2002, 60(3):348-359.
86. Q-Sense. Hemocompatibility of hydroxyapatite (application note QS405-12-1). Electronic document.
87. Smith JW, Ruggeri ZM, Kunicki TJ, Cheresh DA Interaction of integrins alpha v beta 3 and glycoprotein IIb-IIIa with fibrinogen. Differential peptide recognition accounts for distinct binding sites. J Biol Chem 1990, 265(21):12267-12271.
88. USF FcoE. Center for Drug Discovery and Innovation. Available online at: <> (accessed 13 August 2011). http://www.bitt.usf.edu/.
89. Weber N, Wendel HP, Kohn J Formation of viscoelastic protein layers on polymeric surfaces relevant to platelet adhesion. J Biomed Mater Res Part A 2005, 72A(4):420-427.
90. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P Molecular biology of the cell 2002, Garland Science, New York. 4th ed.
91. McGeachie J. Blue histology-more about endothelial cells; 1998 February. Available online at: <> (accessed 13 August 2011). http://www.lab.anhb.uwa.edu.au/mb140/MoreAbout/Endothel.htm.
92. Richards RG The effect of surface roughness on fibroblast adhesion in vitro. Injury 1996, 27(Suppl. 3):S-C38-S-C48.
93. Mosmann T Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth 1983, 65(1):55-63.
94. Spinner DM. MTT growth assays in ovarian cancer. In: Bartlett, MS, editor. Ovarian cancer: methods and protocols. Humana Press, Inc; p. 175-7.
95. Ahmadian S, Barar J, Saei AA, Fakhree MAA, Omidi Y. Cellular toxicity of nanogenomedicine in MCF-7 cell line: MTT assay. JoVE. 26; 2009. Available online at:<> (accessed 13 August 2011). http://www.jove.com/details.php?id=1191.
96. Coletti C. Silicon carbide biocomaptibility, surface control and electronic cellular interaction for biosensing applications. Ph.D.E.E. dissertation; 2007.
97. Frewin CL, Coletti C, Riedl C, Starke U, Saddow SE A comprehensive study of hydrogen etching on the major SiC polytypes and crystal orientations. Mater Sci Forum 2009, 615-17:589-592.
98. Cascade Biologics. Inc. Human microvascular endothelial cells (adult dermis). Document. Available online at:<> (accessed 13 August 2011). http://www.qingyuanbio.com/UploadFile/C-011-5C_20100120103709609.pdf.
99. Cascade Biologics. Inc. Medium 131, MVGS and AF: instructions for storage and use. Document.
100. Perry CC, Keeling-Tucker T Aspects of the bioinorganic chemistry of silicon in conjunction with the biometals calcium, iron and aluminium. J Inorg Biochem 1998, 69(181).
101. Invitrogen. CellTracker™ probes for long-term tracing of living cells. Document; 2008 February.
102. Barry Arkles. Hydrophobicity, hydrophilicity and silanes. Paint Coatings Ind Mag 2006 October.
103. Mirenghi L, Ramires PA, Pentassuglia RE, Rotolo P, Romito A Growth of human endothelial cells on plasma treated polyethyleneterephthalate surfaces. J Mater Sci: Mater Med 2000, 11:327-331.
104. Kulkarni S, Dopheide SM, Yap CL, Ravanat C, Freund M, Mangin P A revised model of platelet aggregation. J Clin Invest 2000 March, 105(6):783-791.
105. García AJ Intefaces to control cell-biomaterial adhesive interactions. Adv Polym Sci 2006, 203:171-190.
106. Zilla P, Deutsch M, Meinhart J Endothelial cell transplantation. Semin Thromb Hemostais 1999, 12:52-63.
107. Fisher M, Levine PH, Fullerton AL Marker proteins of platelet activation in patients with cerebrovascular disease. Arch Neurol 1982, 39:692-695.
108. Blann AD, Lip GYH Hypothesis: is soluble P-selectin a new marker of platelet activation?. Atherosclerosis 1997, 128:135-138.
109. Chong BH, Murray B, Berndt MC, Dunlop LC, Brighton T, Chesterman CN Plasma P-selectin is increased in thrombotic consumptive platelet disorders. Blood 1994, 83:1535-1541.
110. Corash L Measurement of platelet activation by fluorescence-activated flow cytometry. Blood Cells 1990, 16:97-106.
111. Lazarus AH, Wright JF, Blanchette V, Freedman J Analysis of platelets by flow cytometry. Transfus Sci 1995, 16:353-361.
112. ISO 10993 Biological evaluation of medical devices-part 1: evaluation and testing.
113. ISO 10993 Biological evaluation of medical devices-part 5: test for in vitro cytotoxicity.
114. Surmodics, Inc. Diagnosing hemo(in)compatibility, from clinical and device consideratins to test bench assessment; 2004.
115. Surmodics, Inc. Advancements in blood- and tissue-compatible coatings; 2004.
116. Coletti C, Jaroszeski MJ, Hoff AM, Saddow SE. Culture of mammalian cells on single crystal SiC substrates. Fall Mater Res Soc Meeting Proc. Boston 2006; 950.
117. Pallaoro A, Toccoli T, Coletti C, Coppedè N, Vanzetti L, Canteri R, et al. Study and characterization of different SiC terminations and their interactions with biomolecules In: XVIII Congresso Nazionale sulla Scienza e tecnologia del vuoto, Florence, Italy; 2007.
118. Moriau M, Lavenne EP, Scheiff JM, Debeys C. The physiological mechanisms of haemostasis. In: Blood platelets. Neuilly-sur-Seine, France; Hologramme ed; 1988.
119. Salvagnini C. Thrombin inhibitors grafting on polyester membranes for the preparation of blood-compatible materials; 2005. Available online at: <> (accessed 13 August 2011). http://hdl.handle.net/2078.1/5328.
120. ISO 10993 Biological evaluation of medical devices-part 4: selection of tests for interactions with blood, 2002.
121. Buchanan JM, Upman PJ, Wallin RF. MD&DI; 1998. Available online at: <> (accessed 13 August 2011). http://www.devicelink.com/mddi/archive/98/11/009.html.
122. The journal of biological chemistry; 2003 December. Available online at: <> (accessed 13 August 2011). http://www.jbc.org/content/278/51.cover-expansion.
123. Image J. Available online at: <> (accessed 13 August 2011). http://rsbweb.nih.gov/ij/.
124. Gladstone. Integrilin: basic research translated into therapy; 2009 October. Available online at: <> (accessed 13 August 2011). http://www.gladstone.ucsf.edu/gladstone/site/publicaffairs/section.php?i d=1396.
125. KSV. KSV-QCM techinical note. Electronic document; 2004 October.
126. Q-sense. Q-sense QCM-D technology; 2009. Available online at: <> (accessed 13 August 2011). http://www.q-sense.com/index.asp.
127. Q-Sense. Hemocompatibility of hydroxyapatite (application note QS405-12-1). Electronic document.
128. USF FcoE. Center for Drug Discovery and Innovation. Available online at: <> (accessed 13 August 2011). http://www.bitt.usf.edu/.
129. McGeachie J. Blue histology-more about endothelial cells; 1998 February. Available online at: <> (accessed 13 August 2011). http://www.lab.anhb.uwa.edu.au/mb140/MoreAbout/Endothel.htm.
130. Spinner DM. MTT growth assays in ovarian cancer. In: Bartlett, MS, editor. Ovarian cancer: methods and protocols. Humana Press, Inc; p. 175-7.
131. Ahmadian S, Barar J, Saei AA, Fakhree MAA, Omidi Y. Cellular toxicity of nanogenomedicine in MCF-7 cell line: MTT assay. JoVE. 26; 2009. Available online at:<> (accessed 13 August 2011). http://www.jove.com/details.php?id=1191.
132. Coletti C. Silicon carbide biocomaptibility, surface control and electronic cellular interaction for biosensing applications. Ph.D.E.E. dissertation; 2007.
133. Cascade Biologics. Inc. Human microvascular endothelial cells (adult dermis). Document. Available online at:<> (accessed 13 August 2011). http://www.qingyuanbio.com/UploadFile/C-011-5C_20100120103709609.pdf.
134. Cascade Biologics. Inc. Medium 131, MVGS and AF: instructions for storage and use. Document.
135. Invitrogen. CellTracker™ probes for long-term tracing of living cells. Document; 2008 February.
136. Barry Arkles. Hydrophobicity, hydrophilicity and silanes. Paint Coatings Ind Mag 2006 October.