A modified colorimetric method of gelatinolytic assay using bacterial collagenase type II as a model

Analytical Biochemistry

Volumn 433, Issue 2, 2013, Pages 168-170

  Osathanunkul, Maslin ^a   Buddhachat, Kittisak ^a   Chomdej, Siriwadee ^a  

^a CHIANG MAI UNIVERSITY   (Thailand)

Author keywords

Bacterial collagenase; Colorimetric assay; Coomassie brilliant blue; Gelatinolytic activity

Indexed keywords

DIMETHYL SULFOXIDE;

ABSORBANCES; COLLAGENASE; COLORIMETRIC ASSAYS; COLORIMETRIC METHODS; COOMASSIE BRILLIANT BLUE; INHIBITORY ACTIVITY; LIMIT OF DETECTION; PHARMACEUTICAL AGENTS;

COLORIMETRY;

ASCORBIC ACID; BACTERIAL COLLAGENASE TYPE II; BRILLIANT BLUE; CLOSTRIDIOPEPTIDASE A; DIMETHYL SULFOXIDE; DOXYCYCLINE; GELATIN; SULFOXIDE; UNCLASSIFIED DRUG;

ARTICLE; CLOSTRIDIUM HISTOLYTICUM; COLORIMETRY; CONTROLLED STUDY; DRUG EFFECT; ENZYME ACTIVITY; INCUBATION TIME; LIMIT OF DETECTION; MICROPLATE READER; MICROWAVE RADIATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS);

EID: 84870695160     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.09.036     Document Type: Article

References (14)

1. G. Murphy, J.A. Allan, F. Willenbrock, M.I. Cockett, J.P. O'Connell, and A.J.P. Docherty The role of the c-terminal domain in collagenase and stromelysin specificity J. Biol. Chem. 267 1992 9612 9618
2. P. Reboul, J.P. Pelletier, G. Tardif, J.M. Cloutier, and J.M. Pelletier The new collagenase, collagenase-3, is expressed and synthesized by human chondrocytes but not by synoviocytes J. Clin. Invest. 97 1997 2011 2019
3. H. Yoshioka, I. Oyamada, and G. Usuku An assay of collagenase activity using enzyme linked immunosorbent assay for mammalian collagenase Anal. Biochem. 166 1987 172 177
4. D.M. Bickett, M.D. Green, J. Berman, M. Dezube, A.S. Howe, P.J. Brown, J.T. Roth, and G.M. Mcgeehan A high throughput fluorogenic substrate for interstitial collagenase (MMP-1) and gelatinase (MMP-9) Anal. Biochem. 212 1993 58 64
5. P.A. Snoek-van Beurden, and J.W. Von den Hoff Zymographic techniques for the analysis of matrix metalloproteinases and their inhibitors Biotechniques 38 2005 73 83
6. R. Levenson A collagen film microassay for tissue collagenase Anal. Biochem. 79 1976 579 588
7. A. Nethery, J.G. Lyons, and R.L. O'Grady A spectrophotometric collagenase assay Anal. Biochem. 159 1986 390 395
8. M. Tal, A. Silberstein, and E. Nusser Why does Coomassie brilliant blue R interact differently with different proteins? J. Biol. Chem. 260 1985 9976 9980
9. W.O. Fenn The effects of electrolytes on gelatin and their biological significance J. Biol. Chem. 34 1918 415 428
10. V.J. Nesatyy, A. Daccay, J.F. Kelly, and N.W. Ross Microwave-assisted protein staining: mass spectrometry compatible methods for rapid protein visualisation Rapid Commun. Mass Spectrom. 16 2002 272 280
11. Z. Cheng, J. Moore, and L.L. Yu High-throughput relative DPPH radical scavenging capacity assay J. Agric. Food Chem. 54 2006 7429 7436
12. L.M. Golub, H.M. Lee, M.E. Ryan, W.V. Giannobile, J. Payne, and T. Sorsa Tetracyclines inhibit connective tissue breakdown by multiple non-antimicrobial mechanisms Adv. Dent. Res. 12 1998 12 26
13. R.E.B. Seftor, E.A. Seftor, J.E.D. Larco, D.E. Kleiner, J. Leferson, W.G. Stetler-Stevenson, T.F. McNamara, L.M. Golub, and M.J.C. Hendrix Chemically modified tetracyclines inhibit human melanoma cell invasion and metastasis Clin. Exp. Metastasis 16 1998 217 225
14. J.T. Peterson Matrix metalloproteinase inhibitor development and the remodelling of drug discovery Heart Fail. Rev. 9 2004 63 79